ID   KAX_BUTOS               Reviewed;          60 AA.
AC   B8XH38;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Potassium channel toxin-like Tx677 {ECO:0000312|EMBL:ACJ23147.1};
DE   Flags: Precursor;
OS   Buthus occitanus israelis (Common yellow scorpion) (Buthus israelis).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Buthus.
OX   NCBI_TaxID=539894;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Zilberberg N., Kozminsky-Atias A.;
RT   "Buthus occitanus israelis scorpion toxin.";
RL   Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND SYNTHESIS OF 23-60.
RX   PubMed=29483648; DOI=10.1038/s41594-018-0033-9;
RA   Correnti C.E., Gewe M.M., Mehlin C., Bandaranayake A.D., Johnsen W.A.,
RA   Rupert P.B., Brusniak M.Y., Clarke M., Burke S.E., De Van Der Schueren W.,
RA   Pilat K., Turnbaugh S.M., May D., Watson A., Chan M.K., Bahl C.D.,
RA   Olson J.M., Strong R.K.;
RT   "Screening, large-scale production and structure-based classification of
RT   cystine-dense peptides.";
RL   Nat. Struct. Mol. Biol. 25:270-278(2018).
CC   -!- FUNCTION: Weakly inhibits Kv11.1/KCNH2/ERG1, Kv1.2/KCNA2 and
CC       Kv1.3/KCNA3 voltage-gated potassium channels.
CC       {ECO:0000269|PubMed:29483648}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|Ref.1}.
CC   -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium
CC       channel inhibitor family. {ECO:0000305}.
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DR   EMBL; FJ360827; ACJ23147.1; -; mRNA.
DR   AlphaFoldDB; B8XH38; -.
DR   SMR; B8XH38; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR001947; Scorpion_toxinS_K_inh.
DR   Pfam; PF00451; Toxin_2; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS01138; SCORP_SHORT_TOXIN; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Ion channel impairing toxin;
KW   Potassium channel impairing toxin; Secreted; Signal; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..60
FT                   /note="Potassium channel toxin-like Tx677"
FT                   /id="PRO_0000405977"
FT   SITE            50
FT                   /note="Basic residue of the functional dyad"
FT                   /evidence="ECO:0000250"
FT   SITE            59
FT                   /note="Aromatic residue of the functional dyad"
FT                   /evidence="ECO:0000250"
FT   DISULFID        30..51
FT                   /evidence="ECO:0000250"
FT   DISULFID        36..56
FT                   /evidence="ECO:0000250"
FT   DISULFID        40..58
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   60 AA;  6800 MW;  00F19F0D2BBFF96E CRC64;
     MKISALVMIT LLICSMMILC QGQKILSNRC NNSSECIPHC IRIFGTRAAK CINRKCYCYP