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KAD7_MOUSE
ID   KAD7_MOUSE              Reviewed;         614 AA.
AC   Q9D2H2; Q8BVH3;
DT   03-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 147.
DE   RecName: Full=Adenylate kinase 7;
DE            Short=AK 7;
DE            EC=2.7.4.3 {ECO:0000250|UniProtKB:Q96M32};
DE            EC=2.7.4.6 {ECO:0000250|UniProtKB:Q96M32};
DE   AltName: Full=ATP-AMP transphosphorylase 7;
GN   Name=Ak7;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   STRAIN=C57BL/6J; TISSUE=Olfactory bulb, and Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   DISRUPTION PHENOTYPE, AND FUNCTION.
RX   PubMed=18776131; DOI=10.1165/rcmb.2008-0102oc;
RA   Fernandez-Gonzalez A., Kourembanas S., Wyatt T.A., Mitsialis S.A.;
RT   "Mutation of murine adenylate kinase 7 underlies a primary ciliary
RT   dyskinesia phenotype.";
RL   Am. J. Respir. Cell Mol. Biol. 40:305-313(2009).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [4]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=29365104; DOI=10.1093/hmg/ddy034;
RA   Lores P., Coutton C., El Khouri E., Stouvenel L., Givelet M., Thomas L.,
RA   Rode B., Schmitt A., Louis B., Sakheli Z., Chaudhry M.,
RA   Fernandez-Gonzales A., Mitsialis A., Dacheux D., Wolf J.P., Papon J.F.,
RA   Gacon G., Escudier E., Arnoult C., Bonhivers M., Savinov S.N., Amselem S.,
RA   Ray P.F., Dulioust E., Toure A.;
RT   "Homozygous missense mutation L673P in adenylate kinase 7 (AK7) leads to
RT   primary male infertility and multiple morphological anomalies of the
RT   flagella but not to primary ciliary dyskinesia.";
RL   Hum. Mol. Genet. 27:1196-1211(2018).
CC   -!- FUNCTION: Nucleoside monophosphate (NMP) kinase that catalyzes the
CC       reversible transfer of the terminal phosphate group between nucleoside
CC       triphosphates and monophosphates. Has highest activity toward AMP, and
CC       weaker activity toward dAMP, CMP and dCMP. Also displays broad
CC       nucleoside diphosphate kinase activity. Involved in maintaining ciliary
CC       structure and function. {ECO:0000269|PubMed:18776131}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP + ATP = 2 ADP; Xref=Rhea:RHEA:12973, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:456215, ChEBI:CHEBI:456216; EC=2.7.4.3;
CC         Evidence={ECO:0000250|UniProtKB:Q96M32};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-
CC         deoxyribonucleoside 5'-triphosphate + ADP; Xref=Rhea:RHEA:44640,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61560, ChEBI:CHEBI:73316,
CC         ChEBI:CHEBI:456216; EC=2.7.4.6;
CC         Evidence={ECO:0000250|UniProtKB:Q96M32};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-
CC         triphosphate + ADP; Xref=Rhea:RHEA:18113, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57930, ChEBI:CHEBI:61557, ChEBI:CHEBI:456216; EC=2.7.4.6;
CC         Evidence={ECO:0000250|UniProtKB:Q96M32};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q96M32}. Cell projection, cilium, flagellum
CC       {ECO:0000250|UniProtKB:Q96M32}. Note=Detected along the full length of
CC       sperm flagellum, where it colocalizes with alpha-tubulin.
