ID   KAR5_ASHGO              Reviewed;         478 AA.
AC   Q759Y0;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Nuclear fusion protein KAR5;
DE   AltName: Full=Karyogamy protein 5;
DE   Flags: Precursor;
GN   Name=KAR5; OrderedLocusNames=ADR143W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 224; 275 AND 384.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Required for nuclear membrane fusion during karyogamy.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KAR5 family. {ECO:0000305}.
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DR   EMBL; AE016817; AAS52063.2; -; Genomic_DNA.
DR   RefSeq; NP_984239.2; NM_209592.2.
DR   AlphaFoldDB; Q759Y0; -.
DR   SMR; Q759Y0; -.
DR   STRING; 33169.AAS52063; -.
DR   EnsemblFungi; AAS52063; AAS52063; AGOS_ADR143W.
DR   GeneID; 4620400; -.
DR   KEGG; ago:AGOS_ADR143W; -.
DR   eggNOG; ENOG502QVCQ; Eukaryota.
DR   HOGENOM; CLU_042075_0_0_1; -.
DR   InParanoid; Q759Y0; -.
DR   OMA; LSICEFQ; -.
DR   Proteomes; UP000000591; Chromosome IV.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0031301; C:integral component of organelle membrane; IBA:GO_Central.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IBA:GO_Central.
DR   GO; GO:0048288; P:nuclear membrane fusion involved in karyogamy; IEA:InterPro.
DR   InterPro; IPR007292; Nuclear_fusion_Kar5.
DR   PANTHER; PTHR28012; PTHR28012; 1.
DR   Pfam; PF04163; Tht1; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Glycoprotein; Karyogamy; Membrane; Nucleus;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..478
FT                   /note="Nuclear fusion protein KAR5"
FT                   /id="PRO_0000308769"
FT   TOPO_DOM        20..419
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        420..442
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        443..454
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        455..477
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        478
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        159
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        371
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        392
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   478 AA;  54034 MW;  2B9B6787D451E159 CRC64;
     MLEILLFLCV IIQRSHINAE ITHVVSHLAE TALRQDTNFQ LLSQDIIAKK FPILDSSCVR
     RALSDFLPQC LQYGFETVPS DVRTQAAVKL SICELQASGV DNMPPECVGA VHFGACLRAM
     ERTPQWWTTY SGNYQHLPST CFENALPYEK EQLLSLFLNI TDVYSNFQDD LVVDLEKYRA
     NFEATVEASL RLMKASLMEG THEIVNQLKD DLNYVNSKLA DMGETITEHT DNVRTVFNDI
     SDELNDYDMA GQIAHLKEDT MSLWQKINSD MGTYHDVQMS SLYNINAVFD TFYNRATESV
     QQVRTSVIES QLETLDLIAD FNSLVRKSIL PVLADELQPQ LQEMSVSISR SLVGLSASYN
     EHLQAWSNRV NETLSEMESH LNNTMSQVEH MNDSIETLEN KVFVLVSLGN ALTTYVKWIY
     TFSRALISGY GIVTLIMSML VVRYSIKLNS SWIKVLGRST FILVAVVLGA RTGSMLSY