ID   KAR9_YEAST              Reviewed;         644 AA.
AC   P32526; D6W3A1; Q08982;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   03-AUG-2022, entry version 156.
DE   RecName: Full=Karyogamy protein KAR9;
DE   AltName: Full=Cortical protein KAR9;
GN   Name=KAR9; OrderedLocusNames=YPL269W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9442113; DOI=10.1083/jcb.140.2.377;
RA   Miller R.K., Rose M.D.;
RT   "Kar9p is a novel cortical protein required for cytoplasmic microtubule
RT   orientation in yeast.";
RL   J. Cell Biol. 140:377-390(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169875;
RA   Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA   Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA   Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA   Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA   DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA   Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA   Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA   Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA   Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA   Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA   Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA   Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA   Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA   Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA   Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA   Vo D.H., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL   Nature 387:103-105(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 343-644.
RX   PubMed=8395015; DOI=10.1128/mcb.13.9.5861-5876.1993;
RA   Flick J.S., Thorner J.W.;
RT   "Genetic and biochemical characterization of a phosphatidylinositol-
RT   specific phospholipase C in Saccharomyces cerevisiae.";
RL   Mol. Cell. Biol. 13:5861-5876(1993).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-496, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Involved in karyogamy. Component of a cortical adaptor
CC       complex that orients cytoplasmic microtubules. It may be involved in
CC       anchoring cytoplasmic microtubules to the cell cortex.
CC   -!- INTERACTION:
CC       P32526; P19524: MYO2; NbExp=2; IntAct=EBI-9516, EBI-11659;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton. Note=Localizes
CC       to the tip of shmoo projections and to the tip of budding cells in a
CC       cell-cycle dependent manner.
CC   -!- MISCELLANEOUS: Present with 656 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; AF034763; AAB96652.1; -; Genomic_DNA.
DR   EMBL; Z73625; CAA98005.1; -; Genomic_DNA.
DR   EMBL; AY693206; AAT93225.1; -; Genomic_DNA.
DR   EMBL; L13036; AAA99926.1; -; Genomic_DNA.
DR   EMBL; BK006949; DAA11167.1; -; Genomic_DNA.
DR   PIR; S65302; S65302.
DR   RefSeq; NP_015054.1; NM_001184083.1.
DR   PDB; 5N74; X-ray; 2.30 A; I/J/K/L/M/N/O/P=614-633.
DR   PDBsum; 5N74; -.
DR   AlphaFoldDB; P32526; -.
DR   SMR; P32526; -.
DR   BioGRID; 35944; 178.
DR   DIP; DIP-2712N; -.
DR   IntAct; P32526; 7.
DR   MINT; P32526; -.
DR   STRING; 4932.YPL269W; -.
DR   iPTMnet; P32526; -.
DR   MaxQB; P32526; -.
DR   PaxDb; P32526; -.
DR   PRIDE; P32526; -.
DR   EnsemblFungi; YPL269W_mRNA; YPL269W; YPL269W.
DR   GeneID; 855859; -.
DR   KEGG; sce:YPL269W; -.
DR   SGD; S000006190; KAR9.
DR   VEuPathDB; FungiDB:YPL269W; -.
DR   eggNOG; ENOG502QRQ1; Eukaryota.
DR   HOGENOM; CLU_016705_0_0_1; -.
DR   InParanoid; P32526; -.
DR   OMA; ENCTHES; -.
DR   BioCyc; YEAST:G3O-34151-MON; -.
DR   PRO; PR:P32526; -.
DR   Proteomes; UP000002311; Chromosome XVI.
DR   RNAct; P32526; protein.
DR   GO; GO:0005938; C:cell cortex; IDA:SGD.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0005881; C:cytoplasmic microtubule; IDA:SGD.
DR   GO; GO:0000776; C:kinetochore; IDA:SGD.
DR   GO; GO:0043332; C:mating projection tip; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0005816; C:spindle pole body; IDA:SGD.
DR   GO; GO:0051293; P:establishment of spindle localization; IMP:SGD.
DR   GO; GO:0000741; P:karyogamy; IEA:UniProtKB-KW.
DR   GO; GO:0031578; P:mitotic spindle orientation checkpoint signaling; IGI:SGD.
DR   GO; GO:0030473; P:nuclear migration along microtubule; IMP:SGD.
DR   InterPro; IPR013889; Karyogamy_KAR9.
DR   PANTHER; PTHR37271; PTHR37271; 2.
DR   Pfam; PF08580; KAR9; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Cytoskeleton; Karyogamy; Microtubule; Nucleus;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..644
FT                   /note="Karyogamy protein KAR9"
FT                   /id="PRO_0000084296"
FT   REGION          506..534
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          606..644
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        517..534
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        606..626
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         496
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   CONFLICT        641
FT                   /note="T -> A (in Ref. 5; AAA99926)"
FT                   /evidence="ECO:0000305"
FT   TURN            628..630
FT                   /evidence="ECO:0007829|PDB:5N74"
SQ   SEQUENCE   644 AA;  74245 MW;  F14D4D570AC2B5A3 CRC64;
     MDNDGPRSMT IGDDFQENFC ERLERIHNTL HSINDCNSLN ESTTSISETL LVQFYDDLEN
     VASVIPDLVN KKRLGKDDIL LFMDWLLLKK YMLYQFISDV HNIEEGFAHL LDLLEDEFSK
     DDQDSDKYNR FSPMFDVIEE STQIKTQLEP WLTNLKELLD TSLEFNEISK DHMDTLHKII
     NSNISYCLEI QEERFASPIR HTPSFTLEQL VKLLGTHTET TEPKVPKFSP AEDILSRKFL
     NLKKNIPPIE KSLTDILPQR IVQFGHRNIT NITTLQTILQ KKYELIMKDY RFMNSEFREL
     KVELIDKRWN ILFINLNHEL LYILDEIERL QSKLLTTKYT KDITIRLERQ LERKSKTVSK
     TFNIIYRALE FSLLDAGVAS KTNELAQRWL NIKPTADKIL IKSSASNKIA TSKKKIPKPK
     SLGFGRPNSV IGTITQDFQE RVAINEGDSN KTPENSTTVA LKGKKLGKAL LQKMNIKPAT
     SPNSSNAINP FFDPESPNKG KLILSSVPPL PYDETDETTL RVSRGENEKS PDSFITSRHE
     NKVQITETPL MAKNKSVLDI EKDKWNHYRS LPSRIPIYKD KVVKVTVENT PIAKVFQTPP
     TKITTPNSQV WVPSTRRRTR LRPPTPLSQL LSPREGRLDK TPTY