ID   KAP3_CRIFA              Reviewed;         134 AA.
AC   Q8IS98;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=kinetoplast-associated protein 3;
DE   AltName: Full=Histone H1-like protein p17;
DE   Flags: Precursor;
GN   Name=KAP3;
OS   Crithidia fasciculata.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Leishmaniinae; Crithidia.
OX   NCBI_TaxID=5656;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RX   PubMed=9297699; DOI=10.1016/s0166-6851(97)00099-6;
RA   Hines J.C., Ray D.S.;
RT   "Tandem arrangement of two genes encoding kinetoplast-associated H1
RT   histone-like proteins.";
RL   Mol. Biochem. Parasitol. 89:41-49(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], DNA-BINDING, FUNCTION, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=8552084; DOI=10.1128/mcb.16.2.564;
RA   Xu C.W., Hines J.C., Engel M.L., Russell D.G., Ray D.S.;
RT   "Nucleus-encoded histone H1-like proteins are associated with kinetoplast
RT   DNA in the trypanosomatid Crithidia fasciculata.";
RL   Mol. Cell. Biol. 16:564-576(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-19, PROTEIN SEQUENCE OF 10-30, DNA-BINDING,
RP   AND SUBCELLULAR LOCATION.
RX   PubMed=8446592; DOI=10.1073/pnas.90.5.1786;
RA   Xu C., Ray D.S.;
RT   "Isolation of proteins associated with kinetoplast DNA networks in vivo.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:1786-1789(1993).
RN   [4]
RP   INDUCTION.
RX   PubMed=12912886; DOI=10.1128/ec.2.4.671-677.2003;
RA   Avliyakulov N.K., Hines J.C., Ray D.S.;
RT   "Sequence elements in both the intergenic space and the 3' untranslated
RT   region of the Crithidia fasciculata KAP3 gene are required for cell cycle
RT   regulation of KAP3 mRNA.";
RL   Eukaryot. Cell 2:671-677(2003).
CC   -!- FUNCTION: Histone H1-like DNA-binding protein involved in the
CC       organization and segregation of kinetoplast DNA (kDNA). The
CC       mitochondrial DNA of kinetoplastid protozoa consists of about 5,000
CC       minicircles and 20 to 30 maxicircles. These circular DNAs are held
CC       together by catenation into a highly organized compact disk structure
CC       referred to as a kinetoplast DNA (kDNA) network. Binds preferentially
CC       to a specific fragment of minicircle DNA and is able to compact kDNA
CC       networks through DNA charge neutralization and condensation.
CC       {ECO:0000269|PubMed:8552084}.
CC   -!- SUBUNIT: Associates with the kinetoplast DNA network.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix, kinetoplast
CC       {ECO:0000269|PubMed:8446592, ECO:0000269|PubMed:8552084}.
CC   -!- INDUCTION: Expression varies periodically during the cell cycle in the
CC       same manner as transcripts of several kinetoplast and nuclear DNA
CC       replication genes. {ECO:0000269|PubMed:12912886,
CC       ECO:0000269|PubMed:9297699}.
CC   -!- SIMILARITY: Belongs to the KAP family. {ECO:0000305}.
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DR   EMBL; AY143553; AAN46297.1; -; Genomic_DNA.
DR   PIR; JC6091; JC6091.
DR   AlphaFoldDB; Q8IS98; -.
DR   SMR; Q8IS98; -.
DR   VEuPathDB; TriTrypDB:CFAC1_300049800; -.
DR   GO; GO:0020023; C:kinetoplast; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR   InterPro; IPR009071; HMG_box_dom.
DR   SMART; SM00398; HMG; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA-binding; Kinetoplast; Mitochondrion.
FT   PROPEP          1..10
FT                   /id="PRO_0000409302"
FT   CHAIN           11..134
FT                   /note="kinetoplast-associated protein 3"
FT                   /id="PRO_0000409303"
FT   REGION          106..134
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   134 AA;  15258 MW;  15CE89868A02A250 CRC64;
     MLRRSPTLLR VSPFSLYMKD LAKNGTLQND RNPAKTASRL YRKLSEPEKM ALQKRAARVS
     YPALDAYNRF QKEYAHRFLH LSNKKRQREV SKLWAELKKN GTVKVPKAPK AAKSASSKVK
     TAAKTAKKTT AARK