KAX69_OPICA
ID KAX69_OPICA Reviewed; 61 AA.
AC Q6XLL6;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 52.
DE RecName: Full=Potassium channel toxin alpha-KTx 6.9 {ECO:0000303|PubMed:14696198};
DE AltName: Full=OcKTx4 {ECO:0000303|PubMed:14696198};
DE Flags: Precursor;
OS Opistophthalmus carinatus (African yellow leg scorpion).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Iurida; Scorpionoidea; Scorpionidae; Opistophthalminae;
OC Opistophthalmus.
OX NCBI_TaxID=190115;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=14696198; DOI=10.1002/prot.10588;
RA Zhu S.-Y., Huys I., Dyason K., Verdonck F., Tytgat J.;
RT "Evolutionary trace analysis of scorpion toxins specific for K-channels.";
RL Proteins 54:361-370(2004).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 24-61, SYNTHESIS OF 24-61, AND
RP DISULFIDE BONDS.
RX PubMed=29483648; DOI=10.1038/s41594-018-0033-9;
RA Correnti C.E., Gewe M.M., Mehlin C., Bandaranayake A.D., Johnsen W.A.,
RA Rupert P.B., Brusniak M.Y., Clarke M., Burke S.E., De Van Der Schueren W.,
RA Pilat K., Turnbaugh S.M., May D., Watson A., Chan M.K., Bahl C.D.,
RA Olson J.M., Strong R.K.;
RT "Screening, large-scale production and structure-based classification of
RT cystine-dense peptides.";
RL Nat. Struct. Mol. Biol. 25:270-278(2018).
CC -!- FUNCTION: Inhibits Kv1.2/KCNA2 and Kv1.3/KCNA3 voltage-gated potassium
CC channels (PubMed:29483648). {ECO:0000269|PubMed:29483648}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:14696198}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:14696198}.
CC -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC channel inhibitor family. Alpha-KTx 06 subfamily. {ECO:0000305}.
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DR EMBL; AY225782; AAP73820.1; -; mRNA.
DR PDB; 6AVD; X-ray; 1.80 A; A=24-61.
DR PDBsum; 6AVD; -.
DR AlphaFoldDB; Q6XLL6; -.
DR SMR; Q6XLL6; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR001947; Scorpion_toxinS_K_inh.
DR Pfam; PF00451; Toxin_2; 1.
DR PRINTS; PR00286; CHARYBDTOXIN.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS01138; SCORP_SHORT_TOXIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW Potassium channel impairing toxin; Secreted; Signal; Toxin;
KW Voltage-gated potassium channel impairing toxin.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..61
FT /note="Potassium channel toxin alpha-KTx 6.9"
FT /id="PRO_0000227034"
FT SITE 49
FT /note="Basic residue of the functional dyad"
FT /evidence="ECO:0000250|UniProtKB:Q10726"
FT SITE 58
FT /note="Aromatic residue of the functional dyad"
FT /evidence="ECO:0000250|UniProtKB:Q10726"
FT DISULFID 29..50
FT /evidence="ECO:0000269|PubMed:29483648,
FT ECO:0000312|PDB:6AVD"
FT DISULFID 35..55
FT /evidence="ECO:0000269|PubMed:29483648,
FT ECO:0000312|PDB:6AVD"
FT DISULFID 39..57
FT /evidence="ECO:0000269|PubMed:29483648,
FT ECO:0000312|PDB:6AVD"
FT DISULFID 45..60
FT /evidence="ECO:0000269|PubMed:29483648,
FT ECO:0000312|PDB:6AVD"
FT HELIX 32..34
FT /evidence="ECO:0007829|PDB:6AVD"
FT HELIX 36..43
FT /evidence="ECO:0007829|PDB:6AVD"
FT STRAND 48..51
FT /evidence="ECO:0007829|PDB:6AVD"
FT STRAND 54..57
FT /evidence="ECO:0007829|PDB:6AVD"
SQ SEQUENCE 61 AA; 6589 MW; E6F9A5179E74C640 CRC64;
MNAKFILLLL VVTTTTLLPD AKGAEIIRCS GTRECYAPCQ KLTGCLNAKC MNKACKCYGC
V