CC       {ECO:0000250|UniProtKB:Q96M32}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9D2H2-2; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9D2H2-1; Sequence=VSP_008474, VSP_008475, VSP_008476;
CC   -!- DISRUPTION PHENOTYPE: Mutant mice present pathological signs
CC       characteristic of primary ciliary dyskinesia (PCD), including high
CC       prevalence of microtubular defects, significantly decreased ciliary
CC       beat frequency, hydrocephalus, abnormal spermatogenesis, mucus
CC       accumulation in the paranasal passages, and exacerbated respiratory
CC       responses upon allergen challenge. This phenotype arose serendipitously
CC       in the process of generating transgenic mice harboring a heme oxygenase
CC       1 construct, due to the serendipitous disruption of Ak7 locus by the
CC       transgene insertion event (PubMed:18776131). Mutant testes reveal the
CC       absence of flagellum structures, compared with those from control
CC       littermates. In mutant testes, nearly all sperm heads are attached to
CC       cytoplasmic mass and not prolonged by flagellar structures, such as
CC       mitochondrial sheath, fibrous sheath and axoneme. In mutant epididymes,
CC       very few sperm structures are observed. The rare sperm flagellum
CC       observed show an absence of the central pair of microtubules
CC       (PubMed:29365104). {ECO:0000269|PubMed:18776131,
CC       ECO:0000269|PubMed:29365104}.
CC   -!- SIMILARITY: In the central section; belongs to the adenylate kinase
CC       family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the dpy-30 family.
CC       {ECO:0000305}.
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DR   EMBL; AK019664; BAB31828.1; -; mRNA.
DR   EMBL; AK078221; BAC37180.1; -; mRNA.
DR   RefSeq; NP_084463.1; NM_030187.1.
DR   AlphaFoldDB; Q9D2H2; -.
DR   SMR; Q9D2H2; -.
DR   BioGRID; 219643; 2.
DR   STRING; 10090.ENSMUSP00000043145; -.
DR   iPTMnet; Q9D2H2; -.
DR   PhosphoSitePlus; Q9D2H2; -.
DR   MaxQB; Q9D2H2; -.
DR   PaxDb; Q9D2H2; -.
DR   PRIDE; Q9D2H2; -.
DR   ProteomicsDB; 269240; -. [Q9D2H2-2]
DR   ProteomicsDB; 269241; -. [Q9D2H2-1]
DR   GeneID; 78801; -.
DR   KEGG; mmu:78801; -.
DR   UCSC; uc007oyu.1; mouse. [Q9D2H2-1]
DR   CTD; 122481; -.
DR   MGI; MGI:1926051; Ak7.
DR   eggNOG; KOG3078; Eukaryota.
DR   InParanoid; Q9D2H2; -.
DR   OrthoDB; 572856at2759; -.
DR   PhylomeDB; Q9D2H2; -.
DR   Reactome; R-MMU-499943; Interconversion of nucleotide di- and triphosphates.
DR   BioGRID-ORCS; 78801; 0 hits in 74 CRISPR screens.
DR   ChiTaRS; Ak7; mouse.
DR   PRO; PR:Q9D2H2; -.
DR   Proteomes; UP000000589; Unplaced.
DR   RNAct; Q9D2H2; protein.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:GOC.
DR   GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR   GO; GO:0004017; F:adenylate kinase activity; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004127; F:cytidylate kinase activity; ISS:UniProtKB.
DR   GO; GO:0004550; F:nucleoside diphosphate kinase activity; ISS:UniProtKB.
DR   GO; GO:0035082; P:axoneme assembly; IMP:MGI.
DR   GO; GO:0007420; P:brain development; IMP:MGI.
DR   GO; GO:0003351; P:epithelial cilium movement involved in extracellular fluid movement; IMP:MGI.
DR   GO; GO:0051649; P:establishment of localization in cell; IMP:MGI.
DR   GO; GO:0002437; P:inflammatory response to antigenic stimulus; IMP:MGI.
DR   GO; GO:0006165; P:nucleoside diphosphate phosphorylation; ISS:UniProtKB.
DR   GO; GO:0009142; P:nucleoside triphosphate biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR   CDD; cd01428; ADK; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR000850; Adenylat/UMP-CMP_kin.
DR   InterPro; IPR026867; AK7.
DR   InterPro; IPR007858; Dpy-30_motif.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR23359; PTHR23359; 1.
DR   PANTHER; PTHR23359:SF105; PTHR23359:SF105; 1.
