ID   JTX1A_CHIQU             Reviewed;         462 AA.
AC   P58762;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   27-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Toxin CqTX-A {ECO:0000303|PubMed:11866126};
DE            Short=CQT-A;
DE            Short=Toxin A {ECO:0000303|PubMed:11866126};
DE   Flags: Precursor;
OS   Chiropsoides quadrigatus (Box jellyfish) (Chiropsalmus quadrigatus).
OC   Eukaryota; Metazoa; Cnidaria; Cubozoa; Chirodropida; Chiropsalmidae;
OC   Chiropsoides.
OX   NCBI_TaxID=130731;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Nematoblast;
RX   PubMed=11866126; DOI=10.1271/bbb.66.97;
RA   Nagai H., Takuwa-Kuroda K., Nakao M., Oshiro N., Iwanaga S., Nakajima T.;
RT   "A novel protein toxin from the deadly box jellyfish (Sea Wasp, Habu-
RT   kurage) Chiropsalmus quadrigatus.";
RL   Biosci. Biotechnol. Biochem. 66:97-102(2002).
RN   [2]
RP   SUBCELLULAR LOCATION, AND PROBABLE GLYCOSYLATION AT ASN-174.
RX   PubMed=26201970; DOI=10.1159/000434721;
RA   Horiike T., Nagai H., Kitani S.;
RT   "Identification of allergens in the box jellyfish Chironex yamaguchii that
RT   cause sting dermatitis.";
RL   Int. Arch. Allergy Immunol. 167:73-82(2015).
CC   -!- FUNCTION: Critical allergen and main toxic protein of C.quadrigatus
CC       venom (PubMed:26201970). Has potent hemolytic activity
CC       (PubMed:11866126). Is lethal to crayfish (PubMed:11866126). Causes
CC       cutaneous inflammation in humans (PubMed:26201970). May act as a pore-
CC       forming toxin, disrupting normal transmembrane ion concentration
CC       gradients in susceptible cells (By similarity).
CC       {ECO:0000250|UniProtKB:Q9GV72, ECO:0000269|PubMed:11866126,
CC       ECO:0000269|PubMed:26201970}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Heat-stable protein that continues to cause erythematous skin
CC         eruption in the patient and to recognize IgE after heating.
CC         {ECO:0000269|PubMed:26201970};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11866126,
CC       ECO:0000269|PubMed:26201970}. Nematocyst {ECO:0000269|PubMed:11866126,
CC       ECO:0000269|PubMed:26201970}. Target cell membrane {ECO:0000250}.
CC       Note=Forms a membrane channel in the prey. {ECO:0000250}.
CC   -!- PTM: Contains disulfide bonds. {ECO:0000305}.
CC   -!- PTM: N-glycosylated. {ECO:0000269|PubMed:26201970}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE and
CC       IgG(4). {ECO:0000269|PubMed:26201970}.
CC   -!- TOXIC DOSE: LD(50) is 80 ug/kg by intraperitoneal injection into
CC       crayfish. {ECO:0000269|PubMed:11866126}.
CC   -!- SIMILARITY: Belongs to the jellyfish toxin family. Type I subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AB045319; BAB82520.1; -; mRNA.
DR   PIR; JC7805; JC7805.
DR   AlphaFoldDB; P58762; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Allergen; Cytolysis; Disulfide bond; Glycoprotein; Hemolysis;
KW   Ion transport; Membrane; Nematocyst; Secreted; Signal;
KW   Target cell membrane; Target membrane; Toxin; Transmembrane; Transport.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..462
FT                   /note="Toxin CqTX-A"
FT                   /id="PRO_0000035176"
FT   CARBOHYD        174
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498,
FT                   ECO:0000305|PubMed:26201970"
SQ   SEQUENCE   462 AA;  51802 MW;  146172EA25A2458E CRC64;
     MANMLYFSLL ALLFMTGIAS EGTISSGLAS LKAKIDAKRP SGKQLFDKVA NMQKQIEEKF
     SNDDERAKVM GAIGSLSTAV GKFQSGDPAK IASGCLDILV GISSVLKDFA KFSPIFSILS
     MVVGLFSGTK AEESVGSVVK KVVQEQSDQE LQEALYGVKR EYAVSKAFLD GVRNETSDLS
     PTEVSALGAN VPVYQGVRFI AMVVQRIKNR KPRTESEIKR VLSMLELFTD LCSLRDLILL
     DLYQLVATPG HSPNIASGIK EVSNLGREEY KKVFEDLLKT NDKETYLFLS YLYPRERNEQ
     SQKIFKFFDL MKVKYDDRLK QDLTGIQVFS SLHWPNYFLC SSKDYLALIC TKPYGSLRLD
     KLNDGFYSIK TTQSNPKVCH RYGEYILFTH DRNDDLEKFN FVPVKLGERK IYLLSSKASP
     NKFAYVPKTA KGDLFFVDGI PSQLGYGNQG YFTLATDENE QT