KATL2_HUMAN
ID KATL2_HUMAN Reviewed; 538 AA.
AC Q8IYT4;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 3.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Katanin p60 ATPase-containing subunit A-like 2 {ECO:0000255|HAMAP-Rule:MF_03025};
DE Short=Katanin p60 subunit A-like 2 {ECO:0000255|HAMAP-Rule:MF_03025};
DE EC=5.6.1.1 {ECO:0000255|HAMAP-Rule:MF_03025};
DE AltName: Full=p60 katanin-like 2 {ECO:0000255|HAMAP-Rule:MF_03025};
GN Name=KATNAL2 {ECO:0000255|HAMAP-Rule:MF_03025};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16177791; DOI=10.1038/nature03983;
RA Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D.,
RA Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J.,
RA Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L.,
RA Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A.,
RA Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C.,
RA Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K.,
RA Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R.,
RA Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 18.";
RL Nature 437:551-555(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ASN-160.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-215 (ISOFORM 1), AND VARIANT ASN-160.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-146 (ISOFORM 1).
RA Arakawa T., Carninci P., Fukuda S., Hasegawa A., Hayashida K., Hori F.,
RA Kai C., Kawai J., Kojima M., Murata M., Nakamura M., Nishiyori H.,
RA Nomura K., Ohno M., Sasaki D., Shibazaki E., Tagami M., Tagami Y.,
RA Hayashizaki Y.;
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=26929214; DOI=10.1074/mcp.m115.056465;
RA Cheung K., Senese S., Kuang J., Bui N., Ongpipattanakul C., Gholkar A.,
RA Cohn W., Capri J., Whitelegge J.P., Torres J.Z.;
RT "Proteomic analysis of the mammalian Katanin family of microtubule-severing
RT enzymes defines Katanin p80 subunit B-like 1 (KATNBL1) as a regulator of
RT mammalian Katanin microtubule-severing.";
RL Mol. Cell. Proteomics 15:1658-1669(2016).
CC -!- FUNCTION: Severs microtubules in vitro in an ATP-dependent manner. This
CC activity may promote rapid reorganization of cellular microtubule
CC arrays. {ECO:0000255|HAMAP-Rule:MF_03025}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1)
CC alpha/beta tubulin heterodimers.; EC=5.6.1.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_03025};
CC -!- INTERACTION:
CC Q8IYT4; P35609: ACTN2; NbExp=3; IntAct=EBI-1044332, EBI-77797;
CC Q8IYT4; Q8TBB1: LNX1; NbExp=3; IntAct=EBI-1044332, EBI-739832;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000255|HAMAP-
CC Rule:MF_03025}. Cytoplasm {ECO:0000269|PubMed:26929214}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:26929214}. Cytoplasm,
CC cytoskeleton, spindle pole {ECO:0000269|PubMed:26929214}.
CC Note=Localizes within the cytoplasm, partially overlapping with
CC microtubules in interphase and to the mitotic spindle and spindle poles
CC during mitosis. {ECO:0000269|PubMed:26929214}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8IYT4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IYT4-2; Sequence=VSP_033523;
CC -!- SIMILARITY: Belongs to the AAA ATPase family. Katanin p60 subunit A1
CC subfamily. A-like 2 sub-subfamily. {ECO:0000255|HAMAP-Rule:MF_03025}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BI560635; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC012254; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC090373; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471096; EAW62904.1; -; Genomic_DNA.
DR EMBL; BC034999; AAH34999.2; -; mRNA.
DR EMBL; BI560635; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; DB461284; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS32828.1; -. [Q8IYT4-2]
DR RefSeq; NP_112593.2; NM_031303.2. [Q8IYT4-2]
DR RefSeq; XP_006722617.1; XM_006722554.3. [Q8IYT4-1]
DR AlphaFoldDB; Q8IYT4; -.
DR SMR; Q8IYT4; -.
DR BioGRID; 123661; 10.
DR ComplexPortal; CPX-6367; Katanin complex, KATNAL2-KATNB1 variant.
DR ComplexPortal; CPX-6383; Katanin complex, KATNAL2-KATNBL1 variant.
DR IntAct; Q8IYT4; 57.
DR STRING; 9606.ENSP00000245121; -.
DR iPTMnet; Q8IYT4; -.
DR PhosphoSitePlus; Q8IYT4; -.
DR BioMuta; KATNAL2; -.
DR DMDM; 189028467; -.
DR EPD; Q8IYT4; -.
DR jPOST; Q8IYT4; -.
DR MassIVE; Q8IYT4; -.
DR MaxQB; Q8IYT4; -.
DR PaxDb; Q8IYT4; -.
DR PeptideAtlas; Q8IYT4; -.
DR PRIDE; Q8IYT4; -.
DR ProteomicsDB; 71235; -. [Q8IYT4-1]
DR ProteomicsDB; 71236; -. [Q8IYT4-2]
DR Antibodypedia; 22528; 66 antibodies from 16 providers.
DR DNASU; 83473; -.
