KAX95_BUTOC
ID KAX95_BUTOC Reviewed; 28 AA.
AC P84744;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 25-MAY-2022, entry version 46.
DE RecName: Full=Potassium channel toxin alpha-KTx 9.5 {ECO:0000305};
DE AltName: Full=Kbot1 {ECO:0000303|PubMed:15165720};
OS Buthus occitanus tunetanus (Common European scorpion) (Buthus tunetanus).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC Scorpiones; Buthida; Buthoidea; Buthidae; Buthus.
OX NCBI_TaxID=6871;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AMIDATION AT VAL-28, AND
RP MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=15165720; DOI=10.1016/j.peptides.2004.02.017;
RA Mahjoubi-Boubaker B., Crest M., Khalifa R.B., Ayeb M.E., Kharrat R.;
RT "Kbot1, a three disulfide bridges toxin from Buthus occitanus tunetanus
RT venom highly active on both SK and Kv channels.";
RL Peptides 25:637-645(2004).
CC -!- FUNCTION: Blocks voltage-gated potassium channels Kv1.1/KCNA1
CC (IC(50)=145 nM), Kv1.2/KCNA2 (IC(50)=2.5 nM), and Kv1.3/KCNA3
CC (IC(50)=15). Also inhibits calcium-activated potassium channels
CC (KCa/KCNN). {ECO:0000269|PubMed:15165720}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15165720}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:15165720}.
CC -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to a
CC beta-sheet by disulfide bonds (CSalpha/beta).
CC {ECO:0000250|UniProtKB:Q9NJP7}.
CC -!- MASS SPECTROMETRY: Mass=2942.58; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:15165720};
CC -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC channel inhibitor family. Alpha-KTx 09 subfamily. {ECO:0000305}.
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DR AlphaFoldDB; P84744; -.
DR SMR; P84744; -.
DR GO; GO:0005576; C:extracellular region; NAS:UniProtKB.
DR GO; GO:0019870; F:potassium channel inhibitor activity; IDA:UniProtKB.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0043267; P:negative regulation of potassium ion transport; IDA:UniProtKB.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR InterPro; IPR008911; Toxin_alpha-KTx_8/9.
DR Pfam; PF05453; Toxin_6; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
PE 1: Evidence at protein level;
KW Amidation; Calcium-activated potassium channel impairing toxin;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Neurotoxin; Potassium channel impairing toxin; Secreted; Toxin;
KW Voltage-gated potassium channel impairing toxin.
FT PEPTIDE 1..28
FT /note="Potassium channel toxin alpha-KTx 9.5"
FT /evidence="ECO:0000269|PubMed:15165720"
FT /id="PRO_0000045198"
FT MOD_RES 28
FT /note="Valine amide"
FT /evidence="ECO:0000269|PubMed:15165720"
FT DISULFID 3..19
FT /evidence="ECO:0000250|UniProtKB:Q9NJP7"
FT DISULFID 6..24
FT /evidence="ECO:0000250|UniProtKB:Q9NJP7"
FT DISULFID 10..26
FT /evidence="ECO:0000250|UniProtKB:Q9NJP7"
SQ SEQUENCE 28 AA; 2949 MW; DD72AD78A710B98B CRC64;
VGCEECPMHC KGKHAVPTCD DGVCNCNV
