JV_DROME
ID JV_DROME Reviewed; 1912 AA.
AC M9NE38; Q8IQ69;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Protein javelin {ECO:0000312|FlyBase:FBgn0263973};
GN Name=jv {ECO:0000312|FlyBase:FBgn0263973};
GN ORFNames=CG32397 {ECO:0000312|FlyBase:FBgn0263973};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=21930794; DOI=10.1128/mcb.05730-11;
RA Shapira S., Bakhrat A., Bitan A., Abdu U.;
RT "The Drosophila javelin gene encodes a novel actin-associated protein
RT required for actin assembly in the bristle.";
RL Mol. Cell. Biol. 31:4582-4592(2011).
CC -!- FUNCTION: Important for normal assembly of actin bundles during bristle
CC formation. {ECO:0000269|PubMed:21930794}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:21930794}. Note=Colocalizes with actin filaments.
CC {ECO:0000269|PubMed:21930794}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=D {ECO:0000312|FlyBase:FBgn0263973};
CC IsoId=M9NE38-1; Sequence=Displayed;
CC Name=B {ECO:0000312|FlyBase:FBgn0263973}; Synonyms=C
CC {ECO:0000312|FlyBase:FBgn0263973};
CC IsoId=M9NE38-2; Sequence=VSP_058781;
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in abnormal
CC bristle morphology. The bristle ridges are shallower than wild-type and
CC become nonparallel and disorganized near the tip. The tip of the
CC bristle is swollen giving it a javelin-like appearance. In 13% of
CC animals the posterior scutellar bristles are fused.
CC {ECO:0000269|PubMed:21930794}.
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DR EMBL; AE014296; AAN12084.3; -; Genomic_DNA.
DR EMBL; AE014296; AFH04319.1; -; Genomic_DNA.
DR EMBL; AE014296; AFH04320.1; -; Genomic_DNA.
DR RefSeq; NP_001246648.1; NM_001259719.2. [M9NE38-2]
DR RefSeq; NP_001246649.1; NM_001259720.2. [M9NE38-1]
DR RefSeq; NP_729175.3; NM_168171.4. [M9NE38-2]
DR AlphaFoldDB; M9NE38; -.
DR IntAct; M9NE38; 7.
DR PaxDb; M9NE38; -.
DR EnsemblMetazoa; FBtr0307512; FBpp0300167; FBgn0263973. [M9NE38-2]
DR EnsemblMetazoa; FBtr0307513; FBpp0300168; FBgn0263973. [M9NE38-2]
DR EnsemblMetazoa; FBtr0307514; FBpp0300169; FBgn0263973. [M9NE38-1]
DR GeneID; 318008; -.
DR KEGG; dme:Dmel_CG32397; -.
DR UCSC; CG32397-RA; d. melanogaster.
DR CTD; 318008; -.
DR FlyBase; FBgn0263973; jv.
DR VEuPathDB; VectorBase:FBgn0263973; -.
DR eggNOG; ENOG502RZBB; Eukaryota.
DR HOGENOM; CLU_236569_0_0_1; -.
DR OMA; YQSQAMN; -.
DR PhylomeDB; M9NE38; -.
DR SignaLink; M9NE38; -.
DR BioGRID-ORCS; 318008; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 318008; -.
DR PRO; PR:M9NE38; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0263973; Expressed in seminal fluid secreting gland and 13 other tissues.
DR ExpressionAtlas; M9NE38; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0008407; P:chaeta morphogenesis; IMP:FlyBase.
DR GO; GO:0030046; P:parallel actin filament bundle assembly; IMP:FlyBase.
PE 4: Predicted;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Developmental protein;
KW Reference proteome.
