ID   JTX21_CARRA             Reviewed;         450 AA.
AC   Q9GV72;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Toxin CrTX-A {ECO:0000303|PubMed:10964707};
DE   AltName: Full=CRT-1;
DE   AltName: Full=CrTX-B {ECO:0000303|PubMed:10964707};
DE   AltName: Full=Toxin 1;
DE   AltName: Full=Toxin-A {ECO:0000303|PubMed:10964707};
DE   AltName: Full=Toxin-B {ECO:0000303|PubMed:10964707};
DE   Flags: Precursor;
OS   Carybdea rastonii (Box jellyfish).
OC   Eukaryota; Metazoa; Cnidaria; Cubozoa; Carybdeida; Carybdeidae; Carybdea.
OX   NCBI_TaxID=78582;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, TOXIC DOSE, FUNCTION,
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Tentacle;
RX   PubMed=10964707; DOI=10.1006/bbrc.2000.3353;
RA   Nagai H., Takuwa K., Nakao M., Ito E., Miyake M., Noda M., Nakajima T.;
RT   "Novel proteinaceous toxins from the box jellyfish (sea wasp) Carybdea
RT   rastoni.";
RL   Biochem. Biophys. Res. Commun. 275:582-588(2000).
CC   -!- FUNCTION: Has potent hemolytic activity (PubMed:10964707). Is lethal to
CC       mice and crayfish (PubMed:10964707). Causes cutaneous inflammation in
CC       humans (PubMed:10964707). May act as a pore-forming toxin, disrupting
CC       normal transmembrane ion concentration gradients in susceptible cells
CC       (Probable). {ECO:0000269|PubMed:10964707, ECO:0000305|PubMed:10964707}.
CC   -!- SUBUNIT: Oligomer. {ECO:0000250|UniProtKB:A7L035}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10964707}.
CC       Nematocyst {ECO:0000269|PubMed:10964707}. Target cell membrane
CC       {ECO:0000250}. Note=Forms a membrane channel in the prey.
CC       {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: It is suggested that CrTX-B is synthesized in the
CC       tentacle, is modified (become CrTX-A) and then migrates to the
CC       nematocyst. {ECO:0000305|PubMed:10964707}.
CC   -!- PTM: Contains disulfide bonds. {ECO:0000305}.
CC   -!- TOXIC DOSE: LD(50) is 20 ug/kg by intravenous injection into mice and 5
CC       ug/kg by intraperitoneal injection into crayfish.
CC       {ECO:0000269|PubMed:10964707}.
CC   -!- MISCELLANEOUS: CrTX-A and CrTX-B are encoded by the same gene. However,
CC       they differ by their molecular masses (43 and 46 kDa, respectively). In
CC       addition, CrTX-A is primarily localized in nematocysts, whereas CrTX-B
CC       is only detected in the tentacle without nematocyst.
CC       {ECO:0000269|PubMed:10964707}.
CC   -!- SIMILARITY: Belongs to the jellyfish toxin family. Type II subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB015878; BAB12728.1; -; mRNA.
DR   PIR; JC7371; JC7371.
DR   AlphaFoldDB; Q9GV72; -.
DR   PRIDE; Q9GV72; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cytolysis; Direct protein sequencing; Disulfide bond; Hemolysis;
KW   Ion transport; Membrane; Nematocyst; Secreted; Signal;
KW   Target cell membrane; Target membrane; Toxin; Transmembrane; Transport.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..450
FT                   /note="Toxin CrTX-A"
FT                   /evidence="ECO:0000305|PubMed:10964707"
FT                   /id="PRO_0000035175"
SQ   SEQUENCE   450 AA;  49392 MW;  CD393CF25BEFD2FD CRC64;
     MILKHLPWLF IVLAITSAKH GKRSDVNSLL TKVETALKEA SGSNEAALEA LEGLKGEIQT
     KPDRVGQATK ILGSVGSALG KLNSGDATKI ISGCLDIVAG IATTFGGPVG MGIGAVASFV
     SSILSLFTGS SAKNSVAAVI DRALSKHRDE AIQRHAAGAK RDFAESSAFI QVMKQQSNLT
     DSDLSIIAAN VPVYKFSNFI GQLESRISQG AATTSLSDAK RAVDFILLYC QLVVMRETLL
     VDLAILYRKG NAEHVASAVE NANRVNKELA ADTLDFLHKL IPEQALIGAV YHPISASETS
     KAILNYTKYF GVPDVPRPIG NRRYKFTNSY WNTYSICSEA YMGNYMFRGC SNVRNPNIRV
     SKMSDGFYTM ENSDRRKLYI TKHDQGWGWG TLDEDPGDQG HMRFIPLRHG KYMVSSKRWP
     NWFMYMESSA SGYIRSWENN PGPQGHWSIT