ID   KAX3C_ANDAM             Reviewed;          38 AA.
AC   P0C8R1;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Potassium channel toxin alpha-KTx 3.12;
DE   AltName: Full=Kaliotoxin analog;
DE            Short=Aam-KTX;
OS   Androctonus amoreuxi (African fattail scorpion) (Scorpio amoreuxi).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Androctonus.
OX   NCBI_TaxID=112024;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AND TOXIC DOSE.
RC   TISSUE=Venom;
RX   PubMed=18804453; DOI=10.1016/j.bbrc.2008.09.033;
RA   Abbas N., Belghazi M., Abdel-Mottaleb Y., Tytgat J., Bougis P.E.,
RA   Martin-Eauclaire M.-F.;
RT   "A new kaliotoxin selective towards Kv1.3 and Kv1.2 but not Kv1.1 channels
RT   expressed in oocytes.";
RL   Biochem. Biophys. Res. Commun. 376:525-530(2008).
CC   -!- FUNCTION: Potent inhibitor of voltage-dependent potassium channels,
CC       with a preference for Kv1.3/KCNA3 versus Kv1.2/KCNA2.
CC       {ECO:0000269|PubMed:18804453}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=4184.08; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:18804453};
CC   -!- TOXIC DOSE: LD(50) is 5 ug/kg by intracerebroventricular injection into
CC       mice (100 ng/mouse). {ECO:0000269|PubMed:18804453}.
CC   -!- MISCELLANEOUS: Has no effect on Kv1.1/KCNA1.
CC       {ECO:0000305|PubMed:18804453}.
CC   -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC       channel inhibitor family. Alpha-KTx 03 subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0C8R1; -.
DR   SMR; P0C8R1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR001947; Scorpion_toxinS_K_inh.
DR   Pfam; PF00451; Toxin_2; 1.
DR   PRINTS; PR00286; CHARYBDTOXIN.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS01138; SCORP_SHORT_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Potassium channel impairing toxin;
KW   Secreted; Toxin; Voltage-gated potassium channel impairing toxin.
FT   PEPTIDE         1..38
FT                   /note="Potassium channel toxin alpha-KTx 3.12"
FT                   /id="PRO_0000363663"
FT   MOD_RES         38
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000250"
FT   DISULFID        8..28
FT                   /evidence="ECO:0000250"
FT   DISULFID        14..33
FT                   /evidence="ECO:0000250"
FT   DISULFID        18..35
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   38 AA;  4192 MW;  11C0088FD6A021D4 CRC64;
     GVEINVKCTG SHQCIKPCKD AGMRFGKCIN RKCHCTPK