KAR5_YEAS7
ID KAR5_YEAS7 Reviewed; 504 AA.
AC A6ZMC4;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=Nuclear fusion protein KAR5;
DE AltName: Full=Karyogamy protein 5;
DE Flags: Precursor;
GN Name=KAR5; ORFNames=SCY_4236;
OS Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=307796;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YJM789;
RX PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA Steinmetz L.M.;
RT "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT strain YJM789.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC -!- FUNCTION: Required for nuclear membrane fusion during karyogamy.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein. Nucleus membrane; Multi-pass membrane protein.
CC -!- INDUCTION: By mating pheromone. Expression is directly controlled by
CC STE12 as cells respond to mating pheromone.
CC -!- SIMILARITY: Belongs to the KAR5 family. {ECO:0000305}.
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DR EMBL; AAFW02000020; EDN64454.1; -; Genomic_DNA.
DR AlphaFoldDB; A6ZMC4; -.
DR EnsemblFungi; EDN64454; EDN64454; SCY_4236.
DR HOGENOM; CLU_042075_0_0_1; -.
DR Proteomes; UP000007060; Unassembled WGS sequence.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IEA:InterPro.
DR GO; GO:0048288; P:nuclear membrane fusion involved in karyogamy; IEA:InterPro.
DR InterPro; IPR007292; Nuclear_fusion_Kar5.
DR PANTHER; PTHR28012; PTHR28012; 1.
DR Pfam; PF04163; Tht1; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycoprotein; Karyogamy; Membrane; Nucleus; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..504
FT /note="Nuclear fusion protein KAR5"
FT /id="PRO_0000308777"
FT TOPO_DOM 20..452
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 453..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 471..481
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 503..504
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT CARBOHYD 159
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 206
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 313
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 404
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 504 AA; 58382 MW; 868B9D37FC9D4EBD CRC64;
MFAMRYVYLF AICIKFVSSS ELGKINNLLQ GRLIYTDNSV ATNVLESKFP FLKSTCVKDA
LKLFLPQCIA NGLESIDAET RVETAIKLSI CEFQASGLGE IPENCMVDDL GSMMDCMFEL
ESSSQWWTTY SGNYQRLSSI CYENLLPFEK EQILKLFLNI TELYDSFGDD VDTKLNHLMF
QMEQDSQNFL DDLARMFRNY DNELRNATES NRIILENDLS FFRNKVNDVL YETSEQLEVQ
IIEKNSQLMN EVDTVHHIMS DLADELAKND IKSKINDLKD DSLNNLQDLV EMSNDVKEYY
SRNNKLVNTE LENFSMGLKK QLGGMSKDLS ESQMEAIELL QGFNSILHDS LLPSMTDEIV
PEMTNFKNTL LQEWTAITST LNGDFALWNE EIFSTFNDIS EKLNGTKKKL DDIEIRVSLV
HKNVMTMMRV LDFMWKTSKM IIRCGYLAVK NKYYWLLCSV VWIWSKYRTS RVNVKMIPIK
RYYQWAALLL SIYLGAKTGS LIDF