KAX6H_OPICY
ID KAX6H_OPICY Reviewed; 34 AA.
AC P86116;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2013, sequence version 2.
DT 25-MAY-2022, entry version 28.
DE RecName: Full=Potassium channel toxin alpha-KTx 6.17;
DE AltName: Full=Toxin OcyKTx2 {ECO:0000303|PubMed:23684923};
OS Opisthacanthus cayaporum (South American scorpion).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Iurida; Scorpionoidea; Hemiscorpiidae; Opisthacanthus.
OX NCBI_TaxID=573324;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=23684923; DOI=10.1016/j.peptides.2013.04.021;
RA Schwartz E.F., Bartok A., Schwartz C.A., Papp F., Gomez-Lagunas F.,
RA Panyi G., Possani L.D.;
RT "OcyKTx2, a new K-channel toxin characterized from the venom of the
RT scorpion Opisthacanthus cayaporum.";
RL Peptides 46:40-46(2013).
RN [2]
RP PROTEIN SEQUENCE OF 1-31, AND MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=18502464; DOI=10.1016/j.toxicon.2008.03.029;
RA Schwartz E.F., Camargos T.S., Zamudio F.Z., Silva L.P., Bloch C. Jr.,
RA Caixeta F., Schwartz C.A., Possani L.D.;
RT "Mass spectrometry analysis, amino acid sequence and biological activity of
RT venom components from the Brazilian scorpion Opisthacanthus cayaporum.";
RL Toxicon 51:1499-1508(2008).
CC -!- FUNCTION: This toxin reversibly blocks Shaker B potassium-channels
CC (expressed in insect Sf9 cells) with a Kd of 96.6 nM, and presents an
CC even better affinity toward hKv1.3 (KCNA3), blocking it with a Kd of
CC 17.7 nM. {ECO:0000269|PubMed:23684923}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18502464}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000269|PubMed:18502464}.
CC -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=3807; Method=Electrospray; Note=Monoisotopic
CC mass.; Evidence={ECO:0000269|PubMed:23684923};
CC -!- MASS SPECTROMETRY: Mass=3875; Method=Electrospray; Note=mass also found
CC by MALDI.; Evidence={ECO:0000269|PubMed:18502464};
CC -!- MISCELLANEOUS: Protein described in PubMed:18502464 elutes at 21.73
CC min., whereas protein described in PubMed:23684923 elutes at 21.22 min.
CC -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC channel inhibitor family. Alpha-KTx 06 subfamily. {ECO:0000255}.
CC -!- CAUTION: Peptides from PubMed:23684923 and PubMed:18502464 are
CC different since they are different in elution time and mass. They are
CC shown in a single entry until we know the reason of the differences
CC (eg. sequence or PTMs). {ECO:0000305}.
CC -!- CAUTION: Peptide described in PubMed:23684923 is identical to AC P86115
CC in mass and elution time, but not in sequence (2 amino acids are
CC different). {ECO:0000305}.
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DR AlphaFoldDB; P86116; -.
DR SMR; P86116; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR001947; Scorpion_toxinS_K_inh.
DR Pfam; PF00451; Toxin_2; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS01138; SCORP_SHORT_TOXIN; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Potassium channel impairing toxin; Secreted; Toxin.
FT PEPTIDE 1..34
FT /note="Potassium channel toxin alpha-KTx 6.17"
FT /evidence="ECO:0000269|PubMed:23684923"
FT /id="PRO_0000398131"
FT DISULFID 3..24
FT /evidence="ECO:0000250|UniProtKB:Q10726"
FT DISULFID 9..29
FT /evidence="ECO:0000250|UniProtKB:Q10726"
FT DISULFID 13..31
FT /evidence="ECO:0000250|UniProtKB:Q10726"
FT DISULFID 19..34
FT /evidence="ECO:0000250|UniProtKB:Q10726"
SQ SEQUENCE 34 AA; 3817 MW; DFA5467C9238613E CRC64;
IRCQGSNQCY GHCREKTGCM NGKCINRVCK CYGC