ID   KAX86_MESEU             Reviewed;          57 AA.
AC   P86400; E4VP12;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   31-JAN-2018, sequence version 2.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Potassium channel toxin alpha-KTx 8.6 {ECO:0000303|PubMed:25792741};
DE   AltName: Full=MeuTXKalpha1 {ECO:0000303|PubMed:20889474};
DE   AltName: Full=Toxin MeuKTx-1 {ECO:0000303|Ref.4};
DE   AltName: Full=meuK-toxin {ECO:0000312|EMBL:AMX81494.1};
DE   AltName: Full=pMeKTx1-1 {ECO:0000312|EMBL:AIL48757.1};
DE   Flags: Precursor;
OS   Mesobuthus eupeus (Lesser Asian scorpion) (Buthus eupeus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX   NCBI_TaxID=34648;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-33, FUNCTION,
RP   SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND 3D-STRUCTURE MODELING.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=20889474; DOI=10.1074/mcp.m110.002832;
RA   Zhu S., Peigneur S., Gao B., Luo L., Jin D., Zhao Y., Tytgat J.;
RT   "Molecular diversity and functional evolution of scorpion potassium channel
RT   toxins.";
RL   Mol. Cell. Proteomics 10:M110.002832-M110.002832(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Kuzmenkov A.I., Vassilevski A.A., Grishin E.V.;
RT   "Variability of potassium channel blockers in Mesobuthus eupeus.";
RL   Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Oliw E.H.;
RL   Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 29-57, FUNCTION, SUBCELLULAR LOCATION, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom;
RA   Zhu S.Y., Gao B.;
RT   "Characterization of a potassium channel toxin from the scorpion Mesobuthus
RT   eupeus venom.";
RL   Submitted (NOV-2009) to UniProtKB.
RN   [5]
RP   NOMENCLATURE.
RX   PubMed=25792741; DOI=10.1074/jbc.m115.637611;
RA   Kuzmenkov A.I., Vassilevski A.A., Kudryashova K.S., Nekrasova O.V.,
RA   Peigneur S., Tytgat J., Feofanov A.V., Kirpichnikov M.P., Grishin E.V.;
RT   "Variability of potassium channel blockers in Mesobuthus eupeus scorpion
RT   venom with focus on Kv1.1: an integrated transcriptomic and proteomic
RT   study.";
RL   J. Biol. Chem. 290:12195-12209(2015).
CC   -!- FUNCTION: Voltage-gated potassium channel antagonist. Has a high
CC       potency on Kv1.3/KCNA3, and is moderately active on Kv1.1/KCNA1 (35%
CC       peak current inhibition at 3 uM of toxin) and on Shaker IR potassium
CC       channels (70% peak current inhibition at 3 uM of toxin).
CC       {ECO:0000269|PubMed:20889474, ECO:0000269|Ref.4}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20889474}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:20889474}.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to a
CC       beta-sheet by disulfide bonds (CSalpha/beta).
CC       {ECO:0000250|UniProtKB:Q9U8D2}.
CC   -!- MASS SPECTROMETRY: Mass=3251; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:20889474, ECO:0000269|Ref.4};
CC   -!- MISCELLANEOUS: Does not inhibit rKv1.1/KCNA1, rKv1.2/KCNA2,
CC       rKv1.4/KCNA4, rKv1.5/KCNA5, rKv1.6/KCNA6, hKv11.1/KCNH2 (when tested at
CC       3 uM). {ECO:0000269|PubMed:20889474}.
CC   -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC       channel inhibitor family. Alpha-KTx 08 subfamily. {ECO:0000255}.
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DR   EMBL; EF442060; ABR20125.1; -; mRNA.
DR   EMBL; KF612499; AIL48757.1; -; mRNA.
DR   EMBL; KU569307; AMX81494.1; -; mRNA.
DR   AlphaFoldDB; P86400; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR008911; Toxin_alpha-KTx_8/9.
DR   Pfam; PF05453; Toxin_6; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Potassium channel impairing toxin; Secreted; Signal; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..28
FT                   /evidence="ECO:0000269|PubMed:20889474"
FT                   /id="PRO_0000442923"
FT   PEPTIDE         29..57
FT                   /note="Potassium channel toxin alpha-KTx 8.6"
FT                   /evidence="ECO:0000269|PubMed:20889474"
FT                   /id="PRO_0000401115"
FT   DISULFID        31..47
FT                   /evidence="ECO:0000250|UniProtKB:Q9U8D2"
FT   DISULFID        34..52
FT                   /evidence="ECO:0000250|UniProtKB:Q9U8D2"
FT   DISULFID        38..54
FT                   /evidence="ECO:0000250|UniProtKB:Q9U8D2"
SQ   SEQUENCE   57 AA;  6435 MW;  A6F7559B6214A252 CRC64;
     MSRLYAIILI ALVFNVIMTI MPDMKVEAVS CEDCPEHCAT KDQRAKCDND KCVCEPK