ID   KAX3G_MESGB             Reviewed;          60 AA.
AC   K7XFK5;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2013, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Potassium channel toxin alpha-KTx 3.16;
DE   AltName: Full=MegKTx4;
DE   AltName: Full=Tx4;
DE   Flags: Precursor;
OS   Mesobuthus gibbosus (Mediterranean checkered scorpion) (Buthus gibbosus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX   NCBI_TaxID=123226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=23142506; DOI=10.1016/j.toxicon.2012.10.010;
RA   Diego-Garcia E., Peigneur S., Debaveye S., Gheldof E., Tytgat J.,
RA   Caliskan F.;
RT   "Novel potassium channel blocker venom peptides from Mesobuthus gibbosus
RT   (Scorpiones: Buthidae).";
RL   Toxicon 61:72-82(2013).
CC   -!- FUNCTION: Potassium channel inhibitor. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC       channel inhibitor family. Alpha-KTx 03 subfamily. {ECO:0000305}.
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DR   EMBL; JX025015; AFX61611.1; -; mRNA.
DR   AlphaFoldDB; K7XFK5; -.
DR   SMR; K7XFK5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR001947; Scorpion_toxinS_K_inh.
DR   Pfam; PF00451; Toxin_2; 1.
DR   PRINTS; PR00286; CHARYBDTOXIN.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS01138; SCORP_SHORT_TOXIN; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Ion channel impairing toxin;
KW   Potassium channel impairing toxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..60
FT                   /note="Potassium channel toxin alpha-KTx 3.16"
FT                   /id="PRO_0000428965"
FT   DISULFID        30..50
FT                   /evidence="ECO:0000250"
FT   DISULFID        36..55
FT                   /evidence="ECO:0000250"
FT   DISULFID        40..57
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   60 AA;  6523 MW;  B95177FB963FC9F4 CRC64;
     MKVFSAVLII LFVCSMIIGI SEGKEIPVKC KHSGQCLQPC KDAGMRFGKC MNGKCNCTPK