DR   Pfam; PF05186; Dpy-30; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; ATP-binding; Cell projection; Cilium;
KW   Cilium biogenesis/degradation; Coiled coil; Cytoplasm; Flagellum; Kinase;
KW   Nucleotide-binding; Reference proteome; Transferase.
FT   CHAIN           1..614
FT                   /note="Adenylate kinase 7"
FT                   /id="PRO_0000158954"
FT   REGION          258..503
FT                   /note="Adenylate kinase"
FT                   /evidence="ECO:0000250|UniProtKB:Q96M32"
FT   REGION          288..346
FT                   /note="NMP"
FT                   /evidence="ECO:0000250|UniProtKB:P69441"
FT   REGION          308..327
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          428..438
FT                   /note="LID"
FT                   /evidence="ECO:0000250|UniProtKB:P69441"
FT   REGION          570..614
FT                   /note="DPY-30"
FT                   /evidence="ECO:0000250|UniProtKB:Q96M32"
FT   COILED          376..568
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        311..327
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         268..273
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P69441"
FT   BINDING         323..346
FT                   /ligand="AMP"
FT                   /ligand_id="ChEBI:CHEBI:456215"
FT                   /evidence="ECO:0000250|UniProtKB:P69441"
FT   BINDING         373..376
FT                   /ligand="AMP"
FT                   /ligand_id="ChEBI:CHEBI:456215"
FT                   /evidence="ECO:0000250|UniProtKB:P69441"
FT   BINDING         380
FT                   /ligand="AMP"
FT                   /ligand_id="ChEBI:CHEBI:456215"
FT                   /evidence="ECO:0000250|UniProtKB:P69441"
FT   BINDING         446
FT                   /ligand="AMP"
FT                   /ligand_id="ChEBI:CHEBI:456215"
FT                   /evidence="ECO:0000250|UniProtKB:P69441"
FT   BINDING         478
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:P69441"
FT   VAR_SEQ         1..57
FT                   /note="MECDAVIYNITENVQQVEEALWAVSALNEEISHFEKRKVFILLSTVMTWARS
FT                   KPLDP -> M (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_008474"
FT   VAR_SEQ         181..206
FT                   /note="KCISKNTGPGKIQKVPKENAFLTKDL -> KVCVLTPCRASLSSCTLWMLVT
FT                   DFLC (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_008475"
FT   VAR_SEQ         207..614
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_008476"
SQ   SEQUENCE   614 AA;  70675 MW;  0ABA0DC6C7CE5E8B CRC64;
     MECDAVIYNI TENVQQVEEA LWAVSALNEE ISHFEKRKVF ILLSTVMTWA RSKPLDPDDN
     EVPFTEEDYR RRKHHPNFLD HINAEKIVLK FGKNAKKFAT YVVASGLQYG AEGGILHTFF
     KMAWLGEVPA LPVFGDGTNC IPAIHVVDLA GVIQNIIDHV PKLHYLVAVD EAVHTLEDLV
     KCISKNTGPG KIQKVPKENA FLTKDLTQEY LDHLLVNLRM EALFVKENFN IRWVAQTGFV
     ENINSILKEY KQSRGLLPIK ICILGPPAVG KSSISEELAK YYKLHHIKMK DVIAEAIAKL
     EAIVAPKDSV EGEEEGEEEE EEENVDDAQE LLDGIKESME QNAGRLEDQY IIRFVKEKLK
     SMPCRNQGFI LDGFPKTYDQ AKDLFNQEEE EEEEEIRGKI FPYDKLITPE FVCGLDASDE
     FLKERVMNLP ESVVAGTHYS QDRFLRSLSH YRDINTDDET VFNYFDELEI HPIHIDVGKL
     EDAQNRLAIK QLIKEIGKPR NYGLTDEEKA EEEKKAAEER LAKEAAQTAE LEHKEAMEMA
     EKIARWEEWN KRLEEVKREE RELLEVQSVP LRNYLMTYVM PTLMQGLNEC CKVRPEDPVD
     FLAEYLFKNN PEMQ
 
 
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