ID   KAS1_STRVN              Reviewed;         421 AA.
AC   P16540;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Granaticin polyketide putative beta-ketoacyl synthase 1;
DE            EC=2.3.1.-;
DE   AltName: Full=ORF1;
GN   Name=gra-orf1;
OS   Streptomyces violaceoruber.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1935;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Tu22;
RX   PubMed=2583128; DOI=10.1002/j.1460-2075.1989.tb08413.x;
RA   Sherman D.H., Malpartida F., Bibb M.J., Kieser H.M., Bibb M.J.,
RA   Hopwood D.A.;
RT   "Structure and deduced function of the granaticin-producing polyketide
RT   synthase gene cluster of Streptomyces violaceoruber Tu22.";
RL   EMBO J. 8:2717-2725(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Tu22;
RX   PubMed=7476861; DOI=10.1007/bf02423457;
RA   Bechthold A., Sohng J.K., Smith T.M., Chu X., Floss H.G.;
RT   "Identification of Streptomyces violaceoruber Tu22 genes involved in the
RT   biosynthesis of granaticin.";
RL   Mol. Gen. Genet. 248:610-620(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Tu22;
RX   PubMed=9831526; DOI=10.1016/s1074-5521(98)90292-7;
RA   Ichinose K., Bedford D.J., Tornus D., Bechthold A., Bibb M.J., Revill W.P.,
RA   Floss H.G., Hopwood D.A.;
RT   "The granaticin biosynthetic gene cluster of Streptomyces violaceoruber
RT   Tu22: sequence analysis and expression in a heterologous host.";
RL   Chem. Biol. 5:647-659(1998).
CC   -!- PATHWAY: Antibiotic biosynthesis; granaticin biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP
CC       synthases family. {ECO:0000305}.
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DR   EMBL; X16300; CAA34369.1; -; Genomic_DNA.
DR   EMBL; X16144; CAA34264.1; -; Genomic_DNA.
DR   EMBL; AJ011500; CAA09653.1; -; Genomic_DNA.
DR   PIR; S05393; S05393.
DR   AlphaFoldDB; P16540; -.
DR   SMR; P16540; -.
DR   UniPathway; UPA00175; -.
DR   GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR   CDD; cd00834; KAS_I_II; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11712; PTHR11712; 1.
DR   Pfam; PF00109; ketoacyl-synt; 1.
DR   Pfam; PF02801; Ketoacyl-synt_C; 1.
DR   SMART; SM00825; PKS_KS; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic biosynthesis; Transferase.
FT   CHAIN           1..421
FT                   /note="Granaticin polyketide putative beta-ketoacyl
FT                   synthase 1"
FT                   /id="PRO_0000180340"
FT   ACT_SITE        169
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
SQ   SEQUENCE   421 AA;  44393 MW;  5012DE3EA5041CD6 CRC64;
     MTRRVVITGV GVRAPGGSGT KEFWDLLTAG RTATRPISFF DASPFRSRIA GEIDFDAVAE
     GFSPREVRRM DRATQFAVAC TRDALADSGL DTGALDPSRI GVALGSAVAS ATSLENEYLV
     MSDSGREWLV DPAHLSPMMF DYLSPGVMPA EVAWAAGAEG PVTMVSDGCT SGLDSVGYAV
     QGTREGSADV VVAGAADTPV SPIVVACFDA IKATTPRNDD PAHASRPFDG TRNGFVLAEG
     AAMFVLEEYE AAQRRGAHIY AEVGGYATRS QAYHMTGLKK DGREMAESIR AALDEARLDR
     TAVDYVNAHG SGTKQNDRHE TAAFKRSLGE HAYAVPVSSI KSMGGHSLGA IGSIEIAASV
     LAIEHNVVPP TANLHTPDPE CDLDYVPLTA REQRVDTVLT VGSGFGGFQS AMVLHRPEEA
     A