ID   KAR5_PICGU              Reviewed;         493 AA.
AC   A5DJU3;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Nuclear fusion protein KAR5;
DE   AltName: Full=Karyogamy protein 5;
DE   Flags: Precursor;
GN   Name=KAR5; ORFNames=PGUG_03544;
OS   Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539
OS   / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Meyerozyma.
OX   NCBI_TaxID=294746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324;
RX   PubMed=19465905; DOI=10.1038/nature08064;
RA   Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA   Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA   Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA   Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA   Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA   Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA   Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA   Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA   Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA   Birren B.W., Kellis M., Cuomo C.A.;
RT   "Evolution of pathogenicity and sexual reproduction in eight Candida
RT   genomes.";
RL   Nature 459:657-662(2009).
CC   -!- FUNCTION: Required for nuclear membrane fusion during karyogamy.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KAR5 family. {ECO:0000305}.
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DR   EMBL; CH408158; EDK39446.2; -; Genomic_DNA.
DR   RefSeq; XP_001484163.1; XM_001484113.1.
DR   AlphaFoldDB; A5DJU3; -.
DR   SMR; A5DJU3; -.
DR   STRING; 4929.XP_001484163.1; -.
DR   EnsemblFungi; EDK39446; EDK39446; PGUG_03544.
DR   GeneID; 5125960; -.
DR   KEGG; pgu:PGUG_03544; -.
DR   VEuPathDB; FungiDB:PGUG_03544; -.
DR   eggNOG; ENOG502QVCQ; Eukaryota.
DR   HOGENOM; CLU_039530_0_0_1; -.
DR   InParanoid; A5DJU3; -.
DR   OMA; LSICEFQ; -.
DR   OrthoDB; 1476880at2759; -.
DR   Proteomes; UP000001997; Unassembled WGS sequence.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IEA:InterPro.
DR   GO; GO:0048288; P:nuclear membrane fusion involved in karyogamy; IEA:InterPro.
DR   InterPro; IPR007292; Nuclear_fusion_Kar5.
DR   PANTHER; PTHR28012; PTHR28012; 1.
DR   Pfam; PF04163; Tht1; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Glycoprotein; Karyogamy; Membrane; Nucleus;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..493
FT                   /note="Nuclear fusion protein KAR5"
FT                   /id="PRO_0000308775"
FT   TOPO_DOM        23..445
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        446..466
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        467..469
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        470..490
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        491..493
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        15
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        145
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        383
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   493 AA;  56885 MW;  4E258FA16B2590A4 CRC64;
     MKKILRLVFI LVYTNISTFV SAHELEDAAS FTADELDRLL EDPPGCIQLA MRPIFEKCVL
     NGIHAVDPKD RRSSAIEMSV CEFESAGVEY PAECSTTDYD TCIWKLGLVP QYWTTFSGNY
     RDIGLFCEGV SRNNEKEQLV LLYSNITKVF AGFRHAFYES YSKSQEMKDE MEEGFSRWSA
     DFDIAKDQHK EFYEFVAKQQ EHIKIELMKN QKVIFDFHDE QEVRFNSYSN HIVDVIDSMA
     VDLDIILAKL ADDGIIEDME NQKSKSLDIM KSYSEDAELT LSRIVSELER VGIIQKNDVS
     IVENLNSGLA DTSNKVSKLN KDFEDLDSHF QHTKDLIESE VSFLFANLIG EMETKLSQAL
     ENVDDRIELH FVSQLEFLDK SLNETWEAIL TFKQDWQIFT SIFENFENIP KSISHFVTSG
     LYKTNEILTQ TSYFWSTILN IPVSLLSNIL RYTMAASWIA ILFILISLRY GSTKSFLLVI
     MVLLVVFLNT HRW