FT CHAIN 1..1912
FT /note="Protein javelin"
FT /id="PRO_0000439053"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 89..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 298..377
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 460..515
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 545..586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 764..920
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 965..1010
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1257..1297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1486..1507
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1881..1912
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..32
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 96..130
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 325..374
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 476..515
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 565..585
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 771..868
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 977..1010
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1491..1507
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1889..1912
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 299..318
FT /note="Missing (in isoform B)"
FT /id="VSP_058781"
SQ SEQUENCE 1912 AA; 215031 MW; CFB488E2A108400D CRC64;
MGNGYFRTSQ TSSSSRQREK RGKDSYSYQH NYVDRVRLED TTGGHYHNNQ HQQTTHDPRC
PQLRAAGWRH THKSVSHLDL ATSCHDAAGT GLGRHSQQLH HHHSNQVNQH HHQQQQSHHH
LQHANLHSHH PHAQPHWTQC RVGGAGSGNG QLRNARSLDY TQLEREENAL DIAEFYWRFD
AEAPLDQVDS YAVLPINDNF EPSAATSTAV GSKETTTTVA NKANGSATTN ATTAAANALL
DILGSESCSL RRSRSLAVIR EETFSDLQIG SANSSRRRSQ LIPRARLVNR GFFRESPRSR
HKFQFKQFKK KPPAKLLRLN GKPNQATLVG EQPADQPTAD DTQSQRSNSA TCDSHQQQQQ
QQHQPQQQHQ QQQHRLSGES RDFDVYYDNL KRLDALALNL SEQLHPWHND KSDLESLNSD
YFKNSLHQNH LDQQQPSSLE FEALEQVAAS LLKERPRIYQ SRRQQQKRNN HHSNCLQRHL
DTGGESCPEH SQSSVFPETT TSNSDDQTDS PSLSEQEYDL THIEEIYQQG NNGEESYEII
SLTTTTRTRF ESSEEGEEEQ TGEEVVDSLT TPTEPQTSDS ESTLRQNHNE QLDKLIAYDS
VYLSSEDSSD CTLVGESCES FEQRTFTSCG ESGELETRSL LHISIEDTVY EPQAKQHKKA
TNQEDQPAPL LTTTAKVEAQ IFTQVLKVEH TPKPNILAVV EKRKLKQEEV KHQPPELKRQ
ATDSFVVTAN KSNLAENQYH SLPDVNIGVS LKVCESIDKE LRSSYNQQRQ QQRKEAKKEQ
QVTRAETYDS IRRFGRAHQK ARQREYQERE REREKEASAA LQEKDKERER EKPKINKEFS
AQERQIEVKE TTKSQDIIKE KEQVSRKEEA EDDEEEEPLP PPPPPLTEES QQEDTVADVE
EEDNLSVSIS QPEQPKEAPV IEVANFSKLI ERRAQEIRER QEQIKPSFQI IVTDAQNNII
QKEAIQENKE TSQRIEPKPS PKTNSNSSST CNLVQRPLRR SVSSSSGKPL EHRILSTGAP
IYKARPVRVL PSSSSDSSFS RGKMSRPQIL HVVDGRGGVA GGGAGGTLRR RSSARSIAST
ISSPGGEVAN EYLQKVDAVR CYWNKLAGNE PETLKTEQPA KETQPGIHFQ LGGETTTQGT
DKSAVGNDFC SMMPHPSIEI VELGEGAQKA TIVKAAPERE EDPDEDQDQF DHIRYKVLKS
QQLIRNNFLS TRNKKEAQFD GLIQYLQEYS FQELLSNNNV VIVEPVRTKI ERPLQVGINS
GSTTTPTTVP PPKPPRVVNA SASQPRKTRQ KQTGGAAAKR HFFYQPVRVN RELYEDELPD
PDTVRNVRRF FEQNVLPTPG QGLLVQSQQK FGGSACQLSP KSRRARGYRY LTIDTSYGGA
EEQPKGMEML EEHKAKHWDN ASLSSGISSG VLSSPCGEYH HQESPVMACK DVHVQDVVRR
HNSNAANAKA RAKFASRRTW CMGAGGPGAN ESLYRQIYEN NLGNSEQQEN GEHLEEEDDE
QDDQYEDDVE QDMCENYYVS NDVLAKIREC GSTVTYYGGR VLDKTATSTA APSTKTTQPM
TGSATRSRIR QIEACNVCLP SERCEHRLQT KQAAAKQQQD SYQGIKFKLV KSNSCSSRLE
LAGTGEDEVT ADSEVVRKMV HHFEANQTTA SNDVQVTINS QSQITSTKKE PEEDVPRRQV
TVNNHINVLG DTMPQASQEK AENFNGRPEE NGYGAVETVP TYESQAMNIR LDATENKNIL
QTAAATAAAN KVCRNKNVDL AYTLVKTTTN SPKGKPIEAP RQKIGKQLRE VEENVEKAAV
TKATIKLTKG APSQIIVPVD IHSQASIVAH TAPIPERRLS NASSSNSVVD KTVVRHYVAN
DKSIYERRKY DEIEFEEFEV YDPSKEPPPQ VEEGTDKIPT DAELYDSLDD KM
