ID   K4_VACCW                Reviewed;         424 AA.
AC   P18377; Q76ZX6;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Phospholipase-D-like protein K4;
DE   AltName: Full=Protein K3;
GN   OrderedLocusNames=VACWR035; ORFNames=K3;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3264331; DOI=10.1099/0022-1317-69-12-2995;
RA   Boursnell M.E.G., Foulds I.J., Campbell J.I., Binns M.M.;
RT   "Non-essential genes in the vaccinia virus HindIII K fragment: a gene
RT   related to serine protease inhibitors and a gene related to the 37K
RT   vaccinia virus major envelope antigen.";
RL   J. Gen. Virol. 69:2995-3003(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA nicking enzyme that cleaves extruded cruciform DNA at its
CC       apex (By similarity). Probably nicks viral hairpins. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=Virion core.
CC       {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D00382; BAA00289.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89314.1; -; Genomic_DNA.
DR   PIR; JS0213; WMVZK3.
DR   RefSeq; YP_232917.1; NC_006998.1.
DR   SMR; P18377; -.
DR   DNASU; 3707650; -.
DR   GeneID; 3707650; -.
DR   KEGG; vg:3707650; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR032803; PLDc_3.
DR   InterPro; IPR001736; PLipase_D/transphosphatidylase.
DR   Pfam; PF00614; PLDc; 2.
DR   Pfam; PF13918; PLDc_3; 1.
DR   SMART; SM00155; PLDc; 2.
DR   PROSITE; PS50035; PLD; 2.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Reference proteome; Repeat; Virion.
FT   CHAIN           1..424
FT                   /note="Phospholipase-D-like protein K4"
FT                   /id="PRO_0000099602"
FT   DOMAIN          110..137
FT                   /note="PLD phosphodiesterase 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00153"
FT   DOMAIN          320..346
FT                   /note="PLD phosphodiesterase 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00153"
SQ   SEQUENCE   424 AA;  48873 MW;  58394176A39297A5 CRC64;
     MNPDNTIAVI TETIPIGMQF DKVYLSTFNM WREILSNTTK TLDISSFYWS LSDEVGTNFG
     TIILNKIVQL PKRGVRVRVA VNKSNKPLKD VERLQMAGVE VRYIDITNIL GGVLHTKFWI
     SDNTHIYLGS ANMDWRSLTQ VKELGIAIFN NRNLAADLTQ IFEVYWYLGV NNLPYNWKNF
     YPSYYNTDHP LSINVSGVPH SVFIASAPQQ LCTMERTNDL TALLSCIRNA SKFVYVSVMN
     FIPIIYSKAG NILFWPYIED ELRRAAIDRQ VSVKLLISCW QRSSFIMRNF LRSIAMLKSK
     NINIEVKLFI VPDADPPIPY SRVNHAKYMV TDKTAYIGTS NWTGNYFTDT CGASINITPD
     DGLGLRQQLE DIFMRDWNSK YSYELYDTSP TKRCRLLKNM KQCTNDIYCD EIQPEKEIPE
     YSLE