ID   JZ12B_CHIGU             Reviewed;          66 AA.
AC   B1P1C6;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=U1-theraphotoxin-Cg1d 2;
DE            Short=U1-TRTX-Cg1d;
DE   AltName: Full=Jingzhaotoxin-12.2;
DE            Short=JZTX-12.2;
DE   AltName: Full=Peptide F4-15.91;
DE   Flags: Precursor;
OS   Chilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys
OS   jingzhao).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Chilobrachys.
OX   NCBI_TaxID=278060;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=18581053; DOI=10.1007/s00018-008-8135-x;
RA   Chen J., Deng M., He Q., Meng E., Jiang L., Liao Z., Rong M., Liang S.;
RT   "Molecular diversity and evolution of cystine knot toxins of the tarantula
RT   Chilobrachys jingzhao.";
RL   Cell. Mol. Life Sci. 65:2431-2444(2008).
RN   [2]
RP   PROTEIN SEQUENCE OF 30-63, IDENTIFICATION BY MASS SPECTROMETRY, AND
RP   AMIDATION AT PRO-63.
RC   TISSUE=Venom;
RX   PubMed=17476710; DOI=10.1002/pmic.200600785;
RA   Liao Z., Cao J., Li S., Yan X., Hu W., He Q., Chen J., Tang J., Xie J.,
RA   Liang S.;
RT   "Proteomic and peptidomic analysis of the venom from Chinese tarantula
RT   Chilobrachys jingzhao.";
RL   Proteomics 7:1892-1907(2007).
CC   -!- FUNCTION: Probable ion channel inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 46 (Jztx-
CC       7/10/12) subfamily. {ECO:0000305}.
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DR   EMBL; EU233857; ABY71676.1; -; mRNA.
DR   AlphaFoldDB; B1P1C6; -.
DR   SMR; B1P1C6; -.
DR   ArachnoServer; AS000804; U1-theraphotoxin-Cg1d.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   InterPro; IPR013140; Huwentoxin_CS1.
DR   Pfam; PF07740; Toxin_12; 1.
DR   PROSITE; PS60021; HWTX_1; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..29
FT                   /evidence="ECO:0000269|PubMed:17476710"
FT                   /id="PRO_0000398417"
FT   PEPTIDE         30..63
FT                   /note="U1-theraphotoxin-Cg1d 2"
FT                   /id="PRO_0000398418"
FT   MOD_RES         63
FT                   /note="Proline amide"
FT                   /evidence="ECO:0000269|PubMed:17476710"
FT   DISULFID        31..46
FT                   /evidence="ECO:0000250"
FT   DISULFID        38..51
FT                   /evidence="ECO:0000250"
FT   DISULFID        45..58
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   66 AA;  7164 MW;  E6554D1CB04C7974 CRC64;
     MKMSALFPIF GLPLLFCNSF AAELKATGRG CGGLMDGCDG KSTFCCSGFN CSPTWKWCVY
     ARPGRR