K2C75_MOUSE
ID K2C75_MOUSE Reviewed; 551 AA.
AC Q8BGZ7; B2RQH6;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Keratin, type II cytoskeletal 75;
DE AltName: Full=Cytokeratin-75;
DE Short=CK-75;
DE AltName: Full=Keratin-6 hair follicle;
DE Short=mK6hf;
DE AltName: Full=Keratin-75;
DE Short=K75;
DE AltName: Full=Type II keratin-K6hf;
DE AltName: Full=Type-II keratin Kb18;
GN Name=Krt75; Synonyms=Kb18;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=11489919; DOI=10.1083/jcb.200102079;
RA Wojcik S.M., Longley M.A., Roop D.R.;
RT "Discovery of a novel murine keratin 6 (K6) isoform explains the absence of
RT hair and nail defects in mice deficient for K6a and K6b.";
RL J. Cell Biol. 154:619-630(2001).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=14675170; DOI=10.1111/j.1523-1747.2003.12644.x;
RA Wang Z., Wong P., Langbein L., Schweizer J., Coulombe P.A.;
RT "Type II epithelial keratin 6hf (K6hf) is expressed in the companion layer,
RT matrix, and medulla in anagen-stage hair follicles.";
RL J. Invest. Dermatol. 121:1276-1282(2003).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS OF ASN-159.
RX PubMed=17851587; DOI=10.1038/sj.jid.5701038;
RA Chen J., Jaeger K., Den Z., Koch P.J., Sundberg J.P., Roop D.R.;
RT "Mice expressing a mutant Krt75 (K6hf) allele develop hair and nail defects
RT resembling pachyonychia congenita.";
RL J. Invest. Dermatol. 128:270-279(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a central role in hair and nail formation. Essential
CC component of keratin intermediate filaments in the companion layer of
CC the hair follicle (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:17851587}.
CC -!- SUBUNIT: Heterodimer of a type I and a type II keratin (By similarity).
CC May associate with KRT17. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the companion layer and upper
CC germinative matrix region of the hair follicle and the medulla of the
CC hair shaft. Also expressed in epithelia of the nail bed and fungiform
CC papillae of dorsal tongue epithelium (at protein level).
CC {ECO:0000269|PubMed:11489919, ECO:0000269|PubMed:14675170,
CC ECO:0000269|PubMed:17851587}.
CC -!- MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar
CC keratin, I (acidic) and II (neutral to basic) (40-55 and 56-70 kDa,
CC respectively).
CC -!- MISCELLANEOUS: Its presence in surviving mice lacking Krt6a and Krt6b,
CC may explain the absence of hair and nail defects in such mice.
CC -!- SIMILARITY: Belongs to the intermediate filament family.
CC {ECO:0000255|PROSITE-ProRule:PRU01188}.
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DR EMBL; AK028972; BAC26221.1; -; mRNA.
DR EMBL; AK037233; BAC29769.1; -; mRNA.
DR EMBL; AK076367; BAC36315.1; -; mRNA.
DR EMBL; BC137935; AAI37936.1; -; mRNA.
DR EMBL; BC137936; AAI37937.1; -; mRNA.
DR CCDS; CCDS27856.1; -.
DR RefSeq; NP_579935.1; NM_133357.3.
DR AlphaFoldDB; Q8BGZ7; -.
DR SMR; Q8BGZ7; -.
DR BioGRID; 224537; 5.
DR IntAct; Q8BGZ7; 1.
DR STRING; 10090.ENSMUSP00000036246; -.
DR iPTMnet; Q8BGZ7; -.
DR PhosphoSitePlus; Q8BGZ7; -.
DR jPOST; Q8BGZ7; -.
DR PaxDb; Q8BGZ7; -.
DR PeptideAtlas; Q8BGZ7; -.
DR PRIDE; Q8BGZ7; -.
DR ProteomicsDB; 268945; -.
DR Antibodypedia; 14610; 158 antibodies from 19 providers.
DR DNASU; 109052; -.
DR Ensembl; ENSMUST00000042957; ENSMUSP00000036246; ENSMUSG00000022986.
DR GeneID; 109052; -.
DR KEGG; mmu:109052; -.
DR UCSC; uc007xtp.1; mouse.
DR CTD; 9119; -.
DR MGI; MGI:1923500; Krt75.
DR VEuPathDB; HostDB:ENSMUSG00000022986; -.
