ID   JZTX2_CHIGU             Reviewed;          63 AA.
AC   B1P1B9;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=U7-theraphotoxin-Cg1a;
DE            Short=U7-TRTX-Cg1a;
DE   AltName: Full=Jingzhaotoxin-2;
DE            Short=JZTX-2;
DE   AltName: Full=Peptide F2-32.19;
DE   Flags: Precursor;
OS   Chilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys
OS   jingzhao).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Chilobrachys.
OX   NCBI_TaxID=278060;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 32-63, FUNCTION, MASS
RP   SPECTROMETRY, AND TOXIN TARGET.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=18840410; DOI=10.1016/j.bcp.2008.09.008;
RA   Wang M., Liu Q., Luo H., Li J., Tang J., Xiao Y., Liang S.;
RT   "Jingzhaotoxin-II, a novel tarantula toxin preferentially targets rat
RT   cardiac sodium channel.";
RL   Biochem. Pharmacol. 76:1716-1727(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=18581053; DOI=10.1007/s00018-008-8135-x;
RA   Chen J., Deng M., He Q., Meng E., Jiang L., Liao Z., Rong M., Liang S.;
RT   "Molecular diversity and evolution of cystine knot toxins of the tarantula
RT   Chilobrachys jingzhao.";
RL   Cell. Mol. Life Sci. 65:2431-2444(2008).
RN   [3]
RP   PROTEIN SEQUENCE OF 32-63, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=17476710; DOI=10.1002/pmic.200600785;
RA   Liao Z., Cao J., Li S., Yan X., Hu W., He Q., Chen J., Tang J., Xie J.,
RA   Liang S.;
RT   "Proteomic and peptidomic analysis of the venom from Chinese tarantula
RT   Chilobrachys jingzhao.";
RL   Proteomics 7:1892-1907(2007).
CC   -!- FUNCTION: Inhibits preferentially tetrodotoxin-insensitive sodium
CC       currents (Nav) on rat cardiac myocytes (IC(50) is 0.26 uM) and has
CC       weaker inhibition activity toward tetrodotoxin-sensitive sodium
CC       currents on rat dorsal root ganglion (DRG) sensory neurons (IC(50) is
CC       0.83 uM) and on cockroach dorsal unpaired median (DUM) neurons (IC(50)
CC       is 1.19 uM). Has no significant effect on potassium currents on DRG
CC       neurons. {ECO:0000269|PubMed:18840410}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=3561.13; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:18840410};
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 36 (Jztx-2)
CC       subfamily. {ECO:0000305}.
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DR   EMBL; EU233850; ABY71669.1; -; mRNA.
DR   AlphaFoldDB; B1P1B9; -.
DR   SMR; B1P1B9; -.
DR   ArachnoServer; AS000799; delta-theraphotoxin-Cg3a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   Pfam; PF07740; Toxin_12; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Secreted; Signal; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..31
FT                   /evidence="ECO:0000269|PubMed:17476710,
FT                   ECO:0000269|PubMed:18840410"
FT                   /id="PRO_0000398392"
FT   PEPTIDE         32..63
FT                   /note="U7-theraphotoxin-Cg1a"
FT                   /id="PRO_0000398393"
FT   DISULFID        33..47
FT                   /evidence="ECO:0000250"
FT   DISULFID        40..52
FT                   /evidence="ECO:0000250"
FT   DISULFID        46..59
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   63 AA;  7066 MW;  B50089A75FF15054 CRC64;
     MKTSILFVIF GLALLFALSV AIEMEEEETD RGCGTMWSPC STEKPCCDNF SCQPAIKWCI
     WSP