JZT32_CHIGU
ID JZT32_CHIGU Reviewed; 86 AA.
AC B1P1F5; P0CH44;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 25-MAY-2022, entry version 46.
DE RecName: Full=U18-theraphotoxin-Cg1a;
DE Short=U18-TRTX-Cg1a;
DE AltName: Full=Jingzhaotoxin-32 {ECO:0000303|PubMed:18581053};
DE Short=JZTX-32 {ECO:0000303|PubMed:18581053};
DE AltName: Full=Jingzhaotoxin-9 {ECO:0000305};
DE Short=JzTx-9 {ECO:0000305};
DE AltName: Full=Jingzhaotoxin-IX {ECO:0000303|PubMed:19409400};
DE Short=JzTx-IX {ECO:0000303|PubMed:19409400};
DE AltName: Full=Peptide F6-16.24 {ECO:0000303|PubMed:17476710};
DE Flags: Precursor; Fragment;
OS Chilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys
OS jingzhao).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Mygalomorphae; Theraphosidae; Chilobrachys.
OX NCBI_TaxID=278060;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND FUNCTION.
RC TISSUE=Venom gland;
RX PubMed=18581053; DOI=10.1007/s00018-008-8135-x;
RA Chen J., Deng M., He Q., Meng E., Jiang L., Liao Z., Rong M., Liang S.;
RT "Molecular diversity and evolution of cystine knot toxins of the tarantula
RT Chilobrachys jingzhao.";
RL Cell. Mol. Life Sci. 65:2431-2444(2008).
RN [2]
RP PROTEIN SEQUENCE OF 50-84, AMIDATION AT PHE-84, IDENTIFICATION BY MASS
RP SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=17476710; DOI=10.1002/pmic.200600785;
RA Liao Z., Cao J., Li S., Yan X., Hu W., He Q., Chen J., Tang J., Xie J.,
RA Liang S.;
RT "Proteomic and peptidomic analysis of the venom from Chinese tarantula
RT Chilobrachys jingzhao.";
RL Proteomics 7:1892-1907(2007).
RN [3]
RP PROTEIN SEQUENCE OF 50-84, FUNCTION, MASS SPECTROMETRY, AND AMIDATION AT
RP PHE-84.
RC TISSUE=Venom;
RX PubMed=19409400; DOI=10.1016/j.neuropharm.2009.04.009;
RA Deng M., Kuang F., Sun Z., Tao H., Cai T., Zhong L., Chen Z., Xiao Y.,
RA Liang S.;
RT "Jingzhaotoxin-IX, a novel gating modifier of both sodium and potassium
RT channels from Chinese tarantula Chilobrachys jingzhao.";
RL Neuropharmacology 57:77-87(2009).
CC -!- FUNCTION: Inhibits TTX-sensitive and TTX-insensitive sodium currents
CC (IC(50) is 0.6 uM and 0.95 uM respectively) on rat dorsal root ganglion
CC (DRG) neurons (PubMed:18581053, PubMed:19409400). Inhibits muscular
CC subtypes sodium channels Nav1.4/SCN4A and Nav1.5/SCN5A transiently
CC transfected in to HEK293 cells (IC(50) is 5.42 uM and 0.45 uM
CC respectively). Also blocks Kv2.1/KCNB1 potassium channels expressed in
CC X.laevis oocytes with an IC(50) of 604 nM. Injection of the toxin in
CC mice was immediately followed by general ataxia, lack of response to
CC stimuli and semiparalysis (PubMed:19409400).
CC {ECO:0000269|PubMed:18581053, ECO:0000269|PubMed:19409400}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17476710}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:17476710}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC {ECO:0000250|UniProtKB:P0C247}.
CC -!- MASS SPECTROMETRY: Mass=3951.6; Method=MALDI; Note=Monoisotopic mass.;
CC Evidence={ECO:0000269|PubMed:19409400};
CC -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 47 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABY71705.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; EU233886; ABY71705.1; ALT_INIT; mRNA.
DR AlphaFoldDB; B1P1F5; -.
DR SMR; B1P1F5; -.
DR ArachnoServer; AS000834; U18-theraphotoxin-Cg1a.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR011696; Huwentoxin-1.
DR Pfam; PF07740; Toxin_12; 1.
PE 1: Evidence at protein level;
KW Amidation; Direct protein sequencing; Disulfide bond;
KW Ion channel impairing toxin; Knottin; Potassium channel impairing toxin;
KW Secreted; Signal; Toxin; Voltage-gated potassium channel impairing toxin;
KW Voltage-gated sodium channel impairing toxin.
FT SIGNAL <1..20
FT /evidence="ECO:0000255"
FT PROPEP 21..49
FT /evidence="ECO:0000269|PubMed:17476710,
FT ECO:0000269|PubMed:19409400"
FT /id="PRO_0000398467"
FT PEPTIDE 50..84
FT /note="U18-theraphotoxin-Cg1a"
FT /evidence="ECO:0000269|PubMed:17476710,
FT ECO:0000269|PubMed:19409400"
FT /id="PRO_0000398468"
FT MOD_RES 84
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:17476710,
FT ECO:0000269|PubMed:19409400"
FT DISULFID 51..65
FT /evidence="ECO:0000250|UniProtKB:P0C247"
FT DISULFID 58..70
FT /evidence="ECO:0000250|UniProtKB:P0C247"
FT DISULFID 64..78
FT /evidence="ECO:0000250|UniProtKB:P0C247"
FT NON_TER 1
SQ SEQUENCE 86 AA; 9524 MW; C6304BA2D01D7F93 CRC64;
KASVLITLAV LGVMFVWTSA AELEERGSDQ RDSPALIKSM AKVFQSEERE CTKLLGGCTK
DSECCPHLGC RKKWPYHCGW DGTFGK