DR Ensembl; ENST00000245121.10; ENSP00000245121.4; ENSG00000167216.19. [Q8IYT4-2]
DR Ensembl; ENST00000356157.12; ENSP00000348478.6; ENSG00000167216.19. [Q8IYT4-1]
DR Ensembl; ENST00000683218.1; ENSP00000508137.1; ENSG00000167216.19. [Q8IYT4-1]
DR GeneID; 83473; -.
DR KEGG; hsa:83473; -.
DR MANE-Select; ENST00000683218.1; ENSP00000508137.1; NM_001387690.1; NP_001374619.1.
DR UCSC; uc002lco.3; human. [Q8IYT4-1]
DR CTD; 83473; -.
DR DisGeNET; 83473; -.
DR GeneCards; KATNAL2; -.
DR HGNC; HGNC:25387; KATNAL2.
DR HPA; ENSG00000167216; Low tissue specificity.
DR MIM; 614697; gene.
DR neXtProt; NX_Q8IYT4; -.
DR OpenTargets; ENSG00000167216; -.
DR PharmGKB; PA134921461; -.
DR VEuPathDB; HostDB:ENSG00000167216; -.
DR eggNOG; KOG0738; Eukaryota.
DR GeneTree; ENSGT00940000157302; -.
DR InParanoid; Q8IYT4; -.
DR OMA; MKTQGKY; -.
DR OrthoDB; 787710at2759; -.
DR PhylomeDB; Q8IYT4; -.
DR TreeFam; TF329610; -.
DR PathwayCommons; Q8IYT4; -.
DR SignaLink; Q8IYT4; -.
DR SIGNOR; Q8IYT4; -.
DR BioGRID-ORCS; 83473; 11 hits in 1067 CRISPR screens.
DR ChiTaRS; KATNAL2; human.
DR GenomeRNAi; 83473; -.
DR Pharos; Q8IYT4; Tbio.
DR PRO; PR:Q8IYT4; -.
DR Proteomes; UP000005640; Chromosome 18.
DR RNAct; Q8IYT4; protein.
DR Bgee; ENSG00000167216; Expressed in right uterine tube and 105 other tissues.
DR ExpressionAtlas; Q8IYT4; baseline and differential.
DR Genevisible; Q8IYT4; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005874; C:microtubule; IDA:UniProtKB.
DR GO; GO:0015630; C:microtubule cytoskeleton; IC:ComplexPortal.
DR GO; GO:0005819; C:spindle; IDA:UniProtKB.
DR GO; GO:0000922; C:spindle pole; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0008017; F:microtubule binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008568; F:microtubule severing ATPase activity; IEA:UniProtKB-EC.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IC:ComplexPortal.
DR GO; GO:0051013; P:microtubule severing; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_03025; Katanin_p60_AL2; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR027497; Katanin_p60_AL2.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF17862; AAA_lid_3; 1.
DR Pfam; PF08513; LisH; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00667; LisH; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50896; LISH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Cytoplasm; Cytoskeleton; Isomerase;
KW Microtubule; Nucleotide-binding; Reference proteome.
FT CHAIN 1..538
FT /note="Katanin p60 ATPase-containing subunit A-like 2"
FT /id="PRO_0000333792"
FT DOMAIN 25..57
FT /note="LisH"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03025"
FT REGION 94..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..139
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 294..301
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03025"
FT VAR_SEQ 1..72
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033523"
FT VARIANT 160
FT /note="S -> N (in dbSNP:rs7233515)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.2"
FT /id="VAR_043160"
FT CONFLICT 179..180
FT /note="AH -> GQ (in Ref. 3; BI560635)"
FT /evidence="ECO:0000305"
FT CONFLICT 207
FT /note="N -> S (in Ref. 3; BI560635)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 538 AA; 61253 MW; F966747821008D3F CRC64;
MELSYQTLKF THQAREACEM RTEARRKNLL ILISHYLTQE GYIDTANALE QETKLGLRRF
EVCDNIDLET ILMEYESYYF VKFQKYPKIV KKSSDTAENN LPQRSRGKTR RMMNDSCQNL
PKINQQRPRS KTTAGKTGDT KSLNKEHPNQ EVVDNTRLES ANFGLHISRI RKDSGEENAH
PRRGQIIDFQ GLLTDAIKGA TSELALNTFD HNPDPSERLL KPLSAFIGMN SEMRELAAVV
SRDIYLHNPN IKWNDIIGLD AAKQLVKEAV VYPIRYPQLF TGILSPWKGL LLYGPPGTGK
TLLAKAVATE CKTTFFNISA STIVSKWRGD SEKLVRVLFE LARYHAPSTI FLDELESVMS
QRGTASGGEH EGSLRMKTEL LVQMDGLARS EDLVFVLAAS NLPWELDCAM LRRLEKRILV
DLPSREARQA MIYHWLPPVS KSRALELHTE LEYSVLSQET EGYSGSDIKL VCREAAMRPV
RKIFDALENH QSESSDLPRI QLDIVTTADF LDVLTHTKPS AKNLAQRYSD WQREFESV
