ID   K3_VACCC                Reviewed;          88 AA.
AC   P20639;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Protein K3;
GN   ORFNames=K3L;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
RN   [3]
RP   FUNCTION.
RX   PubMed=1711259; DOI=10.1016/0042-6822(91)90158-8;
RA   Beattie E., Tartaglia J., Paoletti E.;
RT   "Vaccinia virus-encoded eIF-2 alpha homolog abrogates the antiviral effect
RT   of interferon.";
RL   Virology 183:419-422(1991).
CC   -!- FUNCTION: Viral mimic of eIF-2-alpha that acts as a pseudosubstrate for
CC       EIF2AK2/PKR kinase. Inhibits therefore eIF-2-alpha phosphorylation by
CC       host EIF2AK2/PKR kinase and prevents protein synthesis shutoff (By
CC       similarity). {ECO:0000250, ECO:0000269|PubMed:1711259}.
CC   -!- SUBUNIT: Interacts with host PKR kinase. {ECO:0000250}.
CC   -!- INTERACTION:
CC       P20639; P19525: EIF2AK2; Xeno; NbExp=3; IntAct=EBI-8674942, EBI-640775;
CC   -!- SIMILARITY: Belongs to the poxviridae K3 protein family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M35027; AAA48009.1; -; Genomic_DNA.
DR   PIR; B42505; B42505.
DR   SMR; P20639; -.
DR   DIP; DIP-57736N; -.
DR   IntAct; P20639; 1.
DR   MINT; P20639; -.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0030414; F:peptidase inhibitor activity; IEA:InterPro.
DR   GO; GO:0030291; F:protein serine/threonine kinase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0039580; P:suppression by virus of host PKR signaling; IEA:UniProtKB-KW.
DR   GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR022967; S1_dom.
DR   InterPro; IPR003029; S1_domain.
DR   InterPro; IPR016397; Serpin_prd_poxvir.
DR   Pfam; PF00575; S1; 1.
DR   PIRSF; PIRSF003760; VAC_K3L_Serpin_prd; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Inhibition of host innate immune response by virus;
KW   Inhibition of host interferon signaling pathway by virus;
KW   Inhibition of host PKR by virus; Reference proteome; RNA-binding;
KW   Viral immunoevasion.
FT   CHAIN           1..88
FT                   /note="Protein K3"
FT                   /id="PRO_0000099598"
FT   DOMAIN          8..82
FT                   /note="S1 motif"
SQ   SEQUENCE   88 AA;  10496 MW;  196AD69CD94AE4F0 CRC64;
     MLAFCYSLPN AGDVIKGRVY EKDYALYIYL FDYPHSEAIL AESVKMHMDR YVEYRDKLVG
     KTVKVKVIRV DYTKGYIDVN YKRMCRHQ