K2C8_DANRE
ID K2C8_DANRE Reviewed; 520 AA.
AC Q6NWF6; Q7ZT78;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Keratin, type II cytoskeletal 8;
DE AltName: Full=Cytokeratin-8;
DE Short=CK-8;
DE AltName: Full=Keratin-8;
DE Short=K8;
GN Name=krt8 {ECO:0000312|EMBL:AAH67610.1}; Synonyms=krt2-8;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1] {ECO:0000312|EMBL:AAQ97976.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney marrow {ECO:0000312|EMBL:AAQ97976.1};
RX PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y.,
RA Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X.,
RA Look A.T., Chen Z.;
RT "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN [2] {ECO:0000312|EMBL:AAH67610.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney {ECO:0000312|EMBL:AAH67610.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305, ECO:0000312|EMBL:CAD31062.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 21-519, TISSUE SPECIFICITY, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=12558605; DOI=10.1046/j.1432-0436.2003.700607.x;
RA Schaffeld M., Knappe M., Hunzinger C., Markl J.;
RT "cDNA sequences of the authentic keratins 8 and 18 in zebrafish.";
RL Differentiation 71:73-82(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18307296; DOI=10.1021/pr700667w;
RA Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J.,
RA Slijper M., Heck A.J.R.;
RT "Online automated in vivo zebrafish phosphoproteomics: from large-scale
RT analysis down to a single embryo.";
RL J. Proteome Res. 7:1555-1564(2008).
CC -!- FUNCTION: Together with KRT19, helps to link the contractile apparatus
CC to dystrophin at the costameres of striated muscle.
CC {ECO:0000250|UniProtKB:P05787}.
CC -!- SUBUNIT: Heterotetramer of two type I and two type II keratins.
CC Keratin-8 associates with keratin-18 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q10758}.
CC Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q10758}. Nucleus matrix
CC {ECO:0000250|UniProtKB:Q10758}.
CC -!- TISSUE SPECIFICITY: Expressed in simple epithelia.
CC {ECO:0000269|PubMed:12558605}.
CC -!- MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar
CC keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
CC -!- SIMILARITY: Belongs to the intermediate filament family.
CC {ECO:0000255|PROSITE-ProRule:PRU01188}.
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DR EMBL; AY423000; AAQ97976.1; -; mRNA.
DR EMBL; BC067610; AAH67610.1; -; mRNA.
DR EMBL; AJ460000; CAD31062.1; -; mRNA.
DR RefSeq; NP_956374.1; NM_200080.2.
DR AlphaFoldDB; Q6NWF6; -.
DR SMR; Q6NWF6; -.
DR IntAct; Q6NWF6; 1.
DR MINT; Q6NWF6; -.
DR STRING; 7955.ENSDARP00000075658; -.
DR iPTMnet; Q6NWF6; -.
DR PaxDb; Q6NWF6; -.
DR PRIDE; Q6NWF6; -.
DR Ensembl; ENSDART00000081215; ENSDARP00000075658; ENSDARG00000058358.
DR GeneID; 797433; -.
DR KEGG; dre:797433; -.
DR CTD; 3856; -.
DR ZFIN; ZDB-GENE-030411-5; krt8.
DR eggNOG; ENOG502QURK; Eukaryota.
DR GeneTree; ENSGT00940000161090; -.
DR HOGENOM; CLU_012560_5_4_1; -.
DR InParanoid; Q6NWF6; -.
DR OMA; IDKIQFP; -.
DR OrthoDB; 824246at2759; -.
DR PhylomeDB; Q6NWF6; -.
DR TreeFam; TF317854; -.
DR PRO; PR:Q6NWF6; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 23.
DR Bgee; ENSDARG00000058358; Expressed in gastrula and 26 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0045095; C:keratin filament; IBA:GO_Central.
DR GO; GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030280; F:structural constituent of skin epidermis; IBA:GO_Central.
DR GO; GO:0045109; P:intermediate filament organization; IBA:GO_Central.
DR GO; GO:0031424; P:keratinization; IBA:GO_Central.
DR InterPro; IPR018039; IF_conserved.
DR InterPro; IPR039008; IF_rod_dom.
DR InterPro; IPR032444; Keratin_2_head.
DR InterPro; IPR003054; Keratin_II.
DR Pfam; PF00038; Filament; 1.
DR Pfam; PF16208; Keratin_2_head; 1.
DR PRINTS; PR01276; TYPE2KERATIN.
DR SMART; SM01391; Filament; 1.
DR PROSITE; PS00226; IF_ROD_1; 1.
DR PROSITE; PS51842; IF_ROD_2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Intermediate filament; Keratin; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..520
FT /note="Keratin, type II cytoskeletal 8"
FT /id="PRO_0000228676"
FT DOMAIN 115..426
FT /note="IF rod"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01188"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2..114
FT /note="Head"
FT /evidence="ECO:0000255"
FT REGION 115..150
FT /note="Coil 1A"
FT /evidence="ECO:0000255"
FT REGION 151..166
FT /note="Linker 1"
FT /evidence="ECO:0000255"
FT REGION 168..259
FT /note="Coil 1B"
FT /evidence="ECO:0000255"
FT REGION 260..283
FT /note="Linker 12"
FT /evidence="ECO:0000255"
FT REGION 284..422
FT /note="Coil 2"
FT /evidence="ECO:0000255"
FT REGION 423..520
FT /note="Tail"
FT /evidence="ECO:0000255"
FT SITE 366
FT /note="Stutter"
FT /evidence="ECO:0000255"
FT MOD_RES 28
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18307296"
SQ SEQUENCE 520 AA; 57760 MW; 0E39BECA0E494181 CRC64;
MSTYSKKTSY TVKSSSSGSI PRNFSSLSYS GPSMSRQSYS ARSSYGGVNR GMGAGMGGGS
GFISSSSAYG LGMGMGTGMG AGVVAPIQAV TVNKSLLAPL NLEIDPNIQI VRTQEKEQIK
TLNNRFASFI DKVRFLEQQN KMLETKWSLL QNQTATRSNI DAMFEAYIAN LRRQLDSLGN
DKMKLEADLH NMQGLVEDFK NKYEDEINKR TECENEFVLI KKDVDEAYMN KVELEAKLES
LTDEINFLRQ IFEEEIRELQ SQIKDTSVVV EMDNSRNLDM DAIVAEVRAQ YEDIANRSRA
EAEMWYKSKY EEMQTSANKY GDDLRSTKTE IADLNRMIQR LQSEIDAVKG QRANLENQIA
EAEERGEMAV RDAKGRIKDL EDALQRAKQD MARQIREYQD LMNVKLALDI EIATYRKLLE
GEEDRLATGI KAINISKQST SYGGYPMESA GSSYSTYSSG YSSGLSGGYG GGYSGGYSGG
YSSGSGYSDT VSQTKKSVVI KMIETKDGRV VSESSEVVQD
