ID   J_BPG4                  Reviewed;          25 AA.
AC   P03652;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=DNA-binding protein J;
DE   AltName: Full=J protein;
DE   AltName: Full=Small core protein;
GN   Name=J;
OS   Escherichia phage G4 (Bacteriophage G4).
OC   Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes;
OC   Petitvirales; Microviridae; Bullavirinae; Gequatrovirus.
OX   NCBI_TaxID=10843;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=714153; DOI=10.1038/276236a0;
RA   Godson G.N., Barrell B.G., Staden R., Fiddes J.C.;
RT   "Nucleotide sequence of bacteriophage G4 DNA.";
RL   Nature 276:236-247(1978).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX   PubMed=8642594; DOI=10.1006/jmbi.1996.0121;
RA   McKenna R., Bowman B.R., Iiag L.L., Rossmann M.G., Fane B.A.;
RT   "Atomic structure of the degraded procapsid particle of the bacteriophage
RT   G4: induced structural changes in the presence of calcium ions and
RT   functional implications.";
RL   J. Mol. Biol. 256:736-750(1996).
CC   -!- FUNCTION: Mediates ssDNA packaging into virion, it locates to the
CC       internal surface of the capsid, thereby displacing the internal
CC       scaffolding protein B during virion formation. Protein J binds to and
CC       is packaged with the viral ssDNA. Additionally, protein J plays a role
CC       in viral attachment efficiency to the host cell.
CC       {ECO:0000250|UniProtKB:P69592}.
CC   -!- SUBUNIT: Interacts with F protein. {ECO:0000250|UniProtKB:P69592}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:P69592}. Host
CC       cytoplasm {ECO:0000250|UniProtKB:P69592}. Note=situated at the
CC       interface between the internal surface of the capsid and the nucleic
CC       acid. {ECO:0000250|UniProtKB:P69592}.
CC   -!- SIMILARITY: Belongs to the microviridae J protein family.
CC       {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Virus Particle ExploreR db; Note=Icosahedral capsid
CC       structure;
CC       URL="https://viperdb.scripps.edu/Info_Page.php?VDB=1gff";
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DR   EMBL; V00657; CAA24018.1; -; Genomic_DNA.
DR   PIR; A04259; ZJBPG4.
DR   PDB; 1GFF; X-ray; 3.00 A; 3=1-25.
DR   PDBsum; 1GFF; -.
DR   SMR; P03652; -.
DR   PRIDE; P03652; -.
DR   EvolutionaryTrace; P03652; -.
DR   Proteomes; UP000002140; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR006815; Microvir_J-like.
DR   Pfam; PF04726; Microvir_J; 1.
DR   PIRSF; PIRSF004161; Microvir_J; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Capsid protein; DNA-binding; Host cytoplasm;
KW   Reference proteome; Viral genome packaging; Viral release from host cell;
KW   Virion.
FT   CHAIN           1..25
FT                   /note="DNA-binding protein J"
FT                   /id="PRO_0000164906"
FT   STRAND          20..22
FT                   /evidence="ECO:0007829|PDB:1GFF"
SQ   SEQUENCE   25 AA;  2815 MW;  87B7A8DFFFD6D033 CRC64;
     MKKSIRRSGG KSKGARLWYV GGTQY