JTB_MOUSE
ID JTB_MOUSE Reviewed; 146 AA.
AC O88824;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Protein JTB;
DE Flags: Precursor;
GN Name=Jtb; Synonyms=Gm622;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10321732; DOI=10.1038/sj.onc.1202510;
RA Hatakeyama S., Osawa M., Omine M., Ishikawa F.;
RT "JTB: a novel membrane protein gene at 1q21 rearranged in a jumping
RT translocation.";
RL Oncogene 18:2085-2090(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, PROTEOLYTIC PROCESSING OF N-TERMINUS,
RP MUTAGENESIS OF 13-ALA-GLY-14, AND INDUCTION.
RX PubMed=17369841; DOI=10.1038/sj.onc.1210423;
RA Kanome T., Itoh N., Ishikawa F., Mori K., Kim-Kaneyama J.R., Nose K.,
RA Shibanuma M.;
RT "Characterization of Jumping translocation breakpoint (JTB) gene product
RT isolated as a TGF-beta1-inducible clone involved in regulation of
RT mitochondrial function, cell growth and cell death.";
RL Oncogene 26:5991-6001(2007).
CC -!- FUNCTION: Required for normal cytokinesis during mitosis. Plays a role
CC in the regulation of cell proliferation. May be a component of the
CC chromosomal passenger complex (CPC), a complex that acts as a key
CC regulator of mitosis. The CPC complex has essential functions at the
CC centromere in ensuring correct chromosome alignment and segregation and
CC is required for chromatin-induced microtubule stabilization and spindle
CC assembly. Increases AURKB activity (By similarity). Inhibits apoptosis
CC induced by TGFB1. Overexpression induces swelling of mitochondria and
CC reduces mitochondrial membrane potential. {ECO:0000250,
CC ECO:0000269|PubMed:17369841}.
CC -!- SUBUNIT: Interacts with AURKA, AURKB, BIRC5 and INCENP. May be a
CC component of the CPC at least composed of BIRC5/survivin,
CC CDCA8/borealin, INCENP and AURKB/Aurora-B (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}. Mitochondrion
CC {ECO:0000269|PubMed:17369841}. Cytoplasm {ECO:0000250}. Cytoplasm,
CC cytoskeleton, microtubule organizing center, centrosome {ECO:0000250}.
CC Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Note=Detected at the
CC centrosome and along spindle fibers during prophase and metaphase.
CC Detected at the midbody during telophase (By similarity).
CC {ECO:0000250}.
CC -!- INDUCTION: Up-regulated by TGFB1 in mammary epithelial cells.
CC {ECO:0000269|PubMed:17369841}.
CC -!- PTM: Undergoes N-terminal proteolytic processing, removing a peptide of
CC about 1 kDa from the N-terminus of the protein.
CC {ECO:0000269|PubMed:17369841}.
CC -!- SIMILARITY: Belongs to the JTB family. {ECO:0000305}.
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DR EMBL; AB016490; BAA33733.1; -; mRNA.
DR EMBL; BC008139; AAH08139.1; -; mRNA.
DR CCDS; CCDS17523.1; -.
DR RefSeq; NP_996807.1; NM_206924.2.
DR AlphaFoldDB; O88824; -.
DR SMR; O88824; -.
DR STRING; 10090.ENSMUSP00000029546; -.
DR iPTMnet; O88824; -.
DR PhosphoSitePlus; O88824; -.
DR PaxDb; O88824; -.
DR PRIDE; O88824; -.
DR ProteomicsDB; 269428; -.
DR Ensembl; ENSMUST00000029546; ENSMUSP00000029546; ENSMUSG00000027937.
DR GeneID; 23922; -.
DR KEGG; mmu:23922; -.
DR UCSC; uc008qbo.1; mouse.
DR CTD; 10899; -.
DR MGI; MGI:1346082; Jtb.
DR VEuPathDB; HostDB:ENSMUSG00000027937; -.
DR eggNOG; KOG4084; Eukaryota.
DR GeneTree; ENSGT00390000016136; -.
DR HOGENOM; CLU_130083_1_0_1; -.
DR InParanoid; O88824; -.
DR OMA; GRRLCWL; -.
DR OrthoDB; 1375062at2759; -.
DR PhylomeDB; O88824; -.
DR TreeFam; TF316934; -.
DR BioGRID-ORCS; 23922; 9 hits in 75 CRISPR screens.
DR ChiTaRS; Jtb; mouse.
DR PRO; PR:O88824; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; O88824; protein.
DR Bgee; ENSMUSG00000027937; Expressed in parotid gland and 255 other tissues.
DR ExpressionAtlas; O88824; baseline and differential.
DR Genevisible; O88824; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0005819; C:spindle; ISO:MGI.
DR GO; GO:0019901; F:protein kinase binding; ISS:UniProtKB.
DR GO; GO:0008637; P:apoptotic mitochondrial changes; IDA:MGI.
DR GO; GO:0000278; P:mitotic cell cycle; ISS:UniProtKB.
DR GO; GO:0000281; P:mitotic cytokinesis; ISS:UniProtKB.
DR GO; GO:0045860; P:positive regulation of protein kinase activity; ISS:UniProtKB.
DR GO; GO:0042127; P:regulation of cell population proliferation; IMP:UniProtKB.
DR InterPro; IPR008657; JTB.
DR PANTHER; PTHR13041; PTHR13041; 1.
DR Pfam; PF05439; JTB; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Membrane;
KW Mitochondrion; Mitosis; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..146
FT /note="Protein JTB"
FT /id="PRO_0000021536"
FT TOPO_DOM 31..105
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..146
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MUTAGEN 13..14
FT /note="AG->DD: Abolishes N-terminal proteolytic
FT processing."
FT /evidence="ECO:0000269|PubMed:17369841"
SQ SEQUENCE 146 AA; 16329 MW; 9E4DAF394B2A2107 CRC64;
MLAGAGRRGL PRAGHLCWLL CAFTLKLCEA EAPVREEKLS VSTSTSPCWL AEEFVVTEEC
TPCSNFQIKT TPECGSTGYV EKITCSSSKR NEFKSCRSAL LEQHLFWKFE GVVVAVALVF
ACLVIVRQRQ LDRKALEKVR KQIESI
