K1C28_HUMAN
ID K1C28_HUMAN Reviewed; 464 AA.
AC Q7Z3Y7; Q6ZP84;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Keratin, type I cytoskeletal 28;
DE AltName: Full=Cytokeratin-28;
DE Short=CK-28;
DE AltName: Full=Keratin-25D;
DE Short=K25D;
DE AltName: Full=Keratin-28;
DE Short=K28;
DE AltName: Full=Type I inner root sheath-specific keratin-K25irs4;
GN Name=KRT28 {ECO:0000312|HGNC:HGNC:30842};
GN Synonyms=KRT25D {ECO:0000312|EMBL:CAD91907.1, ECO:0000312|HGNC:HGNC:30842};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000269|PubMed:15617563, ECO:0000312|EMBL:CAD91907.1}
RP NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-62, AND TISSUE SPECIFICITY.
RC TISSUE=Scalp {ECO:0000312|EMBL:CAD91907.1};
RX PubMed=15617563; DOI=10.1111/j.1432-0436.2004.07209006.x;
RA Rogers M.A., Winter H., Langbein L., Bleiler R., Schweizer J.;
RT "The human type I keratin gene family: characterization of new hair
RT follicle specific members and evaluation of the chromosome 17q21.2 gene
RT domain.";
RL Differentiation 72:527-540(2004).
RN [2] {ECO:0000312|EMBL:BAC85240.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Dermoid cancer {ECO:0000312|EMBL:BAC85240.1};
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT human lineage.";
RL Nature 440:1045-1049(2006).
RN [4] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=16874310; DOI=10.1038/sj.jid.5700494;
RA Langbein L., Rogers M.A., Praetzel-Wunder S., Helmke B., Schirmacher P.,
RA Schweizer J.;
RT "K25 (K25irs1), K26 (K25irs2), K27 (K25irs3), and K28 (K25irs4) represent
RT the type I inner root sheath keratins of the human hair follicle.";
RL J. Invest. Dermatol. 126:2377-2386(2006).
CC -!- FUNCTION: Essential for the proper assembly of types I and II keratin
CC protein complexes and the formation of keratin intermediate filaments
CC in the inner root sheath (irs). {ECO:0000250|UniProtKB:A6BLY7}.
CC -!- SUBUNIT: Heterotetramer of two type I and two type II keratins.
CC {ECO:0000305}.
CC -!- INTERACTION:
CC Q7Z3Y7; Q9P2A4: ABI3; NbExp=3; IntAct=EBI-11980489, EBI-742038;
CC Q7Z3Y7; A0A1B0GWI1: CCDC196; NbExp=3; IntAct=EBI-11980489, EBI-10181422;
CC Q7Z3Y7; Q05D60: DEUP1; NbExp=3; IntAct=EBI-11980489, EBI-748597;
CC Q7Z3Y7; P04264: KRT1; NbExp=5; IntAct=EBI-11980489, EBI-298429;
CC Q7Z3Y7; P12035: KRT3; NbExp=5; IntAct=EBI-11980489, EBI-2430095;
CC Q7Z3Y7; P19013: KRT4; NbExp=3; IntAct=EBI-11980489, EBI-2371606;
CC Q7Z3Y7; P13647: KRT5; NbExp=3; IntAct=EBI-11980489, EBI-702187;
CC Q7Z3Y7; P02538: KRT6A; NbExp=5; IntAct=EBI-11980489, EBI-702198;
CC Q7Z3Y7; P04259: KRT6B; NbExp=5; IntAct=EBI-11980489, EBI-740907;
CC Q7Z3Y7; Q3SY84: KRT71; NbExp=5; IntAct=EBI-11980489, EBI-2952676;
CC Q7Z3Y7; Q5XKE5: KRT79; NbExp=3; IntAct=EBI-11980489, EBI-2514135;
CC Q7Z3Y7; P05787: KRT8; NbExp=5; IntAct=EBI-11980489, EBI-297852;
CC Q7Z3Y7; Q6KB66-2: KRT80; NbExp=3; IntAct=EBI-11980489, EBI-11999246;
CC Q7Z3Y7; Q14533: KRT81; NbExp=3; IntAct=EBI-11980489, EBI-739648;
CC Q7Z3Y7; O43790: KRT86; NbExp=3; IntAct=EBI-11980489, EBI-9996498;
CC Q7Z3Y7; Q9BVL2: NUP58; NbExp=3; IntAct=EBI-11980489, EBI-2811583;
CC Q7Z3Y7; Q8N6Y0: USHBP1; NbExp=3; IntAct=EBI-11980489, EBI-739895;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:A6BLY7}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in skin and scalp, and weak
CC expression observed in thymus. In the hair follicle, expressed in Henle
CC layer, Huxley layer and in the irs cuticle. Expression extends from the
CC bulb region up to the point of differentiation into the three layers.
CC Also present in the medulla of beard hair (at protein level).
CC {ECO:0000269|PubMed:15617563, ECO:0000269|PubMed:16874310}.
CC -!- MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar
CC keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the intermediate filament family.
CC {ECO:0000255|PROSITE-ProRule:PRU01188}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC85240.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AJ564207; CAD91907.1; -; mRNA.
DR EMBL; AK129827; BAC85240.1; ALT_INIT; mRNA.
DR EMBL; AC090283; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS11376.1; -.
DR RefSeq; NP_853513.2; NM_181535.3.
DR AlphaFoldDB; Q7Z3Y7; -.
DR SMR; Q7Z3Y7; -.
DR BioGRID; 127825; 24.
DR IntAct; Q7Z3Y7; 20.
DR STRING; 9606.ENSP00000305263; -.
