ID   KAR5_YARLI              Reviewed;         407 AA.
AC   Q6C994;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Nuclear fusion protein KAR5;
DE   AltName: Full=Karyogamy protein 5;
DE   Flags: Precursor;
GN   Name=KAR5; OrderedLocusNames=YALI0D12947g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for nuclear membrane fusion during karyogamy.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}. Nucleus membrane
CC       {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KAR5 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR382130; CAG80956.1; -; Genomic_DNA.
DR   RefSeq; XP_502768.1; XM_502768.1.
DR   AlphaFoldDB; Q6C994; -.
DR   SMR; Q6C994; -.
DR   STRING; 4952.CAG80956; -.
DR   EnsemblFungi; CAG80956; CAG80956; YALI0_D12947g.
DR   GeneID; 2911268; -.
DR   KEGG; yli:YALI0D12947g; -.
DR   VEuPathDB; FungiDB:YALI0_D12947g; -.
DR   HOGENOM; CLU_039530_0_0_1; -.
DR   InParanoid; Q6C994; -.
DR   OMA; WISFASH; -.
DR   Proteomes; UP000001300; Chromosome D.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0031301; C:integral component of organelle membrane; IBA:GO_Central.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IBA:GO_Central.
DR   GO; GO:0048288; P:nuclear membrane fusion involved in karyogamy; IEA:InterPro.
DR   InterPro; IPR007292; Nuclear_fusion_Kar5.
DR   PANTHER; PTHR28012; PTHR28012; 1.
DR   Pfam; PF04163; Tht1; 1.
PE   3: Inferred from homology;
KW   Endoplasmic reticulum; Glycoprotein; Karyogamy; Membrane; Nucleus;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..407
FT                   /note="Nuclear fusion protein KAR5"
FT                   /id="PRO_0000308776"
FT   TOPO_DOM        30..382
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        383..403
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        404..407
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        165
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   407 AA;  45815 MW;  5F579D400513EA8B CRC64;
     MSLSYSFNTT IFTENRDLVA ALQPQTNCAR TALTLIVSDC GKLSSFNEDQ QLRVSLAVGL
     AVCEFKAAQV TYPDACNNIE DWMSTSACTQ QLVSSPQWWT TYHGCYNSVK QICHMHEASR
     ECDRALKTHS QIVDMQEKLH TKMDQYWELV ETMSDHRDAV LDYWNDTFDF MSETLAHMKE
     TSVSLNAVYR DNFAQAQEHF QMLSENLQEA RVQMENLGWA AQDAVVSLSK STLAEQSLVS
     ERLKNDASSL HKLLVLAHQD TTESFETQLQ QSLTILVESS DNVLLNHVQQ VSSRLSALMS
     DLEESQKKNM DMQHQLQQKV RTINDDIEGF TDTVKQGLEA SHSLLNLVKS KIQLVNGVVS
     IFSRPVRSAF QLASFIIMIR AAFIGGIYTS IGLVMGSMLG VLVMQQV