KASH5_BOVIN
ID KASH5_BOVIN Reviewed; 525 AA.
AC Q2T9R2; F1N496;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2012, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Protein KASH5 {ECO:0000305};
DE AltName: Full=Coiled-coil domain-containing protein 155;
GN Name=KASH5; Synonyms=CCDC155;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford;
RX PubMed=19393038; DOI=10.1186/gb-2009-10-4-r42;
RA Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., Puiu D.,
RA Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., Marcais G.,
RA Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.;
RT "A whole-genome assembly of the domestic cow, Bos taurus.";
RL Genome Biol. 10:R42.01-R42.10(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: As a component of the LINC (LInker of Nucleoskeleton and
CC Cytoskeleton) complex, involved in the connection between the nuclear
CC lamina and the cytoskeleton. The nucleocytoplasmic interactions
CC established by the LINC complex play an important role in the
CC transmission of mechanical forces across the nuclear envelope and in
CC nuclear movement and positioning. Required for telomere attachment to
CC nuclear envelope in the prophase of meiosis and for rapid telomere
CC prophase movements implicating a SUN1/2:KASH5 LINC complex in which
CC SUN1 and SUN2 seem to act at least partial redundantly. Required for
CC homolog pairing during meiotic prophase in spermatocytes and probably
CC oocytes. Essential for male and female gametogenesis. Recruits
CC cytoplasmic dynein to telomere attachment sites at the nuclear envelope
CC in spermatocytes. In oocytes is involved in meiotic resumption and
CC spindle formation. {ECO:0000250|UniProtKB:Q80VJ8}.
CC -!- SUBUNIT: Core component the LINC complex which is composed of inner
CC nuclear membrane SUN domain-containing proteins coupled to outer
CC nuclear membrane KASH domain-containing nesprins. SUN and KASH domain-
CC containing proteins seem to bind each other promiscuously; however,
CC differentially expression of LINC complex constituents is giving rise
CC to specific assemblies. At least SUN1/2-containing core LINC complexes
CC are proposed to be hexameric composed of three protomers of each KASH
CC and SUN domain-containing protein. Interacts (via the last 22 AA) with
CC SUN1; this interaction mediates its telomere localization by forming a
CC SUN1:KASH5 LINC complex. Component of a probable SUN2:KASH5 LINC
CC complex. Self-associates. Interacts with DYNC1H1, DCTN1, DYNC1I1/2 and
CC PAFAH1B1; suggesting the association with the dynein-dynactin motor
CC complex. {ECO:0000250|UniProtKB:Q80VJ8}.
CC -!- SUBCELLULAR LOCATION: Nucleus outer membrane
CC {ECO:0000250|UniProtKB:Q80VJ8, ECO:0000305}; Single-pass type IV
CC membrane protein {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Nucleus
CC {ECO:0000250|UniProtKB:Q80VJ8}. Chromosome, telomere
CC {ECO:0000250|UniProtKB:Q80VJ8}. Note=Localized exclusively at telomeres
CC from the leptotene to diplotene stages. Colocalizes with SUN2 at sites
CC of telomere attachment in meiocytes. At oocyte MI stage localized
CC around the spindle, at MII stage localized to the spindle poles.
CC {ECO:0000250|UniProtKB:Q80VJ8}.
CC -!- DOMAIN: The C-terminal 22 AA is required and sufficient for
CC localization to telomeres at the nuclear envelope. {ECO:0000250}.
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DR EMBL; DAAA02047474; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC111305; AAI11306.1; -; mRNA.
DR RefSeq; NP_001070414.1; NM_001076946.2.
DR AlphaFoldDB; Q2T9R2; -.
DR SMR; Q2T9R2; -.
DR STRING; 9913.ENSBTAP00000040719; -.
DR PaxDb; Q2T9R2; -.
DR GeneID; 617651; -.
DR KEGG; bta:617651; -.
DR CTD; 147872; -.
DR eggNOG; ENOG502RXNC; Eukaryota.
DR HOGENOM; CLU_039584_1_0_1; -.
DR InParanoid; Q2T9R2; -.
DR OrthoDB; 335596at2759; -.
DR TreeFam; TF337560; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0000781; C:chromosome, telomeric region; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000800; C:lateral element; IBA:GO_Central.
DR GO; GO:0034993; C:meiotic nuclear membrane microtubule tethering complex; IBA:GO_Central.
DR GO; GO:0090619; C:meiotic spindle pole; IBA:GO_Central.
DR GO; GO:0005640; C:nuclear outer membrane; IBA:GO_Central.
DR GO; GO:0070840; F:dynein complex binding; IBA:GO_Central.
DR GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR GO; GO:0090220; P:chromosome localization to nuclear envelope involved in homologous chromosome segregation; IBA:GO_Central.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IBA:GO_Central.
DR GO; GO:0090172; P:microtubule cytoskeleton organization involved in homologous chromosome segregation; IBA:GO_Central.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR GO; GO:0051653; P:spindle localization; IBA:GO_Central.
DR GO; GO:0034397; P:telomere localization; IBA:GO_Central.
DR InterPro; IPR028170; KASH5.
DR InterPro; IPR028168; KASH5_coiled-coil.
DR InterPro; IPR039508; KASH5_EF-hand-like_dom.
DR PANTHER; PTHR47300; PTHR47300; 1.
DR Pfam; PF14658; EF-hand_9; 1.
DR Pfam; PF14662; KASH_CCD; 1.
PE 2: Evidence at transcript level;
KW Chromosome; Coiled coil; Meiosis; Membrane; Nucleus; Reference proteome;
KW Telomere; Transmembrane; Transmembrane helix.
FT CHAIN 1..525
FT /note="Protein KASH5"
FT /id="PRO_0000331526"
FT TOPO_DOM 1..483
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 484..504
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 505..525
FT /note="Perinuclear space"
FT /evidence="ECO:0000255"
FT REGION 127..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 384..409
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 423..444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 164..360
FT /evidence="ECO:0000255"
FT COMPBIAS 389..409
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 324
FT /note="E -> G (in Ref. 2; AAI11306)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 525 AA; 58598 MW; 97C0E856CFA4E57E CRC64;
MDMPEDQAGG PTAKMYLWDQ AEDRSLGTLL SLEEQILNST FEACDPQRTG TVAVTHLLAY
LEAVTGRGPQ DARLQTLACS LDPSGEGPQA TVDLDTFLVV MRDWITACQL DGGLELEEET
AFEGALTSQQ LPSGCPEVED PANLESFGGE DPRPELPATA DLLSSLEDLE LSNRRLAGEN
AKLQRSVETA EEGSARLGEE ISALRKQLRS TQQALQLARG VDEELEDLKT LAKSLEEQNR
SLLAQARHTE KEQQRLVAEM ETLQEENGKL LAERDGVKRR SEELASEKDI LKRQLYECEH
LICQRDAILS ERTRHAESLT KTLEEYRATT QELRLEISHL EEQLSQTQEG LDELSEGAQV
RRVDCTNLLP PSLGVELQAI QQRNLQEESA HPQEGREEPS TRLPRREEED GAEIQVMVDL
PLHPEDSHPG DILGNPPESS PSEPELQQAL VPMVKELVPV RRPVWGQLCL WPLHLRRLRV
TRHLLIPAPL LGLLLLLLLS VLLLGQSPPP TWPHLQLCYL QPPPV
