ID   KBRS2_XENLA             Reviewed;         163 AA.
AC   Q32NS2;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=NF-kappa-B inhibitor-interacting Ras-like protein 2;
DE   AltName: Full=I-kappa-B-interacting Ras-like protein 2;
DE            Short=Kappa B-Ras protein 2;
DE            Short=KappaB-Ras2;
GN   Name=nkiras2;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Atypical Ras-like protein that acts as a potent regulator of
CC       NF-kappa-B activity by preventing the degradation of NF-kappa-B
CC       inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more
CC       resistant to degradation. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DOMAIN: In contrast to other members of the Ras family, the members of
CC       the KappaB-Ras subfamily do not contain the conserved Gly and Gln
CC       residues in positions 13 and 65, which are replaced by His and Leu
CC       residues, respectively, and are therefore similar to the constitutively
CC       active forms of oncogenic forms of Ras. This suggests that members of
CC       this family are clearly different from other small GTPases proteins.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Ras family.
CC       KappaB-Ras subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC108504; AAI08505.1; -; mRNA.
DR   RefSeq; NP_001089825.1; NM_001096356.1.
DR   AlphaFoldDB; Q32NS2; -.
DR   SMR; Q32NS2; -.
DR   DNASU; 734891; -.
DR   GeneID; 734891; -.
DR   KEGG; xla:734891; -.
DR   CTD; 734891; -.
DR   Xenbase; XB-GENE-6254992; nkiras2.L.
DR   OrthoDB; 1382000at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10L.
DR   Bgee; 734891; Expressed in internal ear and 19 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0007249; P:I-kappaB kinase/NF-kappaB signaling; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR042227; KBRS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR001806; Small_GTPase.
DR   PANTHER; PTHR46152; PTHR46152; 1.
DR   Pfam; PF00071; Ras; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51419; RAB; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; GTP-binding; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..163
FT                   /note="NF-kappa-B inhibitor-interacting Ras-like protein 2"
FT                   /id="PRO_0000225683"
FT   REGION          1..163
FT                   /note="Small GTPase-like"
FT   MOTIF           35..43
FT                   /note="Effector region"
FT   BINDING         11..18
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         61..65
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         120..123
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   163 AA;  18398 MW;  2DA79860FD32EB03 CRC64;
     MGKSCKVVIC GQHGVGKTSI LEQLLYGNHV VGSDMIETQE DIYIGSVETD RGVREQVRFY
     DTRGLKDGLE LPKHCFCGTD GYVLVYSVDN KDSFKRVEAL KKEIDRSKDK KEVTIVVLGN
     KSDMKDQRRV DHEAAQQWAK AEKILLRNGP THGSANWNLH PDH