ID   KAR4_YEAST              Reviewed;         335 AA.
AC   P25583; D6VQW2;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   03-AUG-2022, entry version 160.
DE   RecName: Full=Karyogamy protein KAR4;
GN   Name=KAR4; OrderedLocusNames=YCL055W; ORFNames=YCL432, YCL55W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1441748; DOI=10.1002/yea.320080813;
RA   Defoor E., Debrabandere R., Keyers B., Voet M., Volckaert G.;
RT   "Nucleotide sequence of D10B, a BamHI fragment on the small-ring chromosome
RT   III of Saccharomyces cerevisiae.";
RL   Yeast 8:681-687(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=1574125; DOI=10.1038/357038a0;
RA   Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA   Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA   Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA   Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA   Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA   Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA   Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA   Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA   Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA   Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA   Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA   Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA   Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA   Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA   Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA   Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA   Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA   Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA   Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA   Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA   Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA   Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA   Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA   Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA   Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA   Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA   Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA   Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA   Sgouros J.G.;
RT   "The complete DNA sequence of yeast chromosome III.";
RL   Nature 357:38-46(1992).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=8754797; DOI=10.1128/mcb.16.8.3990;
RA   Kurihara L.J., Stewart B.G., Gammie A.E., Rose M.D.;
RT   "Kar4p, a karyogamy-specific component of the yeast pheromone response
RT   pathway.";
RL   Mol. Cell. Biol. 16:3990-4002(1996).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: May assist STE12 in the pheromone-dependent expression of
CC       KAR3 and CIK1. Also required for meiosis.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: Present with 1690 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the MT-A70-like family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00489}.
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DR   EMBL; X59720; CAA42390.1; -; Genomic_DNA.
DR   EMBL; BK006937; DAA07431.1; -; Genomic_DNA.
DR   PIR; S19385; S19385.
DR   RefSeq; NP_009876.1; NM_001178699.1.
DR   AlphaFoldDB; P25583; -.
DR   SMR; P25583; -.
DR   BioGRID; 30931; 71.
DR   DIP; DIP-1401N; -.
DR   IntAct; P25583; 7.
DR   MINT; P25583; -.
DR   STRING; 4932.YCL055W; -.
DR   iPTMnet; P25583; -.
DR   MaxQB; P25583; -.
DR   PaxDb; P25583; -.
DR   PRIDE; P25583; -.
DR   EnsemblFungi; YCL055W_mRNA; YCL055W; YCL055W.
DR   GeneID; 850303; -.
DR   KEGG; sce:YCL055W; -.
DR   SGD; S000000560; KAR4.
DR   VEuPathDB; FungiDB:YCL055W; -.
DR   eggNOG; KOG2097; Eukaryota.
DR   GeneTree; ENSGT00550000075003; -.
DR   HOGENOM; CLU_046318_0_0_1; -.
DR   InParanoid; P25583; -.
DR   OMA; NINKPGH; -.
DR   BioCyc; YEAST:G3O-29307-MON; -.
DR   PRO; PR:P25583; -.
DR   Proteomes; UP000002311; Chromosome III.
DR   RNAct; P25583; protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005829; C:cytosol; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0036396; C:RNA N6-methyladenosine methyltransferase complex; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:SGD.
DR   GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR   GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IMP:SGD.
DR   GO; GO:0051321; P:meiotic cell cycle; IMP:SGD.
DR   GO; GO:0080009; P:mRNA methylation; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR   InterPro; IPR045123; METTL14-like.
DR   InterPro; IPR007757; MT-A70-like.
DR   PANTHER; PTHR13107; PTHR13107; 1.
DR   Pfam; PF05063; MT-A70; 1.
DR   PROSITE; PS51143; MT_A70; 1.
PE   1: Evidence at protein level;
KW   Karyogamy; Meiosis; Nucleus; Reference proteome.
FT   CHAIN           1..335
FT                   /note="Karyogamy protein KAR4"
FT                   /id="PRO_0000207635"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   335 AA;  38672 MW;  B54A4E2E6563EC81 CRC64;
     MAFQDPTYDQ NKSRHINNSH LQGPNQETIE MKSKHVSFKP SRDFHTNDYS NNYIHGKSLP
     QQHVTNIENR VDGYPKLQKL FQAKAKQINQ FATTPFGCKI GIDSIVPTLN HWIQNENLTF
     DVVMIGCLTE NQFIYPILTQ LPLDRLISKP GFLFIWANSQ KINELTKLLN NEIWAKKFRR
     SEELVFVPID KKSPFYPGLD QDDETLMEKM QWHCWMCITG TVRRSTDGHL IHCNVDTDLS
     IETKDTTNGA VPSHLYRIAE NFSTATRRLH IIPARTGYET PVKVRPGWVI VSPDVMLDNF
     SPKRYKEEIA NLGSNIPLKN EIELLRPRSP VQKAQ