ID   KA132_ORTSC             Reviewed;          28 AA.
AC   P83244;
DT   28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2003, sequence version 2.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Potassium channel toxin alpha-KTx 13.2;
DE            Short=OsK-2;
DE            Short=OsK2;
OS   Orthochirus scrobiculosus (Central Asian scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Orthochirus.
OX   NCBI_TaxID=6892 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=11527374; DOI=10.1006/bbrc.2001.5492;
RA   Dudina E.E., Korolkova Y.V., Bocharova N.E., Koshelev S.G., Egorov T.A.,
RA   Huys I., Tytgat J., Grishin E.V.;
RT   "OsK2, a new selective inhibitor of Kv1.2 potassium channels purified from
RT   the venom of the scorpion Orthochirus scrobiculosus.";
RL   Biochem. Biophys. Res. Commun. 286:841-847(2001).
CC   -!- FUNCTION: Potent and selective inhibitor of Kv1.2/KCNA2 potassium
CC       channels. {ECO:0000269|PubMed:11527374}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=2892.14; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:11527374};
CC   -!- MISCELLANEOUS: Does not block Kv1.1/KCNA1 and Kv1.3/KCNA3 potassium
CC       channels. {ECO:0000305|PubMed:11527374}.
CC   -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC       channel inhibitor family. Alpha-KTx 13 subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P83244; -.
DR   SMR; P83244; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019870; F:potassium channel inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0044361; P:negative regulation of voltage-gated potassium channel activity in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Potassium channel impairing toxin; Secreted; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   PEPTIDE         1..28
FT                   /note="Potassium channel toxin alpha-KTx 13.2"
FT                   /id="PRO_0000044910"
FT   REGION          17..24
FT                   /note="Interaction with Ca(2+)-activated K(+) channels"
FT                   /evidence="ECO:0000255"
FT   SITE            18
FT                   /note="Basic residue of the functional dyad"
FT                   /evidence="ECO:0000250"
FT   SITE            27
FT                   /note="Aromatic residue of the functional dyad"
FT                   /evidence="ECO:0000250"
FT   DISULFID        2..19
FT                   /evidence="ECO:0000250"
FT   DISULFID        6..24
FT                   /evidence="ECO:0000250"
FT   DISULFID        10..26
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   28 AA;  2901 MW;  A9BD3332061F99E5 CRC64;
     ACGPGCSGSC RQKGDRIKCI NGSCHCYP