ID   JZTX3_CHIGU             Reviewed;          63 AA.
AC   P62520; B1P1A1;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Beta/kappa-theraphotoxin-Cg1a;
DE            Short=Beta/kappa-TRTX-Cg1a;
DE   AltName: Full=Jingzhaotoxin-3;
DE   AltName: Full=Jingzhaotoxin-III;
DE            Short=JZTX-III;
DE   AltName: Full=Peptide F5-20.38;
DE   Flags: Precursor;
OS   Chilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys
OS   jingzhao).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Chilobrachys.
OX   NCBI_TaxID=278060;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-63, FUNCTION, DISULFIDE
RP   BONDS, AND MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=15084603; DOI=10.1074/jbc.m401387200;
RA   Xiao Y., Tang J., Yang Y., Wang M., Hu W., Xie J., Zeng X., Liang S.;
RT   "Jingzhaotoxin-III, a novel spider toxin inhibiting activation of voltage-
RT   gated sodium channel in rat cardiac myocytes.";
RL   J. Biol. Chem. 279:26220-26226(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=18581053; DOI=10.1007/s00018-008-8135-x;
RA   Chen J., Deng M., He Q., Meng E., Jiang L., Liao Z., Rong M., Liang S.;
RT   "Molecular diversity and evolution of cystine knot toxins of the tarantula
RT   Chilobrachys jingzhao.";
RL   Cell. Mol. Life Sci. 65:2431-2444(2008).
RN   [3]
RP   PROTEIN SEQUENCE OF 28-63, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=17476710; DOI=10.1002/pmic.200600785;
RA   Liao Z., Cao J., Li S., Yan X., Hu W., He Q., Chen J., Tang J., Xie J.,
RA   Liang S.;
RT   "Proteomic and peptidomic analysis of the venom from Chinese tarantula
RT   Chilobrachys jingzhao.";
RL   Proteomics 7:1892-1907(2007).
RN   [4]
RP   FUNCTION.
RC   TISSUE=Venom;
RX   PubMed=17150181; DOI=10.1016/j.bbrc.2006.11.086;
RA   Yuan C., Yang S., Liao Z., Liang S.;
RT   "Effects and mechanism of Chinese tarantula toxins on the Kv2.1 potassium
RT   channels.";
RL   Biochem. Biophys. Res. Commun. 352:799-804(2007).
RN   [5]
RP   STRUCTURE BY NMR OF 28-63, AND DISULFIDE BONDS.
RC   TISSUE=Venom;
RX   PubMed=17481690; DOI=10.1016/j.toxicon.2007.03.006;
RA   Liao Z., Yuan C., Peng K., Xiao Y., Liang S.;
RT   "Solution structure of Jingzhaotoxin-III, a peptide toxin inhibiting both
RT   Nav1.5 and Kv2.1 channels.";
RL   Toxicon 50:135-143(2007).
CC   -!- FUNCTION: Selectively inhibits activation of voltage-gated sodium
CC       channels (VGSC) (Nav1.5/SCN5A) in rat cardiac myocytes, followed by
CC       shifting activated voltage in a depolarizing direction. The binding
CC       site on VGSC is suggested to be site 4 located at the extracellular S3-
CC       S4 loop of the channel. Also binds to voltage-gated potassium channels
CC       (Kv2.1/KCNB1). {ECO:0000269|PubMed:17150181}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC   -!- MASS SPECTROMETRY: Mass=3919.4; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15084603};
CC   -!- SIMILARITY: Belongs to the neurotoxin 10 (Hwtx-1) family. 44 (Jztx-4)
CC       subfamily. {ECO:0000305}.
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DR   EMBL; EU233832; ABY71651.1; -; mRNA.
DR   PDB; 2I1T; NMR; -; A=28-63.
DR   PDBsum; 2I1T; -.
DR   AlphaFoldDB; P62520; -.
DR   SMR; P62520; -.
DR   ArachnoServer; AS000046; beta/kappa-theraphotoxin-Cg1a.
DR   EvolutionaryTrace; P62520; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011696; Huwentoxin-1.
DR   Pfam; PF07740; Toxin_12; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin;
KW   Potassium channel impairing toxin; Secreted; Signal; Toxin;
KW   Voltage-gated potassium channel impairing toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000269|PubMed:15084603,
FT                   ECO:0000269|PubMed:17476710"
FT   CHAIN           28..63
FT                   /note="Beta/kappa-theraphotoxin-Cg1a"
FT                   /id="PRO_0000035595"
FT   DISULFID        31..46
FT                   /evidence="ECO:0000269|PubMed:17481690"
FT   DISULFID        38..51
FT                   /evidence="ECO:0000269|PubMed:17481690"
FT   DISULFID        45..58
FT                   /evidence="ECO:0000269|PubMed:17481690"
FT   STRAND          34..37
FT                   /evidence="ECO:0007829|PDB:2I1T"
FT   STRAND          39..41
FT                   /evidence="ECO:0007829|PDB:2I1T"
FT   STRAND          49..52
FT                   /evidence="ECO:0007829|PDB:2I1T"
FT   TURN            53..56
FT                   /evidence="ECO:0007829|PDB:2I1T"
FT   STRAND          57..60
FT                   /evidence="ECO:0007829|PDB:2I1T"
SQ   SEQUENCE   63 AA;  6799 MW;  DEAFF6CE516CD18C CRC64;
     MKNTSILFIL GLALLLVLAF EAQVGESDGE CGGFWWKCGR GKPPCCKGYA CSKTWGWCAV
     EAP