ID   KAR5_KLULA              Reviewed;         487 AA.
AC   Q6CIJ3;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Nuclear fusion protein KAR5;
DE   AltName: Full=Karyogamy protein 5;
DE   Flags: Precursor;
GN   Name=KAR5; OrderedLocusNames=KLLA0F26180g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for nuclear membrane fusion during karyogamy.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KAR5 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR382126; CAG98954.1; -; Genomic_DNA.
DR   RefSeq; XP_456246.1; XM_456246.1.
DR   AlphaFoldDB; Q6CIJ3; -.
DR   SMR; Q6CIJ3; -.
DR   STRING; 28985.XP_456246.1; -.
DR   EnsemblFungi; CAG98954; CAG98954; KLLA0_F26180g.
DR   GeneID; 2894830; -.
DR   KEGG; kla:KLLA0_F26180g; -.
DR   eggNOG; ENOG502QVCQ; Eukaryota.
DR   HOGENOM; CLU_042075_0_0_1; -.
DR   InParanoid; Q6CIJ3; -.
DR   OMA; LSICEFQ; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IEA:InterPro.
DR   GO; GO:0048288; P:nuclear membrane fusion involved in karyogamy; IEA:InterPro.
DR   InterPro; IPR007292; Nuclear_fusion_Kar5.
DR   PANTHER; PTHR28012; PTHR28012; 1.
DR   Pfam; PF04163; Tht1; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Glycoprotein; Karyogamy; Membrane; Nucleus;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..487
FT                   /note="Nuclear fusion protein KAR5"
FT                   /id="PRO_0000308773"
FT   TOPO_DOM        16..450
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        451..471
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        472..487
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        156
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        229
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        272
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        379
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   487 AA;  56279 MW;  1DC129765CB5E4B5 CRC64;
     MWFLVFSIWI ASGQLLPHIS NVIEKELDQL EITELSREYI NNKFPITQSS CVKNALGEFL
     EICMRKGFEF VDADLRVITA VRLSVCEFES SGLTNYPSEC HEKRLGGIDT ISCVNALESS
     PQWWTTYSGN YQNLPHICME NSLPFEKEQI LELFLNITDM YSEFQRNIEN YWKSFSSDLE
     INGKENIDMI QNLFNSLVND LIQNHKMKDE ELITEFDKMK AEFDIRFFNF TESFDNLNDE
     VNEDLSLIKS HLIETFRQVD SEYMAQLQKN KNSTDKAFNE LESMSTYILD HQKTSMELID
     SFFSDLIDLT RDKNLVISDE LMQTQEETIH LIFQYNKLVH ESVIPLLTDD LLPVVRGVSN
     SIVENLDNMN VELTSHLENV SQTIEVKFKA LEKETDRSLL KAKEVESNLR NLNNLVSTSL
     KGLQTIVRLL TFLLKRQVVL VGILQIFLRK YISMNLYLYA IAVVVTALAG SKVGSWGSLL
     MKSFVTR