KAO_ORYSJ
ID KAO_ORYSJ Reviewed; 506 AA.
AC Q5VRM7; D0PQ37;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Ent-kaurenoic acid oxidase {ECO:0000305};
DE Short=OsKAO {ECO:0000303|PubMed:15075394};
DE EC=1.14.14.107 {ECO:0000250|UniProtKB:O23051};
DE AltName: Full=Cytochrome P450 88A5 {ECO:0000305};
DE AltName: Full=Protein REDUCED POLLEN ELONGATION 1 {ECO:0000303|PubMed:18083909};
GN Name=KAO {ECO:0000312|EMBL:ABY49048.1};
GN Synonyms=CYP88A5 {ECO:0000305}, RPE1 {ECO:0000303|PubMed:18083909};
GN OrderedLocusNames=Os06g0110000 {ECO:0000312|EMBL:BAF18493.1},
GN LOC_Os06g02019 {ECO:0000305};
GN ORFNames=OSJNBa0004I20.18 {ECO:0000312|EMBL:BAD67898.1},
GN P0514G12.42 {ECO:0000312|EMBL:BAD67695.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 128-478.
RX PubMed=19689796; DOI=10.1186/1471-2148-9-206;
RA Yang Y.H., Zhang F.M., Ge S.;
RT "Evolutionary rate patterns of the gibberellin pathway genes.";
RL BMC Evol. Biol. 9:206-206(2009).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=15075394; DOI=10.1104/pp.103.033696;
RA Sakamoto T., Miura K., Itoh H., Tatsumi T., Ueguchi-Tanaka M., Ishiyama K.,
RA Kobayashi M., Agrawal G.K., Takeda S., Abe K., Miyao A., Hirochika H.,
RA Kitano H., Ashikari M., Matsuoka M.;
RT "An overview of gibberellin metabolism enzyme genes and their related
RT mutants in rice.";
RL Plant Physiol. 134:1642-1653(2004).
RN [6]
RP FUNCTION.
RX PubMed=18083909; DOI=10.1105/tpc.107.054759;
RA Chhun T., Aya K., Asano K., Yamamoto E., Morinaka Y., Watanabe M.,
RA Kitano H., Ashikari M., Matsuoka M., Ueguchi-Tanaka M.;
RT "Gibberellin regulates pollen viability and pollen tube growth in rice.";
RL Plant Cell 19:3876-3888(2007).
CC -!- FUNCTION: Involved in gibberellin (GA) biosynthesis. Catalyzes three
CC successive oxidations of ent-kaurenoic acid giving gibberellin 12
CC (GA12), a key step in GAs biosynthesis. GAs, which are involved many
CC processes, including stem elongation, play a central role in plant
CC development (Probable). Required for pollen germination and elongation
CC (PubMed:18083909). {ECO:0000269|PubMed:18083909,
CC ECO:0000305|PubMed:15075394}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ent-kaur-16-en-19-oate + 3 O2 + 3 reduced [NADPH--hemoprotein
CC reductase] = gibberellin A12 + 4 H(+) + 4 H2O + 3 oxidized [NADPH--
CC hemoprotein reductase]; Xref=Rhea:RHEA:33219, Rhea:RHEA-COMP:11964,
CC Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:57297, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:58210, ChEBI:CHEBI:58627; EC=1.14.14.107;
CC Evidence={ECO:0000250|UniProtKB:O23051};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:O23051}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and panicles. Expressed at low
CC levels in vegetative shoot apices, leaf sheaths, leaf blades and stems.
CC {ECO:0000269|PubMed:15075394}.
CC -!- DISRUPTION PHENOTYPE: Severe dwarf phenotype without flower or seed
CC development. {ECO:0000269|PubMed:15075394}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP000616; BAD67695.1; -; Genomic_DNA.
DR EMBL; AP002805; BAD67898.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF18493.1; -; Genomic_DNA.
DR EMBL; AP014962; BAS95781.1; -; Genomic_DNA.
DR EMBL; EU179422; ABY49048.1; -; Genomic_DNA.
DR RefSeq; XP_015643774.1; XM_015788288.1.
DR AlphaFoldDB; Q5VRM7; -.
DR SMR; Q5VRM7; -.
DR STRING; 4530.OS06T0110000-01; -.
DR PaxDb; Q5VRM7; -.
DR PRIDE; Q5VRM7; -.
DR EnsemblPlants; Os06t0110000-01; Os06t0110000-01; Os06g0110000.
DR GeneID; 4339885; -.
DR Gramene; Os06t0110000-01; Os06t0110000-01; Os06g0110000.
DR KEGG; osa:4339885; -.
DR eggNOG; KOG0157; Eukaryota.
DR HOGENOM; CLU_001570_15_5_1; -.
DR InParanoid; Q5VRM7; -.
DR OMA; EMGWPMV; -.
DR OrthoDB; 825914at2759; -.
DR BRENDA; 1.14.14.107; 4460.
DR PlantReactome; R-OSA-1119557; GA12 biosynthesis.
DR UniPathway; UPA00390; -.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0051777; F:ent-kaurenoate oxidase activity; IDA:UniProtKB.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IBA:GO_Central.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016132; P:brassinosteroid biosynthetic process; IBA:GO_Central.
DR GO; GO:0010268; P:brassinosteroid homeostasis; IBA:GO_Central.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IDA:UniProtKB.
DR GO; GO:0009685; P:gibberellin metabolic process; IC:Gramene.
DR GO; GO:0009846; P:pollen germination; IMP:UniProtKB.
DR GO; GO:0009860; P:pollen tube growth; IMP:UniProtKB.
DR GO; GO:0016125; P:sterol metabolic process; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR002397; Cyt_P450_B.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00359; BP450.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..506
FT /note="Ent-kaurenoic acid oxidase"
FT /id="PRO_0000439011"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 451
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 506 AA; 57008 MW; AE3B82D95633A502 CRC64;
MVMEGMGMAA AWAAGDLWVL AAAVVAGVVL VDAVVRRAHD WVRVAALGAE RRSRLPPGEM
GWPMVGSMWA FLRAFKSGNP DAFIASFIRR FGRTGVYRTF MFSSPTILAV TPEACKQVLM
DDEGFVTGWP KATVTLIGPK SFVNMSYDDH RRIRKLTAAP INGFDALTTY LSFIDQTVVA
SLRRWSSPES GQVEFLTELR RMTFKIIVQI FMSGADDATM EALERSYTDL NYGMRAMAIN
LPGFAYYRAL RARRKLVSVL QGVLDGRRAA AAKGFKRSGA MDMMDRLIEA EDERGRRLAD
DEIVDVLIMY LNAGHESSGH ITMWATVFLQ ENPDIFARAK AEQEEIMRSI PATQNGLTLR
DFKKMHFLSQ VVDETLRCVN ISFVSFRQAT RDIFVNGYLI PKGWKVQLWY RSVHMDDQVY
PDPKMFNPSR WEGPPPKAGT FLPFGLGARL CPGNDLAKLE ISVFLHHFLL GYKLKRANPK
CRVRYLPHPR PVDNCLATIT KVSDEH