ID   KA221_MESMA             Reviewed;          62 AA.
AC   Q8MUB1; H2ETQ9;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 2.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Potassium channel toxin alpha-KTx 22.1;
DE   AltName: Full=Neurotoxin BmK38;
DE   AltName: Full=Toxin Kcugx;
DE   Flags: Precursor;
OS   Mesobuthus martensii (Manchurian scorpion) (Buthus martensii).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX   NCBI_TaxID=34649;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND NOMENCLATURE.
RC   TISSUE=Venom gland;
RX   PubMed=22230549; DOI=10.1016/j.peptides.2011.12.012;
RA   Zeng X.C., Zhang L., Nie Y., Luo X.;
RT   "Identification and molecular characterization of three new K(+)-channel
RT   specific toxins from the Chinese scorpion Mesobuthus martensii Karsch
RT   revealing intronic number polymorphism and alternative splicing in
RT   duplicated genes.";
RL   Peptides 34:311-323(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Xu C.-Q., Brone B., Chi C.-W.;
RT   "BmK38, a novel scorpion toxin active on potassium channel.";
RL   Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May block potassium channels.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC       channel inhibitor family. Alpha-KTx 22 subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; JQ023127; AEX92698.1; -; Genomic_DNA.
DR   EMBL; JQ074236; AEX92704.1; -; mRNA.
DR   EMBL; AF529081; AAM94410.1; -; mRNA.
DR   AlphaFoldDB; Q8MUB1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Potassium channel impairing toxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..62
FT                   /note="Potassium channel toxin alpha-KTx 22.1"
FT                   /id="PRO_0000227818"
FT   SITE            45
FT                   /note="Basic residue of the functional dyad"
FT                   /evidence="ECO:0000250"
FT   SITE            62
FT                   /note="Aromatic residue of the functional dyad"
FT                   /evidence="ECO:0000250"
FT   DISULFID        28..46
FT                   /evidence="ECO:0000255"
FT   DISULFID        33..59
FT                   /evidence="ECO:0000255"
FT   DISULFID        37..61
FT                   /evidence="ECO:0000255"
FT   CONFLICT        23
FT                   /note="R -> K (in Ref. 2; AAM94410)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        34
FT                   /note="E -> Q (in Ref. 2; AAM94410)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   62 AA;  7139 MW;  34CBE5D359C518FA CRC64;
     MQKLFIVFVL FCILRLDAEV DGRTATFCTQ SICEESCKRQ NKNGRCVIEA EGSLIYHLCK
     CY