ID   KAX3V_MESMA             Reviewed;          38 AA.
AC   P0DUK8;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=Toxin BmK NSPK {ECO:0000303|PubMed:33466524};
DE   AltName: Full=Buthus martensii Karsch neurite-stimulating peptide targeting Kv channels {ECO:0000303|PubMed:33466524};
OS   Mesobuthus martensii (Manchurian scorpion) (Buthus martensii).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX   NCBI_TaxID=34649;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, FUNCTION, MASS SPECTROMETRY, AND
RP   3D-STRUCTURE MODELING.
RC   TISSUE=Venom;
RX   PubMed=33466524; DOI=10.3390/toxins13010033;
RA   Zhao F., Zou X., Li S., He J., Xi C., Tang Q., Wang Y., Cao Z.;
RT   "BmK NSPK, a potent potassium channel inhibitor from scorpion Buthus
RT   martensii Karsch, promotes neurite outgrowth via NGF/TrkA signaling
RT   pathway.";
RL   Toxins 13:0-0(2021).
CC   -!- FUNCTION: Blocks voltage-gated potassium (Kv) channel and augments
CC       neurite extension via NGF/TrkA signaling pathway.
CC       {ECO:0000269|PubMed:33466524}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33466524}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:33466524}.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=3962.9; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:33466524};
CC   -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC       channel inhibitor family. Alpha-KTx 03 subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DUK8; -.
DR   SMR; P0DUK8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR001947; Scorpion_toxinS_K_inh.
DR   Pfam; PF00451; Toxin_2; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Potassium channel impairing toxin; Secreted; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   PEPTIDE         1..38
FT                   /note="Toxin BmK NSPK"
FT                   /evidence="ECO:0000269|PubMed:33466524"
FT                   /id="PRO_0000452716"
FT   DISULFID        7..27
FT                   /evidence="ECO:0000250|UniProtKB:P86396"
FT   DISULFID        13..32
FT                   /evidence="ECO:0000250|UniProtKB:P86396"
FT   DISULFID        17..34
FT                   /evidence="ECO:0000250|UniProtKB:P86396"
SQ   SEQUENCE   38 AA;  3966 MW;  4D3FA2EE2B72D7F8 CRC64;
     VGKNVICIHS GQCLIPCIDA GMRFGICKNG ICDCTPKG