ID   KAX27_CENLI             Reviewed;          36 AA.
AC   P45630;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Potassium channel toxin alpha-KTx 2.7;
DE   AltName: Full=C.1.limpidus toxin 2 {ECO:0000303|PubMed:7998956};
DE            Short=CllTx2 {ECO:0000303|PubMed:7998956};
DE   AltName: Full=Toxin II.10.9.2 {ECO:0000303|PubMed:7998956};
DE   Flags: Fragment;
OS   Centruroides limpidus (Mexican scorpion).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Centruroides.
OX   NCBI_TaxID=6876;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND FUNCTION.
RC   TISSUE=Venom;
RX   PubMed=7998956; DOI=10.1042/bj3040051;
RA   Martin B.M., Ramirez A.N., Gurrola G.B., Nobile M., Prestipino G.,
RA   Possani L.D.;
RT   "Novel K(+)-channel-blocking toxins from the venom of the scorpion
RT   Centruroides limpidus limpidus Karsch.";
RL   Biochem. J. 304:51-56(1994).
CC   -!- FUNCTION: Inhibitor of voltage-gated potassium channels (Kv). This
CC       protein is capable of displacing the binding of radio-labeled
CC       noxiustoxin (AC P08815) to rat brain synaptosomes with high affinity
CC       (about 100 pM). It is also capable of inhibiting transient potassium-
CC       currents (resembling I(A)-type currents), in cultured rat cerebellar
CC       granule cells. About 50% of the peak currents are reduced by
CC       application of a 1.5 uM solution of this toxin. Is lethal to mice (when
CC       less than 100 ug are injected). {ECO:0000269|PubMed:7998956}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:7998956}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:7998956}.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to a
CC       beta-sheet by disulfide bonds (CSalpha/beta).
CC       {ECO:0000250|UniProtKB:P59847}.
CC   -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC       channel inhibitor family. Alpha-KTx 02 subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P45630; -.
DR   SMR; P45630; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR001947; Scorpion_toxinS_K_inh.
DR   Pfam; PF00451; Toxin_2; 1.
DR   PRINTS; PR00286; CHARYBDTOXIN.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS01138; SCORP_SHORT_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Calcium-activated potassium channel impairing toxin;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Potassium channel impairing toxin; Secreted; Toxin.
FT   CHAIN           1..>36
FT                   /note="Potassium channel toxin alpha-KTx 2.7"
FT                   /id="PRO_0000066832"
FT   SITE            28
FT                   /note="Basic residue of the functional dyad"
FT                   /evidence="ECO:0000250"
FT   DISULFID        7..29
FT                   /evidence="ECO:0000250|UniProtKB:P59847"
FT   DISULFID        13..34
FT                   /evidence="ECO:0000250|UniProtKB:P59847"
FT   DISULFID        17..36
FT                   /evidence="ECO:0000250|UniProtKB:P59847"
FT   NON_TER         36
SQ   SEQUENCE   36 AA;  3912 MW;  C0350A262B6730C1 CRC64;
     TVIDVKCTSP KQCLPPCKEI YGRHAGAKCM NGKCKC