DR eggNOG; ENOG502RTYA; Eukaryota.
DR GeneTree; ENSGT00940000155862; -.
DR HOGENOM; CLU_012560_6_1_1; -.
DR InParanoid; Q8BGZ7; -.
DR OMA; RMFFEAE; -.
DR OrthoDB; 824246at2759; -.
DR PhylomeDB; Q8BGZ7; -.
DR TreeFam; TF317854; -.
DR Reactome; R-MMU-6805567; Keratinization.
DR Reactome; R-MMU-6809371; Formation of the cornified envelope.
DR BioGRID-ORCS; 109052; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Krt75; mouse.
DR PRO; PR:Q8BGZ7; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8BGZ7; protein.
DR Bgee; ENSMUSG00000022986; Expressed in hair follicle and 25 other tissues.
DR Genevisible; Q8BGZ7; MM.
DR GO; GO:0001533; C:cornified envelope; IDA:MGI.
DR GO; GO:0005882; C:intermediate filament; ISO:MGI.
DR GO; GO:0045095; C:keratin filament; IBA:GO_Central.
DR GO; GO:0030280; F:structural constituent of skin epidermis; IBA:GO_Central.
DR GO; GO:0002244; P:hematopoietic progenitor cell differentiation; IMP:MGI.
DR GO; GO:0045109; P:intermediate filament organization; IBA:GO_Central.
DR GO; GO:0031424; P:keratinization; IBA:GO_Central.
DR InterPro; IPR018039; IF_conserved.
DR InterPro; IPR039008; IF_rod_dom.
DR InterPro; IPR032444; Keratin_2_head.
DR InterPro; IPR003054; Keratin_II.
DR Pfam; PF00038; Filament; 1.
DR Pfam; PF16208; Keratin_2_head; 1.
DR PRINTS; PR01276; TYPE2KERATIN.
DR SMART; SM01391; Filament; 1.
DR PROSITE; PS00226; IF_ROD_1; 1.
DR PROSITE; PS51842; IF_ROD_2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Intermediate filament; Keratin; Reference proteome.
FT CHAIN 1..551
FT /note="Keratin, type II cytoskeletal 75"
FT /id="PRO_0000314888"
FT DOMAIN 150..463
FT /note="IF rod"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01188"
FT REGION 1..149
FT /note="Head"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 150..185
FT /note="Coil 1A"
FT REGION 186..204
FT /note="Linker 1"
FT REGION 205..297
FT /note="Coil 1B"
FT REGION 298..320
FT /note="Linker 12"
FT REGION 321..459
FT /note="Coil 2"
FT REGION 460..551
FT /note="Tail"
FT REGION 524..551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 401
FT /note="Stutter"
FT MUTAGEN 159
FT /note="Missing: Mice develop hair and nail defects
FT resembling pachyonychia congenita."
FT /evidence="ECO:0000269|PubMed:17851587"
SQ SEQUENCE 551 AA; 59741 MW; 5EFF6DE17985D4AC CRC64;
MSRQSTITFH SGSRRGFSTA SATTPTAGRS RFSSVSVARS SGNSGGLGRI SGIGSGFGSR
SLYNLGGTRR VSIGGCAGSG FRGGFGGRTS SGFGGSSGFA YGGGIGGGFG GPGFSVCPSG
GIQEVTVNQS LLTPLNLQID PTIQRVRKEE REQIKTLNNK FASFIDKVRF LEQQNKVLET
KWNLLQEQGS RTVRQNLEPF FDTYVNDLRR QLDGITAERG RLDAELRNMQ EVVEDFKVRY
EDEINKRAAA ENEFVGLKKD VDSAYMNKVE LEAKVDSLTD QINFYRMIYE AELSQMQNQV
SDTSVVLSMD NNRSLDLDSI IAEVKAQYED IANRSRAEAE SWYQTKYEEL QVTAGRHGDD
LRNTKQEISE MNRMIQRLRS EIDAVKKQCS SLQTAISDAE QRGELALKDA RAKLMELEDA
LQKAKQDMAR LLREYQELMN VKLALDVEIA TYRKLLEGEE CRLSGEGVSP VNISVVTSTV
SSGYGGGANI GGGSLGLGGN SGYSFTTSGG HSLGTGLGGS GFTTTSSRGP VGSGSSIKFV
SSTSSRKSYK H
