KAR1_YEAST
ID KAR1_YEAST Reviewed; 433 AA.
AC P11927; D6W0Z9; E9P8Y4;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Cell division control protein KAR1;
GN Name=KAR1; OrderedLocusNames=YNL188W; ORFNames=N1611;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3030557; DOI=10.1016/0092-8674(87)90712-4;
RA Rose M.D., Fink G.R.;
RT "KAR1, a gene required for function of both intranuclear and extranuclear
RT microtubules in yeast.";
RL Cell 48:1047-1060(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION.
RX PubMed=8070654; DOI=10.1093/genetics/137.2.407;
RA Vallen E.A., Ho W., Winey M., Rose M.D.;
RT "Genetic interactions between CDC31 and KAR1, two genes required for
RT duplication of the microtubule organizing center in Saccharomyces
RT cerevisiae.";
RL Genetics 137:407-422(1994).
RN [6]
RP FUNCTION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7876310; DOI=10.1083/jcb.128.5.863;
RA Spang A., Courtney I., Grein K., Matzner M., Schiebel E.;
RT "The Cdc31p-binding protein Kar1p is a component of the half bridge of the
RT yeast spindle pole body.";
RL J. Cell Biol. 128:863-877(1995).
RN [7]
RP FUNCTION, AND INTERACTION WITH SPC72.
RX PubMed=10428957; DOI=10.1093/emboj/18.15.4180;
RA Pereira G., Grueneberg U., Knop M., Schiebel E.;
RT "Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with
RT Kar1p is essential for microtubule function during karyogamy.";
RL EMBO J. 18:4180-4195(1999).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-233, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: KAR1 is required for function of both intranuclear and
CC extranuclear microtubules. KAR1 helps localize CDC31 to the spindle
CC pole body (SPB), CDC31 then initiates SPB duplication via interaction
CC with a downstream effector. {ECO:0000269|PubMed:10428957,
CC ECO:0000269|PubMed:7876310, ECO:0000269|PubMed:8070654}.
CC -!- SUBUNIT: Interacts with SPC72. {ECO:0000269|PubMed:10428957}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, spindle pole body.
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DR EMBL; M15683; AAA34716.1; -; Genomic_DNA.
DR EMBL; Z71464; CAA96082.1; -; Genomic_DNA.
DR EMBL; AY692882; AAT92901.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10365.1; -; Genomic_DNA.
DR PIR; S63143; S63143.
DR RefSeq; NP_014211.1; NM_001183026.1.
DR PDB; 1OQP; NMR; -; B=239-257.
DR PDBsum; 1OQP; -.
DR AlphaFoldDB; P11927; -.
DR BMRB; P11927; -.
DR SMR; P11927; -.
DR BioGRID; 35645; 30.
DR DIP; DIP-2274N; -.
DR IntAct; P11927; 6.
DR STRING; 4932.YNL188W; -.
DR iPTMnet; P11927; -.
DR MaxQB; P11927; -.
DR PaxDb; P11927; -.
DR PRIDE; P11927; -.
DR EnsemblFungi; YNL188W_mRNA; YNL188W; YNL188W.
DR GeneID; 855533; -.
DR KEGG; sce:YNL188W; -.
DR SGD; S000005132; KAR1.
DR VEuPathDB; FungiDB:YNL188W; -.
DR eggNOG; ENOG502S30N; Eukaryota.
DR HOGENOM; CLU_051895_0_0_1; -.
DR InParanoid; P11927; -.
DR OMA; LIESKWH; -.
DR BioCyc; YEAST:G3O-33199-MON; -.
DR EvolutionaryTrace; P11927; -.
DR PRO; PR:P11927; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P11927; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005825; C:half bridge of spindle pole body; IDA:SGD.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IMP:SGD.
DR GO; GO:0030474; P:spindle pole body duplication; IMP:SGD.
DR IDEAL; IID50070; -.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Phosphoprotein; Reference proteome.
FT CHAIN 1..433
FT /note="Cell division control protein KAR1"
FT /id="PRO_0000084295"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 69..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 207..227
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 233
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CONFLICT 95
FT /note="Y -> H (in Ref. 4; AAT92901)"
FT /evidence="ECO:0000305"
FT CONFLICT 199
FT /note="A -> V (in Ref. 1; AAA34716)"
FT /evidence="ECO:0000305"
FT HELIX 240..252
FT /evidence="ECO:0007829|PDB:1OQP"
SQ SEQUENCE 433 AA; 50653 MW; D65331B3B2B068E4 CRC64;
MNVTSPKDGN HSFSKKNRFN TNKPRFHKLN EQAQSINLPE DRDSIVSSNT TSIMTDDAFD
YNEGIASRTK NINSDSDRSN DTIKQNNYNK RETGYNPFYN GSGINQRYTQ FRKREFEPTL
AENKAEEYIS DEDNVKIDED NIENELQFTP KIKEASILRS SLLGQRNVLN TRNPKSKESH
IKVKPIINNK SSSQRKSSAA LRKQLGKPLP LPYLNSPNSD STPTLQRKEE VFTDEVLQKK
RELIESKWHR LLFHDKKMVE KKLESLREYE RKRMPPRGTD VSSSEQDNSF KISTPTKSYV
SLEQKPLPNL SAMNNFNDVT DNKEKEETNN NILKFQAQRD PLQILQSEIE MHTKKLDTII
ELLKDDTDSK EKRKVVTNDN AAPEQMVNKG WRKNVMMIYK KSGNIMKKYR EYFLWTICIL
ILLYCNIYVY YRF
