KAR5_CANAL
ID KAR5_CANAL Reviewed; 485 AA.
AC Q59WU8; A0A1D8PPH8; A0A1D8PPH9;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Nuclear fusion protein KAR5;
DE AltName: Full=Karyogamy protein 5;
DE Flags: Precursor;
GN Name=KAR5; OrderedLocusNames=CAALFM_C601010WA, CAALFM_C601020WA;
GN ORFNames=CaO19.7750, orf19.103, orf19.104;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- FUNCTION: Required for nuclear membrane fusion during karyogamy.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}. Nucleus membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the KAR5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AOW30047.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AOW30047.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AOW30047.1; Type=Miscellaneous discrepancy; Note=Contains ambiguous sequence between ORF C6_01010W_A and C6_01020W_A. Used assembly 19 sequence to merge the 2 ORFs.; Evidence={ECO:0000305|PubMed:15123810, ECO:0000305|PubMed:24025428};
CC Sequence=AOW30048.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AOW30048.1; Type=Miscellaneous discrepancy; Note=Contains ambiguous sequence between ORF C6_01010W_A and C6_01020W_A. Used assembly 19 sequence to merge the 2 ORFs.; Evidence={ECO:0000305|PubMed:15123810, ECO:0000305|PubMed:24025428};
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DR EMBL; CP017628; AOW30047.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP017628; AOW30048.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_714312.2; XM_709219.2.
DR RefSeq; XP_714313.1; XM_709220.2.
DR AlphaFoldDB; Q59WU8; -.
DR SMR; Q59WU8; -.
DR STRING; 237561.Q59WU8; -.
DR GeneID; 3644049; -.
DR GeneID; 3644050; -.
DR KEGG; cal:CAALFM_C601010WA; -.
DR KEGG; cal:CAALFM_C601020WA; -.
DR CGD; CAL0000183563; KAR5.
DR CGD; CAL0000191239; orf19.104.
DR HOGENOM; CLU_039530_0_0_1; -.
DR InParanoid; Q59WU8; -.
DR OrthoDB; 1476880at2759; -.
DR Proteomes; UP000000559; Chromosome 6.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0031301; C:integral component of organelle membrane; IBA:GO_Central.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IBA:GO_Central.
DR GO; GO:0048288; P:nuclear membrane fusion involved in karyogamy; IEA:InterPro.
DR InterPro; IPR007292; Nuclear_fusion_Kar5.
DR PANTHER; PTHR28012; PTHR28012; 1.
DR Pfam; PF04163; Tht1; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Glycoprotein; Karyogamy; Membrane; Nucleus;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..15
FT /evidence="ECO:0000255"
FT CHAIN 16..485
FT /note="Nuclear fusion protein KAR5"
FT /id="PRO_0000308770"
FT TOPO_DOM 16..423
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 445..450
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 472..485
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 148
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 344
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 370
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 485 AA; 56008 MW; 8C06EE251E03D0C3 CRC64;
MNIVLLIYCL AMVVAHDLQL FGDFKFELSD NWRNDCAKKA LEPIINQCAE GIETISPFQQ
KSIAIQLSIC EFENAEISYP SECRSQNLDT CILLLEKSPQ YWTTFSGYYR EIRNICHQVS
LPFAKDQILQ VYGNITEFYR TLMDEMTNSS KYTENMQNEL KAKFDKLIGV IDLILADREK
NREDLKSSFN MFKNNFEKSL NNALVVMKHS YEDANSNVKE LESHLNYFIN DMSQVYILIN
EKALEVKSQQ DRIKEHNADI LNQIEEIKKN LDNAYEEASE VQISNNQLVH DIQSSLDYSL
FTVSNLNSHL QLSINDFIEK NEDIRSRAPI IFEEIFGLFL NHLNESGQLA MDSFEAALDL
SLNMLHQKLN QTERSIDNLN SKVSDLAHFA DSLKKYASSI FNVPNYVRTS MNHKIQQWRE
FGNIMVVGGV FFFVVLTLLV LSFIRTQVMK VFRFAFIGIP MITGIALAIF ILRLLSMPMK
VVDID
