ID   JURTX_AVIJU             Reviewed;          38 AA.
AC   B3EWQ0;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=U-theraphotoxin-Aju1a;
DE            Short=U-TRTX-Aju1a;
DE   AltName: Full=Juruin {ECO:0000303|PubMed:22973266};
OS   Avicularia juruensis (Yellow-banded pinktoe).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Avicularia.
OX   NCBI_TaxID=548547;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, DISULFIDE BOND, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=22973266; DOI=10.3389/fmicb.2012.00324;
RA   Ayroza G., Ferreira I.L.C., Sayegh R.S.R., Tashima A.K., Silva jr P.I.;
RT   "Juruin: an antifungal juruentoxin peptide from the venom of the amazonian
RT   pink toe spider, Avicularia juruensis, which contains the inhibitory
RT   cystine knot motif.";
RL   Front. Microbiol. 3:324-324(2012).
CC   -!- FUNCTION: Has strong antifungal activity against C.albicans MDM8,
CC       C.krusei IOC 4559 (MIC=2.5-5 uM), C.glabrata IOC 45658 (MIC=2.5-5 uM),
CC       C.albicans IOC 45588 (MIC=2.5-5 uM), C.parapsilosis IOC 456416
CC       (MIC=2.5-5 uM), C.tropicalis IOC 45608 (MIC=2.5-5 uM), C.guilliermondii
CC       IOC 455716 (MIC=2.5-5 uM) and A.niger (MIC=5-10 uM). Lacks antifungal
CC       activity against B.bassiana. Has no antibacterial effect against Gram-
CC       positive bacteria M.luteus, S.epidermidis, S.aureus or against Gram-
CC       negative bacteria E.coli and P.aeruginosa. Has no hemolytic activity
CC       against human erythrocytes. Probable ion channel inhibitor (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22973266}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:D2Y2I3}.
CC   -!- MASS SPECTROMETRY: Mass=4005.83; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:22973266};
CC   -!- SIMILARITY: Belongs to the neurotoxin 12 (Hwtx-2) family. 03 (juruin)
CC       subfamily. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC       URL="https://wangapd3.com/database/query_output.php?ID=02161";
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DR   AlphaFoldDB; B3EWQ0; -.
DR   SMR; B3EWQ0; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR012625; Toxin_20.
DR   Pfam; PF08089; Toxin_20; 1.
DR   PROSITE; PS60022; HWTX_2; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW   Ion channel impairing toxin; Knottin; Secreted; Toxin.
FT   PEPTIDE         1..38
FT                   /note="U-theraphotoxin-Aju1a"
FT                   /evidence="ECO:0000269|PubMed:22973266"
FT                   /id="PRO_0000419529"
FT   DISULFID        3..24
FT                   /evidence="ECO:0000269|PubMed:22973266"
FT   DISULFID        7..30
FT                   /evidence="ECO:0000269|PubMed:22973266"
FT   DISULFID        16..35
FT                   /evidence="ECO:0000269|PubMed:22973266"
SQ   SEQUENCE   38 AA;  4013 MW;  AF08D46104672437 CRC64;
     FTCAISCDIK VNGKPCKGSG EKKCSGGWSC KFNVCVKV