KAX92_MESMA
ID KAX92_MESMA Reviewed; 56 AA.
AC Q9U8D1;
DT 19-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Potassium channel toxin alpha-KTx 9.2 {ECO:0000305};
DE AltName: Full=Neurotoxin BmP03 {ECO:0000303|PubMed:10471839};
DE Flags: Precursor;
OS Mesobuthus martensii (Manchurian scorpion) (Buthus martensii).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX NCBI_TaxID=34649;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Venom gland;
RX PubMed=10386622; DOI=10.1016/s0014-5793(99)00651-1;
RA Wu J.-J., Dai L., Lan Z.-D., Chi C.-W.;
RT "Genomic organization of three neurotoxins active on small conductance
RT Ca2+-activated potassium channels from the scorpion Buthus martensi
RT Karsch.";
RL FEBS Lett. 452:360-364(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=10471839; DOI=10.1016/s0014-5793(99)01101-1;
RA Zhu S.-Y., Li W.-X., Zeng X.-C., Jiang D.-H., Mao X., Liu H.;
RT "Molecular cloning and sequencing of two 'short chain' and two 'long chain'
RT K(+) channel-blocking peptides from the Chinese scorpion Buthus martensii
RT Karsch.";
RL FEBS Lett. 457:509-514(1999).
RN [3]
RP PROTEIN SEQUENCE OF 29-56, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RX PubMed=9151979; DOI=10.1111/j.1432-1033.1997.00457.x;
RA Romi-Lebrun R., Martin-Eauclaire M.-F., Escoubas P., Wu F.Q., Lebrun B.,
RA Hisada M., Nakajima T.;
RT "Characterization of four toxins from Buthus martensi scorpion venom, which
RT act on apamin-sensitive Ca2+-activated K+ channels.";
RL Eur. J. Biochem. 245:457-464(1997).
RN [4]
RP STRUCTURE BY NMR OF 29-56, AND DISULFIDE BONDS.
RA He F., Li Y., Wu G., Cao C., Wu H.;
RT "Three-dimensional structure of BmP03 from venom of scorpion Buthus
RT martensii Karsch.";
RL Acta Chim. Sin. 58:850-855(2000).
CC -!- FUNCTION: Blocks small conductance calcium-activated potassium channels
CC (KCNN, SK). Low toxicity by intracerebroventricular injection into
CC mice.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9151979}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:9151979}.
CC -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to a
CC beta-sheet by disulfide bonds (CSalpha/beta). {ECO:0000269|Ref.4}.
CC -!- MASS SPECTROMETRY: Mass=2935.29; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:9151979};
CC -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC channel inhibitor family. Alpha-KTx 09 subfamily. {ECO:0000305}.
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DR EMBL; AF097408; AAF01253.1; -; Genomic_DNA.
DR EMBL; AF156170; AAF29463.1; -; mRNA.
DR PDB; 1WM8; NMR; -; A=29-56.
DR PDBsum; 1WM8; -.
DR AlphaFoldDB; Q9U8D1; -.
DR SMR; Q9U8D1; -.
DR EvolutionaryTrace; Q9U8D1; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR008911; Toxin_alpha-KTx_8/9.
DR Pfam; PF05453; Toxin_6; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium-activated potassium channel impairing toxin;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Neurotoxin; Potassium channel impairing toxin; Secreted; Signal; Toxin.
FT SIGNAL 1..28
FT /evidence="ECO:0000269|PubMed:9151979"
FT PEPTIDE 29..56
FT /note="Potassium channel toxin alpha-KTx 9.2"
FT /evidence="ECO:0000269|PubMed:9151979"
FT /id="PRO_0000035350"
FT DISULFID 31..47
FT /evidence="ECO:0000269|Ref.4, ECO:0000312|PDB:1WM8"
FT DISULFID 34..52
FT /evidence="ECO:0000269|Ref.4, ECO:0000312|PDB:1WM8"
FT DISULFID 38..54
FT /evidence="ECO:0000269|Ref.4, ECO:0000312|PDB:1WM8"
FT HELIX 36..38
FT /evidence="ECO:0007829|PDB:1WM8"
FT STRAND 40..42
FT /evidence="ECO:0007829|PDB:1WM8"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:1WM8"
SQ SEQUENCE 56 AA; 6001 MW; 70953032007E8672 CRC64;
MSRLFTLVLI VLAMNVMMAI ISDPVVEAVG CEECPMHCKG KNANPTCDDG VCNCNV