K1328_MOUSE
ID K1328_MOUSE Reviewed; 612 AA.
AC Q6NZK5; Q3TJX2; Q571M8;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Protein hinderin;
GN Name=Kiaa1328;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 149-612 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 390-612.
RC TISSUE=Embryonic tail;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20; SER-471 AND SER-558, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Competes with SMC1 for binding to SMC3. May affect the
CC availability of SMC3 to engage in the formation of multimeric protein
CC complexes. {ECO:0000250|UniProtKB:Q86T90}.
CC -!- SUBUNIT: Interacts (via N- and C-terminal domains) with SMC3 (via
CC central hinge region). {ECO:0000250|UniProtKB:Q86T90}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6NZK5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NZK5-2; Sequence=VSP_029816, VSP_029817;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK167257; BAE39373.1; -; mRNA.
DR EMBL; BC066089; AAH66089.1; -; mRNA.
DR EMBL; AK220161; BAD90347.1; -; mRNA.
DR RefSeq; NP_001028704.2; NM_001033532.3. [Q6NZK5-1]
DR AlphaFoldDB; Q6NZK5; -.
DR SMR; Q6NZK5; -.
DR STRING; 10090.ENSMUSP00000095248; -.
DR iPTMnet; Q6NZK5; -.
DR PhosphoSitePlus; Q6NZK5; -.
DR MaxQB; Q6NZK5; -.
DR PaxDb; Q6NZK5; -.
DR PRIDE; Q6NZK5; -.
DR Antibodypedia; 49207; 20 antibodies from 10 providers.
DR Ensembl; ENSMUST00000097643; ENSMUSP00000095248; ENSMUSG00000033632. [Q6NZK5-1]
DR GeneID; 225289; -.
DR KEGG; mmu:225289; -.
DR UCSC; uc008ehg.1; mouse. [Q6NZK5-2]
DR UCSC; uc008ehj.2; mouse. [Q6NZK5-1]
DR MGI; MGI:2147376; AW554918.
DR VEuPathDB; HostDB:ENSMUSG00000033632; -.
DR eggNOG; ENOG502QTY3; Eukaryota.
DR GeneTree; ENSGT00390000011152; -.
DR InParanoid; Q6NZK5; -.
DR OMA; AKPQTYY; -.
DR OrthoDB; 626494at2759; -.
DR PhylomeDB; Q6NZK5; -.
DR BioGRID-ORCS; 225289; 2 hits in 51 CRISPR screens.
DR ChiTaRS; AW554918; mouse.
DR PRO; PR:Q6NZK5; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q6NZK5; protein.
DR Bgee; ENSMUSG00000033632; Expressed in animal zygote and 69 other tissues.
DR ExpressionAtlas; Q6NZK5; baseline and differential.
DR InterPro; IPR032736; Hinderin.
DR PANTHER; PTHR28375; PTHR28375; 1.
DR Pfam; PF15369; KIAA1328; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Phosphoprotein; Reference proteome.
FT CHAIN 1..612
FT /note="Protein hinderin"
FT /id="PRO_0000312299"
FT REGION 462..484
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 509..598
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 90..166
FT /evidence="ECO:0000255"
FT COILED 362..406
FT /evidence="ECO:0000255"
FT COMPBIAS 464..481
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 509..584
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 20
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 178
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86T90"
FT MOD_RES 471
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 527
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86T90"
FT MOD_RES 558
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 192..196
FT /note="ITKKK -> VSGLL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029816"
FT VAR_SEQ 197..612
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029817"
SQ SEQUENCE 612 AA; 69793 MW; F55F6A51776CAB25 CRC64;
MAGAAGPFLP GSAFWSRDFS DEDQSVAYVP GISTEGNTRS RVKLINPKVD VKVKASRVTD
ASVSMESLKG AGDSVAEQNF CKRGMKSASL KDLCLEDKRR IANLIKELAR VSEEKEVTEE
RLKTEQESFE KKIRQLEEQN ELIIKEREAL QLQYRECQEL LSLYQKYLSE QQEKLTLSLS
ELGAARAQEQ QITKKKNTPQ CSLMDLDGSF LSVARPQNYG QTKARPKSAN QVSESFTELR
NNSLRPITLH HPKEDLERMS TKTRTCTYES LGRRLINAAP IEKSLPVELK IKEYPNLPPT
PSSQYCGHKC SESGAYVHEN YHPTNMAPQC CKTHPESCSH CRIPWASQMH DRVILQPRET
DIEKQLSEDR RQQLMLQKME LEIEKERLQH LLAQQETKLL LKQQQLHQSR LDYNWLRTQA
MFKSRELVAD KEFPKPDLDM NGSVSGPSLW KPKCDGWMRG TSTSFKKCPD SPNSGQNQRE
KKTVEFQSRV ENGIQWTCQQ NDICRPQRET VTGVRKDAST SPMSTRSPKE PLTPASLSSQ
HKTSRYETSL LDLVQSLSPN SAPKSQPHPS RAAGTWSTLR PTPQKSIWKK VGTRRSPEDL
EENQILEDIF FI
