ID   KAR5_YEAS7              Reviewed;         504 AA.
AC   A6ZMC4;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Nuclear fusion protein KAR5;
DE   AltName: Full=Karyogamy protein 5;
DE   Flags: Precursor;
GN   Name=KAR5; ORFNames=SCY_4236;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: Required for nuclear membrane fusion during karyogamy.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein. Nucleus membrane; Multi-pass membrane protein.
CC   -!- INDUCTION: By mating pheromone. Expression is directly controlled by
CC       STE12 as cells respond to mating pheromone.
CC   -!- SIMILARITY: Belongs to the KAR5 family. {ECO:0000305}.
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DR   EMBL; AAFW02000020; EDN64454.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6ZMC4; -.
DR   EnsemblFungi; EDN64454; EDN64454; SCY_4236.
DR   HOGENOM; CLU_042075_0_0_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IEA:InterPro.
DR   GO; GO:0048288; P:nuclear membrane fusion involved in karyogamy; IEA:InterPro.
DR   InterPro; IPR007292; Nuclear_fusion_Kar5.
DR   PANTHER; PTHR28012; PTHR28012; 1.
DR   Pfam; PF04163; Tht1; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Karyogamy; Membrane; Nucleus; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..504
FT                   /note="Nuclear fusion protein KAR5"
FT                   /id="PRO_0000308777"
FT   TOPO_DOM        20..452
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        453..470
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        471..481
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        482..502
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        503..504
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        159
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        206
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        313
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        404
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   504 AA;  58382 MW;  868B9D37FC9D4EBD CRC64;
     MFAMRYVYLF AICIKFVSSS ELGKINNLLQ GRLIYTDNSV ATNVLESKFP FLKSTCVKDA
     LKLFLPQCIA NGLESIDAET RVETAIKLSI CEFQASGLGE IPENCMVDDL GSMMDCMFEL
     ESSSQWWTTY SGNYQRLSSI CYENLLPFEK EQILKLFLNI TELYDSFGDD VDTKLNHLMF
     QMEQDSQNFL DDLARMFRNY DNELRNATES NRIILENDLS FFRNKVNDVL YETSEQLEVQ
     IIEKNSQLMN EVDTVHHIMS DLADELAKND IKSKINDLKD DSLNNLQDLV EMSNDVKEYY
     SRNNKLVNTE LENFSMGLKK QLGGMSKDLS ESQMEAIELL QGFNSILHDS LLPSMTDEIV
     PEMTNFKNTL LQEWTAITST LNGDFALWNE EIFSTFNDIS EKLNGTKKKL DDIEIRVSLV
     HKNVMTMMRV LDFMWKTSKM IIRCGYLAVK NKYYWLLCSV VWIWSKYRTS RVNVKMIPIK
     RYYQWAALLL SIYLGAKTGS LIDF