DR GlyGen; Q7Z3Y7; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q7Z3Y7; -.
DR PhosphoSitePlus; Q7Z3Y7; -.
DR SwissPalm; Q7Z3Y7; -.
DR BioMuta; KRT28; -.
DR DMDM; 294862446; -.
DR jPOST; Q7Z3Y7; -.
DR MassIVE; Q7Z3Y7; -.
DR PaxDb; Q7Z3Y7; -.
DR PeptideAtlas; Q7Z3Y7; -.
DR PRIDE; Q7Z3Y7; -.
DR ProteomicsDB; 69095; -.
DR Antibodypedia; 28714; 88 antibodies from 19 providers.
DR DNASU; 162605; -.
DR Ensembl; ENST00000306658.8; ENSP00000305263.7; ENSG00000173908.9.
DR GeneID; 162605; -.
DR KEGG; hsa:162605; -.
DR MANE-Select; ENST00000306658.8; ENSP00000305263.7; NM_181535.3; NP_853513.2.
DR UCSC; uc002hvh.2; human.
DR CTD; 162605; -.
DR GeneCards; KRT28; -.
DR HGNC; HGNC:30842; KRT28.
DR HPA; ENSG00000173908; Tissue enriched (skin).
DR MIM; 616677; gene.
DR neXtProt; NX_Q7Z3Y7; -.
DR OpenTargets; ENSG00000173908; -.
DR PharmGKB; PA134871316; -.
DR VEuPathDB; HostDB:ENSG00000173908; -.
DR eggNOG; ENOG502SHRG; Eukaryota.
DR GeneTree; ENSGT00940000162192; -.
DR HOGENOM; CLU_012560_8_3_1; -.
DR InParanoid; Q7Z3Y7; -.
DR OMA; KGWYDKY; -.
DR OrthoDB; 805081at2759; -.
DR PhylomeDB; Q7Z3Y7; -.
DR TreeFam; TF332742; -.
DR PathwayCommons; Q7Z3Y7; -.
DR Reactome; R-HSA-6805567; Keratinization.
DR Reactome; R-HSA-6809371; Formation of the cornified envelope.
DR SignaLink; Q7Z3Y7; -.
DR BioGRID-ORCS; 162605; 13 hits in 1059 CRISPR screens.
DR ChiTaRS; KRT28; human.
DR GenomeRNAi; 162605; -.
DR Pharos; Q7Z3Y7; Tdark.
DR PRO; PR:Q7Z3Y7; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q7Z3Y7; protein.
DR Bgee; ENSG00000173908; Expressed in skin of abdomen and 11 other tissues.
DR GO; GO:0005856; C:cytoskeleton; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005882; C:intermediate filament; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR InterPro; IPR039008; IF_rod_dom.
DR InterPro; IPR002957; Keratin_I.
DR PANTHER; PTHR23239; PTHR23239; 1.
DR Pfam; PF00038; Filament; 1.
DR PRINTS; PR01248; TYPE1KERATIN.
DR SMART; SM01391; Filament; 1.
DR PROSITE; PS51842; IF_ROD_2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Intermediate filament; Keratin; Reference proteome.
FT CHAIN 1..464
FT /note="Keratin, type I cytoskeletal 28"
FT /id="PRO_0000312705"
FT DOMAIN 86..401
FT /note="IF rod"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01188"
FT REGION 1..85
FT /note="Head"
FT /evidence="ECO:0000255"
FT REGION 86..121
FT /note="Coil 1A"
FT /evidence="ECO:0000255"
FT REGION 122..143
FT /note="Linker 1"
FT /evidence="ECO:0000255"
FT REGION 144..235
FT /note="Coil 1B"
FT /evidence="ECO:0000255"
FT REGION 236..258
FT /note="Linker 12"
FT /evidence="ECO:0000255"
FT REGION 259..397
FT /note="Coil 2"
FT /evidence="ECO:0000255"
FT REGION 398..464
FT /note="Tail"
FT /evidence="ECO:0000255"
FT REGION 443..464
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 52
FT /note="G -> D (in dbSNP:rs7209228)"
FT /id="VAR_056009"
FT VARIANT 62
FT /note="A -> V (in dbSNP:rs4624233)"
FT /evidence="ECO:0000269|PubMed:15617563"
FT /id="VAR_060236"
FT VARIANT 286
FT /note="A -> T (in dbSNP:rs17558995)"
FT /id="VAR_056010"
SQ SEQUENCE 464 AA; 50567 MW; 34BA7243B68EF42C CRC64;
MSLQFSNGSR HVCLRSGAGS VRPLNGGAGF AGSSACGGSV AGSEFSCALG GGLGSVPGGS
HAGGALGNAA CIGFAGSEGG LLSGNEKVTM QNLNDRLASY LDNVRALEEA NAELERKIKG
WYEKYGPGSC RGLDHDYSRY HLTIEDLKNK IISSTTTNAN VILQIDNARL AADDFRLKYE
NELTLHQNVE ADINGLRRVL DELTLCRTDQ ELQYESLSEE MTYLKKNHEE EMKALQCAAG
GNVNVEMNAA PGVDLAVLLN NMRAEYEALA EQNRKDAEAW FNEKSASLQQ QISHDSGAAT
FARSQLTEMR RTLQTLEIQL QSLMATKHSL ECSLTETESN YCTQLAQIQA QIGALEEQLH
QVRTETEGQK LEYEHLLDVK VHLEKEIETY CRLIDGDGNS CSKSKGFGSG SPGNSSKDLS
KTTLVKTVVE ELDQRGKVLS SRIHSIEEKT SKMTNGKTEQ RVPF
