ID   JZ50B_CHIGU             Reviewed;          74 AA.
AC   B1P1B2;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=U4-theraphotoxin-Cg1a;
DE            Short=U4-TRTX-Cg1a;
DE   AltName: Full=Jingzhaotoxin-50.2;
DE            Short=JZTX-50.2;
DE   AltName: Full=Peptide F7-7.25;
DE   Flags: Precursor;
OS   Chilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys
OS   jingzhao).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Chilobrachys.
OX   NCBI_TaxID=278060;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=18581053; DOI=10.1007/s00018-008-8135-x;
RA   Chen J., Deng M., He Q., Meng E., Jiang L., Liao Z., Rong M., Liang S.;
RT   "Molecular diversity and evolution of cystine knot toxins of the tarantula
RT   Chilobrachys jingzhao.";
RL   Cell. Mol. Life Sci. 65:2431-2444(2008).
RN   [2]
RP   PROTEIN SEQUENCE OF 40-74, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=17476710; DOI=10.1002/pmic.200600785;
RA   Liao Z., Cao J., Li S., Yan X., Hu W., He Q., Chen J., Tang J., Xie J.,
RA   Liang S.;
RT   "Proteomic and peptidomic analysis of the venom from Chinese tarantula
RT   Chilobrachys jingzhao.";
RL   Proteomics 7:1892-1907(2007).
CC   -!- FUNCTION: Probable ion channel inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=3897.0; Method=MALDI; Note=Monoisotopic mass.;
CC       Evidence={ECO:0000269|PubMed:17476710};
CC   -!- SIMILARITY: Belongs to the neurotoxin 36 family. 01 subfamily.
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DR   EMBL; EU233843; ABY71662.1; -; mRNA.
DR   AlphaFoldDB; B1P1B2; -.
DR   SMR; B1P1B2; -.
DR   ArachnoServer; AS000790; U4-theraphotoxin-Cg1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..39
FT                   /evidence="ECO:0000269|PubMed:17476710"
FT                   /id="PRO_0000398512"
FT   PEPTIDE         40..74
FT                   /note="U4-theraphotoxin-Cg1a"
FT                   /id="PRO_0000398513"
FT   DISULFID        42..56
FT                   /evidence="ECO:0000250"
FT   DISULFID        49..61
FT                   /evidence="ECO:0000250"
FT   DISULFID        55..71
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   74 AA;  8294 MW;  B1E4BA86A668FCD6 CRC64;
     MNATIFAFLL LLNLAMHNAT EQSSETDMDD TLLIPEINRG RCIEEGKWCP KKAPCCGRLE
     CKGPSPKQKK CTRP