K6PF_THELI
ID K6PF_THELI Reviewed; 459 AA.
AC Q977Q3;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=ADP-specific phosphofructokinase {ECO:0000255|HAMAP-Rule:MF_00561};
DE EC=2.7.1.146 {ECO:0000255|HAMAP-Rule:MF_00561};
DE AltName: Full=ADP-dependent phosphofructokinase {ECO:0000255|HAMAP-Rule:MF_00561};
DE Short=ADP-Pfk {ECO:0000255|HAMAP-Rule:MF_00561};
GN Name=pfkC {ECO:0000255|HAMAP-Rule:MF_00561}; Synonyms=pfk;
OS Thermococcus litoralis.
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=2265;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Jeong J., Ito S., Fushinobu S., Wakagi T.;
RT "Molecular characterization of ADP-dependent phosphofructokinase from a
RT hyperthermophile.";
RL Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the phosphorylation of fructose 6-phosphate to
CC fructose 1,6-bisphosphate using ADP as the phosphate donor.
CC {ECO:0000255|HAMAP-Rule:MF_00561}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ADP + beta-D-fructose 6-phosphate = AMP + beta-D-fructose 1,6-
CC bisphosphate + H(+); Xref=Rhea:RHEA:20105, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:32966, ChEBI:CHEBI:57634, ChEBI:CHEBI:456215,
CC ChEBI:CHEBI:456216; EC=2.7.1.146; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00561};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00561};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00561};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis. {ECO:0000255|HAMAP-
CC Rule:MF_00561}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00561}.
CC -!- SIMILARITY: Belongs to the carbohydrate kinase PfkC family.
CC {ECO:0000255|HAMAP-Rule:MF_00561}.
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DR EMBL; AB050016; BAB69952.1; -; Genomic_DNA.
DR RefSeq; WP_020953560.1; NC_022084.1.
DR AlphaFoldDB; Q977Q3; -.
DR SMR; Q977Q3; -.
DR GeneID; 16548417; -.
DR OMA; NRIFEFR; -.
DR BRENDA; 2.7.1.146; 6302.
DR UniPathway; UPA00109; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0043844; F:ADP-specific phosphofructokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0008443; F:phosphofructokinase activity; IEA:InterPro.
DR GO; GO:0006000; P:fructose metabolic process; IEA:InterPro.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1190.20; -; 1.
DR HAMAP; MF_00561; ADP_PFKinase; 1.
DR InterPro; IPR007666; ADP_PFK/GK.
DR InterPro; IPR015990; ADP_PFK/GK_arc.
DR InterPro; IPR011790; ADP_PFK_arc.
DR InterPro; IPR029056; Ribokinase-like.
DR PANTHER; PTHR21208; PTHR21208; 1.
DR Pfam; PF04587; ADP_PFK_GK; 1.
DR PIRSF; PIRSF015883; ADP-Pfk_glckin; 1.
DR SUPFAM; SSF53613; SSF53613; 1.
DR TIGRFAMs; TIGR02045; P_fruct_ADP; 1.
DR PROSITE; PS51255; ADPK; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Glycolysis; Kinase; Magnesium; Metal-binding; Transferase.
FT CHAIN 1..459
FT /note="ADP-specific phosphofructokinase"
FT /id="PRO_0000184769"
FT DOMAIN 1..457
FT /note="ADPK"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00561"
FT ACT_SITE 441
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00561"
FT BINDING 268
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00561"
FT BINDING 298
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00561"
FT BINDING 441
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00561"
SQ SEQUENCE 459 AA; 52852 MW; 40852F75DB94E987 CRC64;
MMEFLKDFQK MGIYLAYNVN VDAIVYLNEK HIESLIKEFG AENIKKRIDE YPREINEPLD
FVARLIHALK TGKPQAVPLV SYEADKWFNS RFKYDFERIG GQVGVIANLL ANLDFNKVIA
YSPLLGRKQA EMFVNRDNLL YPVVENGKLV LKKPLEAYRE DDPVKINRIF EFRAGTKFKL
GDETIEVPYS GRFIVACRFE DFARIETSPE LKPYLPEIGE MVDGAILSGY QGLRRYYSDG
KDANYYLRKA KEDIKLLKKK KDIKIHVEFA SIQDRELRKK VIYNIFPLVD SVGMDEAEIA
HILSVLGYRE LSDRIFTYNR IEDAVLGAKI LLDELNLEIL QVHTIYYLMY ITHNDNPLTE
EELTKSLEVG TTLAAARAFL GDIKRPEDVK VGLNIPFNEK GEYVKLRFEE AKAKMRTREY
KIVIIPTRLV RNPVSTVGLG DTISAGAFAS YLSLLKRKE
