ID   KAX55_HOTTA             Reviewed;          31 AA.
AC   P59870;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   26-SEP-2003, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Potassium channel toxin alpha-KTx 5.5;
DE   AltName: Full=Tamapin-2;
OS   Hottentotta tamulus (Eastern Indian scorpion) (Mesobuthus tamulus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX   NCBI_TaxID=34647;
RN   [1]
RP   PROTEIN SEQUENCE, AMIDATION AT HIS-31, MASS SPECTROMETRY, AND FUNCTION.
RC   TISSUE=Venom;
RX   PubMed=12239213; DOI=10.1074/jbc.m206465200;
RA   Pedarzani P., D'hoedt D., Doorty K.B., Wadsworth J.D.F., Joseph J.S.,
RA   Jeyaseelan K., Kini R.M., Gadre S.V., Sapatnekar S.M., Stocker M.,
RA   Strong P.N.;
RT   "Tamapin, a venom peptide from the Indian red scorpion (Mesobuthus tamulus)
RT   that targets small conductance Ca2+-activated K+ channels and
RT   afterhyperpolarization currents in central neurons.";
RL   J. Biol. Chem. 277:46101-46109(2002).
CC   -!- FUNCTION: Blocks small conductance calcium-activated potassium
CC       channels. {ECO:0000269|PubMed:12239213}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=3431.4; Mass_error=0.2; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:12239213};
CC   -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC       channel inhibitor family. Alpha-KTx 05 subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P59870; -.
DR   BMRB; P59870; -.
DR   SMR; P59870; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR001947; Scorpion_toxinS_K_inh.
DR   Pfam; PF00451; Toxin_2; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS01138; SCORP_SHORT_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Amidation; Calcium-activated potassium channel impairing toxin;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Potassium channel impairing toxin; Secreted; Toxin.
FT   PEPTIDE         1..31
FT                   /note="Potassium channel toxin alpha-KTx 5.5"
FT                   /id="PRO_0000044931"
FT   REGION          6..9
FT                   /note="[R/K]XCQ motif"
FT                   /evidence="ECO:0000305"
FT   MOD_RES         31
FT                   /note="Histidine amide"
FT                   /evidence="ECO:0000305|PubMed:12239213"
FT   DISULFID        3..21
FT                   /evidence="ECO:0000250|UniProtKB:P16341"
FT   DISULFID        8..26
FT                   /evidence="ECO:0000250|UniProtKB:P16341"
FT   DISULFID        12..28
FT                   /evidence="ECO:0000250|UniProtKB:P16341"
SQ   SEQUENCE   31 AA;  3439 MW;  E4D43CDC54CA415C CRC64;
     AFCNLRRCEL SCRSLGLLGK CIGEECKCVP H