ID   KAS2_STRVN              Reviewed;         415 AA.
AC   P16541;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 2.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Granaticin polyketide putative beta-ketoacyl synthase 2;
DE            EC=2.3.1.-;
DE   AltName: Full=ORF2;
GN   Name=gra-orf2;
OS   Streptomyces violaceoruber.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1935;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Tu22;
RX   PubMed=2583128; DOI=10.1002/j.1460-2075.1989.tb08413.x;
RA   Sherman D.H., Malpartida F., Bibb M.J., Kieser H.M., Bibb M.J.,
RA   Hopwood D.A.;
RT   "Structure and deduced function of the granaticin-producing polyketide
RT   synthase gene cluster of Streptomyces violaceoruber Tu22.";
RL   EMBO J. 8:2717-2725(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Tu22;
RX   PubMed=7476861; DOI=10.1007/bf02423457;
RA   Bechthold A., Sohng J.K., Smith T.M., Chu X., Floss H.G.;
RT   "Identification of Streptomyces violaceoruber Tu22 genes involved in the
RT   biosynthesis of granaticin.";
RL   Mol. Gen. Genet. 248:610-620(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Tu22;
RX   PubMed=9831526; DOI=10.1016/s1074-5521(98)90292-7;
RA   Ichinose K., Bedford D.J., Tornus D., Bechthold A., Bibb M.J., Revill W.P.,
RA   Floss H.G., Hopwood D.A.;
RT   "The granaticin biosynthetic gene cluster of Streptomyces violaceoruber
RT   Tu22: sequence analysis and expression in a heterologous host.";
RL   Chem. Biol. 5:647-659(1998).
CC   -!- PATHWAY: Antibiotic biosynthesis; granaticin biosynthesis.
CC   -!- MISCELLANEOUS: This putative ketoacyl synthase lacks the active site
CC       cysteine.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP
CC       synthases family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X16300; CAA34370.1; -; Genomic_DNA.
DR   EMBL; X16144; CAA34265.1; -; Genomic_DNA.
DR   EMBL; AJ011500; CAA09654.1; -; Genomic_DNA.
DR   PIR; S05394; S05394.
DR   AlphaFoldDB; P16541; -.
DR   SMR; P16541; -.
DR   UniPathway; UPA00175; -.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:UniProt.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR000794; Beta-ketoacyl_synthase.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11712; PTHR11712; 1.
DR   Pfam; PF00109; ketoacyl-synt; 1.
DR   Pfam; PF02801; Ketoacyl-synt_C; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic biosynthesis; Transferase.
FT   CHAIN           1..415
FT                   /note="Granaticin polyketide putative beta-ketoacyl
FT                   synthase 2"
FT                   /id="PRO_0000180347"
SQ   SEQUENCE   415 AA;  43402 MW;  285E1405D53A2ADF CRC64;
     MSTPDRRRAV VTGLSVAAPG GLGTERYWKS LLTGENGIAE LSRFDASRYP SRLAGQIDDF
     EASEHLPSRL LPQTDVSTRY ALAAADWALA DAGVGPESGL DDYDLGVVTS TAQGGFDFTH
     REFHKLWSQG PAYVSVYESF AWFYAVNTGQ ISIRNTMRGP SAALVGEQAG GLDAIGHARR
     TVRRGPGWCS AVASTRRSTR GASSSQLSGG LVSTVADPER AYLPFDVDAS GYVPGEGGAV
     LIVEDADSAR ARGAERIYVR SPLRRDPAPG SGRPPALGRA AELALAEAGL TPADISVVFA
     DGAGVPELDR AEADTLARLF GPRGVPVTAP KALTGRLCAG GGPADLAAAL LALRDQVIPA
     TGRHRAVPDA YALDLVTGRP REAALSAALV LARGRHGFNS AVVVTLRGSD HRRPT