ID   JTXU1_CYACP             Reviewed;         243 AA.
AC   P0DUW6;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   29-SEP-2021, sequence version 1.
DT   03-AUG-2022, entry version 4.
DE   RecName: Full=Toxin CcTX-1 {ECO:0000303|PubMed:21333668};
DE   Flags: Fragments;
OS   Cyanea capillata (Lion's mane jellyfish) (Cyanea arctica).
OC   Eukaryota; Metazoa; Cnidaria; Scyphozoa; Semaeostomeae; Cyaneidae; Cyanea.
OX   NCBI_TaxID=27804;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Nematoblast, and Tentacle;
RX   PubMed=21333668; DOI=10.1016/j.toxicon.2011.02.004;
RA   Lassen S., Helmholz H., Ruhnau C., Prange A.;
RT   "A novel proteinaceous cytotoxin from the northern Scyphozoa Cyanea
RT   capillata (L.) with structural homology to cubozoan haemolysins.";
RL   Toxicon 57:721-729(2011).
CC   -!- FUNCTION: Has potent hemolytic activity. Is lethal to crayfish. Causes
CC       cutaneous inflammation in humans. May act as a pore-forming toxin,
CC       disrupting normal transmembrane ion concentration gradients in
CC       susceptible cells. {ECO:0000250|UniProtKB:Q9GNN8}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21333668}.
CC       Nematocyst {ECO:0000269|PubMed:21333668}. Target cell membrane.
CC       Note=Forms a membrane channel in the prey. {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Nematocytes. {ECO:0000305}.
CC   -!- PTM: Contains disulfide bonds. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=31173; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:21333668};
CC   -!- SIMILARITY: Belongs to the jellyfish toxin family. {ECO:0000305}.
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DR   SMR; P0DUW6; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cytolysis; Direct protein sequencing; Disulfide bond; Hemolysis;
KW   Ion transport; Membrane; Nematocyst; Secreted; Target cell membrane;
KW   Target membrane; Toxin; Transmembrane; Transport.
FT   CHAIN           <1..243
FT                   /note="Toxin CcTX-1"
FT                   /evidence="ECO:0000269|PubMed:21333668"
FT                   /id="PRO_0000453744"
FT   REGION          1..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..25
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_CONS        24..25
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        43..44
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        63..64
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        81..82
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        99..100
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        116..117
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        126..127
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        148..149
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        163..164
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        192..193
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        213..214
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_CONS        230..231
FT                   /evidence="ECO:0000305|PubMed:21333668"
FT   NON_TER         1
FT                   /evidence="ECO:0000305|PubMed:21333668"
SQ   SEQUENCE   243 AA;  27654 MW;  9AE352E47B356058 CRC64;
     SSPEKKNDMS KPGRMRFDNK KEPRSSAKNS GNGYGCVDVN AGREPLTGPG KYPSSFLLLL
     EHRKPPDKDR DRWDNVKGCE RKDGKSHWYD SMLKHRSPRE KTLVGSGKDS TNFVYRLLTE
     PLLNERAGCK VVDMWYSLSY WMDVVNKRFG LCHYLGSMMD HGRWNDYSLC KDSYAGKHKP
     AGGPPTPRLK NREECKFYWC WSGSEERAGG MGREYVMHSG GLYGDLTDKR DDPEPNGNLE
     GGV