ID   JTX2A_CARAL             Reviewed;         463 AA.
AC   Q9GNN8; Q54AF4;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Toxin CaTX-A {ECO:0000303|PubMed:10964708};
DE            Short=Toxin-A {ECO:0000303|PubMed:10964708};
DE   AltName: Full=CAT-1 {ECO:0000312|EMBL:BAB12727.1, ECO:0000312|EMBL:BAB87816.1};
DE   AltName: Full=Toxin CaTX-B {ECO:0000303|PubMed:10964708};
DE            Short=Toxin-B {ECO:0000303|PubMed:10964708};
DE   Flags: Precursor;
OS   Carybdea alata (Hawaiian box jellyfish).
OC   Eukaryota; Metazoa; Cnidaria; Cubozoa; Carybdeida; Alatinidae; Alatina.
OX   NCBI_TaxID=1193083;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 158-168 AND 431-448, TOXIC
RP   DOSE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND FUNCTION.
RC   TISSUE=Tentacle;
RX   PubMed=10964708; DOI=10.1006/bbrc.2000.3352;
RA   Nagai H., Takuwa K., Nakao M., Sakamoto B., Crow G.L., Nakajima T.;
RT   "Isolation and characterization of a novel protein toxin from the Hawaiian
RT   box jellyfish (sea wasp) Carybdea alata.";
RL   Biochem. Biophys. Res. Commun. 275:589-594(2000).
CC   -!- FUNCTION: Has potent hemolytic activity (PubMed:10964708). Is lethal to
CC       crayfish (PubMed:10964708). Causes cutaneous inflammation in humans
CC       (Probable). May act as a pore-forming toxin, disrupting normal
CC       transmembrane ion concentration gradients in susceptible cells
CC       (Probable). {ECO:0000269|PubMed:10964708, ECO:0000305|PubMed:10964708}.
CC   -!- SUBUNIT: Oligomer. {ECO:0000250|UniProtKB:A7L035}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10964708}.
CC       Nematocyst {ECO:0000269|PubMed:10964708}. Target cell membrane
CC       {ECO:0000250}. Note=Forms a membrane channel in the prey.
CC       {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: It is suggested that CaTX-B is synthesized in the
CC       tentacle, is modified (become CaTX-A) and then migrates to the
CC       nematocyst. {ECO:0000250|UniProtKB:Q9GV72,
CC       ECO:0000305|PubMed:10964708}.
CC   -!- PTM: Contains disulfide bonds. {ECO:0000305}.
CC   -!- TOXIC DOSE: LD(50) is 5-25 ug/kg by intraperitoneal injection into
CC       crayfish. {ECO:0000269|PubMed:10964708}.
CC   -!- MISCELLANEOUS: CaTX-A and CaTX-B are probably encoded by the same gene
CC       (Probable). They differ by their molecular masses (43 and 45 kDa,
CC       respectively) (PubMed:10964708). In addition, CaTX-A is primarily
CC       localized in nematocysts, whereas CaTX-B is only detected in the
CC       tentacles without nematocyst (PubMed:10964708).
CC       {ECO:0000269|PubMed:10964708, ECO:0000305|PubMed:10964708}.
CC   -!- SIMILARITY: Belongs to the jellyfish toxin family. Type II subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AB036714; BAB12727.1; -; mRNA.
DR   EMBL; AB038156; BAB87816.1; -; mRNA.
DR   PIR; JC7372; JC7372.
DR   AlphaFoldDB; Q9GNN8; -.
DR   SMR; Q9GNN8; -.
DR   TCDB; 1.C.112.1.1; the cubozoan protein toxin (cpt) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   InterPro; IPR036716; Pest_crys_N_sf.
PE   1: Evidence at protein level;
KW   Cytolysis; Direct protein sequencing; Disulfide bond; Hemolysis;
KW   Ion transport; Membrane; Nematocyst; Secreted; Signal;
KW   Target cell membrane; Target membrane; Toxin; Transmembrane; Transport.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..463
FT                   /note="Toxin CaTX-A"
FT                   /evidence="ECO:0000305|PubMed:10964708"
FT                   /id="PRO_0000035174"
SQ   SEQUENCE   463 AA;  51605 MW;  461253DF6CE9D3C0 CRC64;
     MSRGYSLHLV LFLVLSTAFP SQARLSRYRR SAADAVSTDI DGIIGQLNDL GTDTKRLKEA
     LQGVQEAVKK EPATTIAKVS TIVGSVGGSL SKFKSGDPFD VASGCLDIIA SVATTFGGPY
     GIAIGAVASL ISSILSLFSG NSMGSAIKQV IDDAFKKYRD QELEDNVKGA KRTFNAVITF
     VNSVSKTENL TEVHLDSVRD AVRVDAFTNM LGVLESRINR GSVSTDNNEA MRTINFIFLY
     LQLSVMRETL LTQVILLYKR AGGAYDELAL SLSLTSDQNK EATRETVTFL HQMETKYSLC
     GSYYYPIDHS KAAIGILKLT KFFGVPDPAR YTFDGLYYRM QNRAWNRYSI CKESYAGNHM
     FRGCKDSSYH GIRIKKLENG YHTITLRSKA MYVTKHAQGW GWGTADEDPG EQGYFTFIPL
     TNGFYMVSTK KWPDYFVYME SSAHGYIRSW HYNPDPQGQW KIL